Title: Mechanism for the formation of 2,3-butanediol stereoisomers in Bacillus polymyxa
Abstract: The mechanism of the formation of 2,3-butanediol isomers in Bacillus polymyxa was studied. We proposed a new model with NADPH-linked diacetyl reductase (S-acetoin forming) and R(−)-2,3-butanediol dehydrogenase. The two enzymes were separated by Blue Sepharose CL-6B and their stereospecificities were identified using all of the pure isomers of 2,3-butanediol (R(−), S(+)m, and meso), acetoin (R(−) and S(+)) and the separation and measurement of these isomers. The presence of acetoin or butanediol racemase was not confirmed in our experiments.
Publication Year: 1986
Publication Date: 1986-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 55
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