Publication Information

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2128, 2135, 2162, 2192, 2233, 2273, 2282, 2294, 2307, 2314, 2322, 2326, 2351, 2355, 2361, 2368, 2387, 2391, 2405, 2441, 2450, 2454, 2487, 2520, 2532, 2554, 2572, 2608, 2634, 2656, 2747, 2796, 2805, 2822, 2827, 2842, 2891, 2930, 2940, 2957, 2967, 2971, 3046, 3067, 3125, 3161, 3212, 3220, 3258, 3265, 3282, 3328], 'contractile': [25, 59, 113, 222, 256, 310, 496, 692, 941, 960, 978, 1660, 1787, 2931, 3127], 'force': [26, 131, 223, 328, 497, 523, 693, 824, 945, 979, 1085, 1110, 1138, 1733, 1788, 2129, 2196, 2932, 2958, 3128, 3259], 'by': [27, 224, 440, 447, 529, 564, 634, 801, 991, 1022, 1111, 1139, 1185, 1198, 1226, 1272, 1363, 1391, 1403, 1473, 1703, 1793, 1862, 1921, 1997, 2160, 2182, 2187, 2204, 2228, 2280, 2297, 2371, 2668, 2702, 2875, 2882, 2964, 2999, 3069, 3236], 'cGMP.': [28, 225, 1267, 1392], 'The': [29, 139, 173, 226, 336, 370, 614, 1485, 1505, 2152, 2341, 2365, 2641, 3049], 'nonhydrolyzable': [30, 227, 870, 3305], 'analog': [31, 228], '8-bromo-cGMP,': [32, 229], 'when': [33, 230], 'applied': [34, 231], 'to': [35, 66, 232, 263, 487, 524, 628, 642, 694, 819, 875, 935, 943, 955, 968, 980, 1001, 1076, 1091, 1156, 1355, 1384, 1460, 1545, 1553, 1563, 1577, 1583, 1762, 2085, 2133, 2304, 2348, 2425, 2529, 2569, 2584, 2808, 2922, 2959, 3063, 3077, 3129, 3254, 3310], 'these': [36, 233], 'with': [38, 61, 235, 258, 1454, 1592, 1668, 2002, 2212, 2511], 'submaximal': [39, 236], 'levels': [41, 50, 238, 247, 483, 2197, 2671, 2705, 2918], 'clamped,': [42, 239], 'dramatically': [43, 240], 'and': [44, 58, 86, 112, 130, 241, 255, 283, 309, 327, 402, 444, 491, 609, 686, 763, 771, 781, 849, 928, 1028, 1084, 1148, 1192, 1259, 1379, 1410, 1449, 1503, 1518, 1540, 1552, 1710, 1725, 1760, 1826, 1900, 1918, 1988, 2083, 2154, 2164, 2186, 2224, 2311, 2373, 2480, 2613, 2636, 2651, 2661, 2672, 2706, 2800, 2837, 2879, 2926, 3044, 3121, 3198, 3206, 3216, 3284], 'reversibly': [45, 242, 929], 'reduced': [46, 243, 2202, 2384], 'steady': [48, 81, 137, 245, 278, 334, 1079], 'state': [49, 82, 246, 279, 1080, 1144], 'phosphorylation': [52, 85, 111, 142, 249, 282, 308, 339, 406, 538, 784, 990, 1083, 1120, 1178, 1222, 1240, 1269, 1286, 1408, 1754, 1767, 1880, 1885, 1905, 1959, 2021, 2077, 2092, 2137, 2168, 2633, 2841, 2973, 3219], 'at': [53, 95, 250, 292, 438, 993, 1121, 1181, 1223, 1241, 1281, 1906, 1914, 1924, 2079, 2169, 2873], '20-kDa': [54, 251, 410, 2845], 'myosin': [55, 252, 411, 420, 2025, 2846, 2855], 'light': [56, 89, 109, 116, 127, 140, 167, 184, 253, 286, 306, 313, 324, 337, 364, 381, 412, 421, 426, 429, 2847, 2856, 2861, 2864], 'chain': [57, 90, 110, 117, 128, 141, 168, 185, 254, 287, 307, 314, 325, 338, 365, 382, 413, 427, 430, 2848, 2862, 2865], 'force,': [60, 257, 1661], 'a': [62, 146, 161, 259, 343, 358, 599, 864, 984, 994, 999, 1014, 1093, 1235, 1242, 1494, 1598, 1699, 1735, 1756, 1866, 1883, 1943, 2229, 2283, 2818, 3034, 3299], 'nanomolar': [63, 260, 920, 2552], 'concentration': [64, 261], 'required': [65, 262], 'obtain': [67, 264], '50%': [68, 265], 'reduction.': [69, 266], 'Supramaximal': [70, 267], 'concentrations': [71, 268, 921, 2607], '8-bromo-cGMP': [73, 100, 124, 154, 270, 297, 321, 351, 873, 3308], '(10': [74, 271], 'μM),': [75, 272], 'however,': [76, 273, 519, 1368, 1530, 2954], 'did': [77, 101, 274, 298, 1394, 2394], 'not': [78, 102, 275, 299, 861, 925, 1074, 1280, 1288, 1331, 1350, 1395, 1685, 1811, 2042, 2395, 2446, 3296], 'change': [79, 276, 1000, 1077, 1396], 'relationship': [83, 280, 1081], 'between': [84, 281, 1082], 'force.': [87, 114, 284, 311, 1415], 'When': [88, 115, 285, 312], 'phosphatase': [91, 169, 288, 366, 449, 1446, 1837, 1861, 2884], 'activity': [92, 119, 170, 289, 316, 367, 489, 500, 762, 1291, 1336, 1375, 1398, 1557, 1745, 1803, 2924, 2935, 3197], 'was': [93, 120, 290, 317, 1418, 1477, 1509, 1696, 2131, 2180, 2346, 2378], 'blocked': [94, 291], 'pCa': [96, 147, 293, 344, 1752, 1771], '6.7,': [97, 294], '10': [98, 122, 295, 319, 2176], 'μM': [99, 123, 296, 320], 'affect': [103, 300, 2397, 2447], 'rates': [105, 302, 1500, 2156], 'rise': [107, 304], 'kinase': [118, 315, 432, 442, 1187, 1190, 1194, 1255, 1340, 2588, 2615, 2867, 2877], 'blocked,': [121, 318], 'significantly': [125, 322, 1497, 2396], 'accelerated': [126, 323], 'dephosphorylation': [129, 326, 446, 1502, 1508, 2149, 2155, 2339, 2363, 2443, 2881], 'relaxation': [132, 329, 403, 1522, 2142, 2153, 2274, 2295, 2327, 2369, 2392, 2525, 2701, 2838], 'from': [133, 160, 330, 357, 1322, 1597, 1777, 2549], 'maximal': [135, 332], 'contraction': [136, 149, 333, 346, 401, 1147, 2658, 2667, 2836], 'state.': [138, 335], 'time': [143, 340, 1590, 1712, 2088, 2126, 2271, 2366], 'course': [144, 341, 1713, 2089, 2127, 2272, 2367], '6.0-induced': [148, 345, 1753, 1772], 'in': [150, 164, 195, 347, 361, 392, 460, 605, 638, 661, 687, 826, 899, 946, 987, 1017, 1119, 1151, 1176, 1277, 1420, 1488, 1513, 1715, 1721, 1743, 1755, 1789, 1804, 1820, 1869, 1890, 1965, 1991, 2051, 2053, 2093, 2172, 2184, 2189, 2198, 2275, 2328, 2375, 2430, 2458, 2478, 2509, 2522, 2557, 2566, 2624, 2631, 2638, 2644, 2786, 2812, 2895, 3040, 3073, 3096, 3122, 3261, 3334], 'presence': [152, 349, 2191], 'exhibited': [155, 352], 'kinetics': [156, 353, 1586, 1714, 1748, 1768], 'that': [157, 180, 354, 377, 397, 886, 1105, 1150, 1175, 1252, 1328, 1387, 1436, 1442, 1475, 1631, 1650, 1665, 1691, 1727, 1780, 1801, 1818, 1857, 1941, 2134, 2289, 2332, 2436, 2464, 2476, 2560, 2604, 2789, 2832, 3321], 'predictable': [159, 356], 'mathematical': [162, 359], 'model': [163, 360, 1600], 'which': [165, 362, 509, 957, 2358, 2944], 'only': [166, 363, 862, 1942, 2075, 2203, 3297], 'is': [171, 368, 1089, 1113, 1172, 1330, 1389, 1438, 1531, 1582, 1632, 1810, 1910, 1949, 1960, 2081, 2318, 2359, 2508, 2574, 2627], 'increased.': [172, 369], 'results': [174, 371, 1646, 1779, 2214, 2428], 'this': [176, 373, 811, 858, 951, 1101, 1285, 1468, 2376, 2558, 2645, 2787, 3246, 3293], 'study': [177, 374, 917, 2377, 2559, 2788], 'strongly': [178, 375, 927], 'suggest': [179, 376, 1856], 'cGMP': [181, 378, 644, 657, 802, 831, 871, 887, 992, 1140, 1159, 1329, 1364, 1476, 1580, 1728, 1781, 1863, 2515, 2521, 2568, 2579, 2790, 3079, 3092, 3237, 3266, 3306, 3322], 'indirectly': [182, 379], 'activates': [183, 380, 888, 3323], 'phosphatase,': [186, 383], 'first': [188, 385], 'proposed': [189, 386, 795, 1251, 3230], 'mechanism': [190, 387], 'cGMP-induced': [192, 389, 975, 1015], 'vasodilatation.': [196, 393], 'INTRODUCTIONThe': [394, 2829], 'primary': [395, 2629, 2830], 'mechanisms': [396, 566, 729, 796, 1133, 2643, 2831, 3001, 3164, 3231], 'regulate': [398, 2833], 'are,': [404, 2839], 'respectively,': [405, 2840], 'regulatory': [409, 962, 1005, 2844], '(MLC20)': [414, 2849], '1The': [415, 2850], 'abbreviations': [416, 2851], 'used': [417, 2852], 'are:': [418, 2853], 'MLC2020-kDa': [419, 2854], 'chain8Br-cGMP8-bromo': [422, 2857], 'guanosine': [423, 2858], '3′,5′-cyclic': [424, 2859], 'monophosphateMLCKmyosin': [425, 2860], 'kinaseMLCPmyosin': [428, 2863], 'phosphataseMC-LRmicrocystin-LRML-91-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepinePKGprotein': [431, 2866], 'G': [433, 2868], 'or': [434, 563, 961, 1167, 1963, 2001, 2869, 2998], 'cGMP-activated': [435, 1254, 2870], 'kinaseGTPγSguanosine': [436, 2871], '5′-3-O-(thio)triphosphate.': [437, 2872], 'Ser-19': [439, 2874], 'MLC20': [441, 448, 537, 989, 1003, 1239, 1879, 1898, 1948, 2080, 2091, 2635, 2876, 2883, 2972], '(MLCK)': [443, 2878], 'its': [445, 786, 1182, 1338, 1404, 2880, 3221], '(MLCP)': [450, 2885], '(1Hartshorne': [451, 2886], 'D.J.': [452, 1930, 2030, 2887], 'Johnson': [453, 2888], 'L.R.': [454, 2889], 'Biochemistry': [455, 1975, 2061, 2890], 'Contractile': [458, 2893], 'Process': [459, 2894], 'Smooth': [461, 843, 1977, 2063, 2896, 3278], 'Muscle.': [462, 2897], 'Raven': [463, 2898], 'Press,': [464, 2899], 'New': [465, 2900], 'York1987:': [466, 2901], '423-482Google': [467, 2902], 'Scholar,': [468, 553, 582, 675, 710, 1040, 1050, 1306, 1617, 2112, 2686, 2724, 2765, 2903, 2988, 3017, 3110, 3145], '2Kamm': [469, 2904], 'K.E.': [470, 1213, 2771, 2905], 'Stull': [471, 2774, 2906], 'J.T.': [472, 1207, 2775, 2907], 'Annu.': [473, 2240, 2908], 'Rev.': [474, 2241, 2909], 'Physiol.': [475, 670, 2014, 2242, 2681, 2778, 2910, 3105], '1989;': [476, 651, 720, 2243, 2911, 3086, 3155], '51:': [477, 2244, 2912], '299-313Google': [478, 2913], 'Scholar).': [479, 654, 723, 856, 1069, 1220, 1320, 1854, 2018, 2073, 2246, 2505, 2543, 2737, 2914, 3089, 3158, 3291, 3351], 'Typically,': [480, 2915], 'modulate': [484, 2919], 'MLCK': [486, 499, 1180, 1197, 1203, 1258, 1271, 1293, 1335, 1348, 1362, 1388, 1542, 1556, 1701, 1892, 2921, 2934], 'MLCP': [488, 1374, 1432, 1651, 1680, 1744, 1802, 1922, 2573, 2748, 2823, 2923], 'ratio': [490, 2925], 'ultimately': [492, 2927], 'degree': [494, 2929], 'because': [498, 803, 1071, 2516, 2578, 2933, 3238], 'depends': [501, 2936], 'on': [502, 512, 810, 1346, 1406, 1634, 1882, 2937, 2947, 3245], 'amount': [504, 2939], 'Ca2+-calmodulin': [507, 1205, 2942], 'complex,': [508, 2943], 'itself': [510, 2945], 'hinges': [511, 2946], 'cytosolic': [513, 2467, 2948], 'levels.': [515, 2950], 'In': [516, 788, 857, 1100, 1954, 2951, 3223, 3292], 'many': [517, 2952], 'cases,': [518, 2953], 'sensitivity': [521, 689, 2655, 2675, 2709, 2956, 3124], 'can': [526, 1594, 1893, 2225, 2545, 2961], 'be': [527, 965, 1013, 1568, 1595, 1865, 2226, 2302, 2349, 2620, 2962], 'changed': [528, 1746, 1765, 2963], 'physiological': [530, 601, 1382, 1808, 1952, 2965, 3036], 'modulation': [531, 2966], 'dependence': [535, 2970], '(3Kitazawa': [539, 2974], 'T.': [540, 903, 1052, 1058, 1062, 1602, 1619, 1621, 2011, 2097, 2114, 2116, 2726, 2750, 2975, 3338], 'Gaylinn': [541, 2976], 'B.D.': [542, 2977], 'Denney': [543, 2978], 'G.H.': [544, 2979], 'Somlyo': [545, 556, 1605, 2100, 2253, 2259, 2411, 2417, 2753, 2980, 2991], 'A.P.': [546, 555, 1606, 2101, 2260, 2418, 2754, 2981, 2990], 'J.': [547, 576, 650, 669, 681, 700, 712, 742, 913, 1034, 1214, 1300, 1313, 1624, 1933, 2012, 2033, 2119, 2262, 2420, 2495, 2539, 2595, 2680, 2719, 2732, 2777, 2982, 3011, 3085, 3104, 3116, 3135, 3147, 3177, 3348], 'Biol.': [548, 577, 1035, 1064, 1215, 1301, 1625, 1934, 2034, 2120, 2596, 2983, 3012], 'Chem.': [549, 578, 1036, 1065, 1216, 1302, 1626, 1935, 2035, 2121, 2597, 2984, 3013], '1991;': [550, 579, 1037, 1614, 2109, 2762, 2985, 3014], '266:': [551, 580, 1038, 2986, 3015], '1708-1715Google': [552, 2987], '4Somlyo': [554, 2989], 'A.V.': [557, 2254, 2412, 2992], 'Nature.': [558, 1850, 2993], '1994;': [559, 1627, 2015, 2122, 2994], '372:': [560, 2995], '231-236Google': [561, 2996], 'Scholar)': [562, 685, 1630, 1987, 2039, 2223, 2266, 2424, 2997, 3120], 'other': [565, 2512, 3000], 'such': [567, 1097, 1465, 1493, 1679, 2469, 3002], 'as': [568, 598, 620, 622, 1401, 1562, 2470, 3003, 3033, 3055, 3057], 'thin': [569, 1024, 3004], 'filament': [570, 3005], 'disinhibition': [571, 3006], '(5Sobue': [572, 1030, 3007], 'K.': [573, 1031, 1970, 2713, 2717, 3008], 'Sellers': [574, 1032, 3009], 'J.R.': [575, 1033, 2058, 3010], '12115-12118Google': [581, 1039, 3016], '6Winder': [583, 1041, 3018], 'S.J.': [584, 1042, 3019], 'Walsh': [585, 1043, 3020], 'M.P.': [586, 1044, 3021], 'Cell': [587, 1045, 3022], 'Signalling.': [588, 1046, 3023], '1993;': [589, 682, 743, 1047, 1851, 2540, 3024, 3117, 3178], '5:': [590, 1048, 3025], '667-686Google': [591, 1049, 3026], 'Scholar).The': [592, 2125, 3027], 'nucleotide': [593, 3028], 'cyclic': [594, 3029], 'GMP': [595, 3030], 'has': [596, 658, 806, 816, 896, 1814, 2526, 2580, 3031, 3093, 3241, 3251, 3331], 'emerged': [597, 3032], 'potent,': [600, 3035], 'second': [602, 778, 2342, 3037, 3213], 'messenger': [603, 779, 3038, 3214], 'involved': [604, 2565, 3039], 'both': [606, 2199, 2611, 3041], 'vasodilator': [607, 3042], 'action': [608, 2519, 3043], 'failure': [610, 3045], 'vasoconstrictor': [612, 3047], 'activity.': [613, 3048], 'neighboring': [615, 3050], 'endothelium,': [616, 3051], 'endogenous': [617, 3052], 'circulating': [618, 3053], 'hormones,': [619, 3054], 'well': [621, 1173, 3056], 'clinically': [623, 3058], 'administered': [624, 3059], 'nitrovasodilators': [625, 2799, 3060], 'all': [626, 3061], 'function': [627, 1868, 2530, 3062], 'widen': [629, 3064], 'lumen': [631, 3066], 'vessels': [633, 3068], 'stimulating': [635, 3070], 'guanylyl': [636, 3071], 'cyclase': [637, 3072], 'vascular': [639, 938, 1790, 3074], 'produce': [643, 2666, 3078], '(7Furchgott': [645, 3080], 'R.F.': [646, 3081], 'Vanhoutte': [647, 3082], 'P.M.': [648, 3083], 'FASEB': [649, 680, 741, 2538, 3084, 3115, 3176], '3:': [652, 3087], '2007-2018Google': [653, 3088], 'To': [655, 3090], 'date,': [656, 3091], 'been': [659, 732, 807, 817, 1250, 1674, 1815, 2527, 2582, 3094, 3167, 3242, 3252], 'implicated': [660, 3095], 'lowering': [662, 728, 3097, 3163], '(8Morgan': [665, 3100], 'J.P.': [666, 3101], 'Morgan': [667, 2678, 3102], 'K.G.': [668, 2679, 3103], '(Lond.).': [671, 2682, 3106], '1984;': [672, 3107], '357:': [673, 3108], '539-551Google': [674, 3109], '9Lincoln': [676, 737, 3111, 3172], 'T.M.': [677, 738, 1845, 2535, 3112, 3173], 'Cornwell': [678, 739, 2536, 3113, 3174], 'T.L.': [679, 740, 2537, 3114, 3175], '7:': [683, 744, 2541, 3118, 3179], '328-338Google': [684, 745, 2542, 3119, 3180], 'decreasing': [688, 1199, 1675, 2707, 3123], '(10Pfitzer': [696, 2491, 3131], 'G.': [697, 2492, 3132], 'Merkel': [698, 2493, 3133], 'L.': [699, 907, 911, 2494, 2592, 3134, 3342, 3346], 'Rüegg': [701, 2496, 3136], 'J.C.': [702, 2497, 3137], 'Hoffman': [703, 2498, 3138], 'F.': [704, 833, 2499, 3139, 3268], 'Pflüger.': [705, 2500, 3140], 'Arch.': [706, 2501, 3141], '1986;': [707, 1936, 2036, 2502, 3142], '407:': [708, 2503, 3143], '87-91Google': [709, 2504, 3144], '11Nishimura': [711, 3146], 'van': [713, 3148], 'Breemen': [714, 3149], 'C.': [715, 3150], 'Biochem.': [716, 3151], 'Biophys.': [717, 912, 2261, 2419, 3152, 3347], 'Res.': [718, 2692, 3153], 'Commun.': [719, 3154], '163:': [721, 3156], '929-935Google': [722, 3157], 'A': [724, 890, 1184, 1225, 1378, 1451, 1705, 1740, 2616, 3159, 3325], 'myriad': [725, 3160], 'have': [730, 2784, 3165], 'since': [731, 3166], 'reportedly': [733, 3168], 'shown': [734, 2429, 2528, 2556, 2583, 2785, 3169], '(see': [735, 3170], 'Ref.': [736, 3171], 'Scholar': [746, 3181], 'references):': [748, 3183], 'increased': [749, 752, 767, 1498, 3184, 3187, 3202], 'sequestration,': [751, 3186], 'efflux,': [754, 3189], 'decreased': [755, 759, 772, 2181, 3190, 3194, 3207], 'influx': [757, 3192], 'through': [758, 764, 775, 783, 1116, 1337, 1481, 1734, 1830, 2745, 2794, 3193, 3199, 3210, 3218], 'channel': [761, 769, 3196, 3204], 'hyperpolarization': [765, 3200], 'via': [766, 2803, 3201], 'K+': [768, 3203], 'activity,': [770, 3205], 'release': [774, 3209], 'antagonism': [776, 3211], 'formation': [780, 3215], 'possibly': [782, 3217], 'receptor.': [787, 3222], 'stark': [789, 3224], 'contrast,': [790, 3225], 'there': [791, 3226], 'currently': [793, 3228], 'no': [794, 814, 2517, 3229, 3249], 'little': [804, 3239], 'research': [805, 1345, 3240], 'carried': [808, 3243], 'out': [809, 1127, 1155, 1687, 3244], 'effect.': [812, 3247], 'Still,': [813, 1342, 3248], 'one': [815, 2076, 2433, 3250], 'able': [818, 3253], 'belittle': [820, 3255], 'significance': [822, 3257], 'vasodilating': [828, 2698, 3263], 'effect': [829, 952, 1265, 1566, 1581, 2457, 2477, 3264], '(12Hofmann': [832, 3267], 'Ludwig': [834, 3269], 'A.': [835, 837, 1054, 1613, 1847, 2108, 2252, 2410, 2761, 3270, 3272], 'Pfeifer': [836, 3271], 'Raeburn': [838, 3273], 'D.': [839, 3274], 'Giembycz': [840, 3275], 'M.A.': [841, 3276], 'Airways': [842, 3277], 'Muscle:': [844, 3279], 'Biochemical': [845, 3280], 'Control': [846, 3281], 'Contraction': [848, 3283], 'Relaxation.': [850, 3285], 'Birkhauser': [851, 3286], 'Verlag,': [852, 3287], 'Basel,': [853, 3288], 'Switzerland1994:': [854, 3289], '253-269Google': [855, 3290], 'study,': [859, 3294], 'demonstrate': [863, 3298], 'very': [865, 1758, 1944, 3300], 'high': [866, 2605, 3301], 'affinity': [867, 1201, 3302], 'analog,': [872, 3307], '(8Br-cGMP)': [874, 3309], 'bring': [876, 3311], 'about': [877, 3312], 'desensitization,': [879, 3314], 'also': [882, 1642, 1799, 2379, 2581, 3317], 'provide': [883, 1351, 3318], 'convincing': [884, 3319], 'evidence': [885, 3320], 'MLCP.': [889, 1484, 1739, 1795, 2797, 2828, 3324], 'short': [891, 3326], 'report': [892, 3327], 'our': [894, 1443, 1588, 1669, 1692, 1872, 2094, 2427, 3329], 'work': [895, 2404, 2486, 3330], 'appeared': [897, 3332], 'previously': [898, 3333], 'abstract': [900, 3335], 'form': [901, 3336], '(13Kitazawa': [902, 3337], 'Lee': [904, 1840, 3339], 'M.': [905, 909, 1060, 1295, 1604, 1623, 1928, 1932, 1972, 1974, 2005, 2009, 2028, 2032, 2060, 2099, 2118, 2216, 2728, 2752, 2769, 3340, 3344], 'Zhang': [906, 3341], 'Masuo': [908, 1603, 2098, 2751, 3343], 'Li': [910, 3345], '1996;': [914, 3349], '70Google': [915, 3350], 'Scholar).DISCUSSIONThis': [916], 'illustrates': [918], 'how': [919, 1763], '8Br-cGMP': [923, 954, 1072, 1112, 1393, 1496, 1517, 1764, 2383, 2437, 2555, 2609], '8Br-5′GMP': [926], 'reduces': [930], 'ability': [932, 1457], 'cause': [936, 1234, 2700, 2742, 2791], 'machinery': [942], 'generate': [944], 'situ.': [947], 'Moreover,': [948], 'it': [949, 1088, 1103, 1248, 1326, 1437, 1470], 'analyzes': [950], 'identify': [956], 'apparatuses': [963], 'might': [964, 1567], 'altered': [966], 'sufficiently': [967], 'account': [969], 'desensitizing': [973, 1264, 2456], 'effect.The': [974], 'must': [982], 'involve': [983], 'Ca2+-independent': [985, 1117], 'reduction': [986], 'given': [995, 1243], 'level': [997, 2623], 'and/or': [998, 2472], 'some': [1002, 1168, 1560, 2465], 'phosphorylation-independent': [1004, 1098], 'mechanism(s).': [1006], 'An': [1007], 'example': [1008], 'latter': [1011, 1908], 'could': [1012, 1349, 1684], 'increase': [1016, 1742, 2043], 'inhibition': [1018, 1360, 1702, 2746, 2826], 'actomyosin': [1020], 'ATPase': [1021, 2026], 'filament-associated': [1025], 'proteins': [1026], 'caldesmon': [1027], 'calponin': [1029], '19Itoh': [1051], 'Suzuki': [1053], 'Watanabe': [1055], 'Y.': [1056, 2767], 'Mino': [1057], 'Naka': [1059], 'Tanaka': [1061], 'J': [1063], '1995;': [1066, 2263, 2421], '270:': [1067], '20400-20403Google': [1068], 'However,': [1070, 1123, 2577], 'does': [1073, 1274, 1287, 2041, 2445], 'appear': [1075, 2807], '(Fig.': [1086, 1376, 1640, 2138, 2150, 2206, 2381], '3C),': [1087], 'reasonable': [1090], 'exclude': [1092], 'significant': [1094], 'involvement': [1095], 'mechanisms.': [1099], 'tissue,': [1102], 'appears': [1104], 'mainly': [1114], 'mediated': [1115], 'decreases': [1118], 'MLC20.': [1122], 'cannot': [1125, 2619], 'rule': [1126, 1686], 'possibility': [1129, 1690, 2603], 'more': [1131, 2145], 'complicated': [1132], 'during': [1141], 'nonsteady': [1143], 'phases': [1145], 'relaxation.With': [1149], 'mind,': [1152], 'set': [1154], 'establish': [1157], 'whether': [1158, 1357, 1564], 'causes': [1160], 'inactivation': [1161], 'MLCK,': [1163, 1400, 1462], 'activation': [1164, 1482, 1652, 1737, 1831, 1858, 2571, 2795, 2821], 'MLCP,': [1166], 'combination': [1169], 'thereof.': [1170], 'It': [1171, 1813, 2317], 'known': [1174, 2518, 2642], 'vitro': [1177, 1278, 1891, 2054], 'site': [1183, 1224, 1282, 1886, 1909, 1917, 2078], 'protein': [1186, 1189, 1339, 1836, 1860, 2587, 2614], 'A,': [1188], 'C,': [1191], 'Ca2+-calmodulin-dependent': [1193], 'II': [1195], 'inactivates': [1196], '(20Stull': [1206], 'Hsu': [1208], 'L.-C.': [1209], 'Tansey': [1210], 'M.G.': [1211], 'Kamm': [1212, 2770], '1990;': [1217, 2598], '265:': [1218, 2599], '16683-16690Google': [1219], 'Hence': [1221], 'any': [1227, 1352], 'above': [1230, 1648], 'mentioned': [1231, 1647], 'kinases': [1232], 'would': [1233, 1798], 'lower': [1236, 1912, 1964], 'than': [1237, 1913, 2147], 'normal': [1238, 1807], 'level.': [1245], 'Not': [1246], 'surprisingly,': [1247], 'had': [1249, 1673], '(PKG)': [1256], 'phosphorylates': [1257], 'accounts': [1260], 'Direct': [1268], 'PKG': [1273, 1459, 1819, 2471, 2533, 2612], 'indeed': [1275, 1491], 'occur': [1276, 1923], 'A;': [1283], 'furthermore,': [1284], 'modify': [1289], '(21Nishikawa': [1294], 'Shirakawa': [1296], 'S.': [1297, 1612, 2007, 2107, 2760], 'Adelstein': [1298], 'R.S.': [1299], '1985;': [1303, 1317], '260:': [1304], '8978-8983Google': [1305], '22Hathaway': [1307], 'D.R.': [1308, 2256, 2414], 'Konicki': [1309], 'M.V.': [1310], 'Coolican': [1311], 'S.A.': [1312], 'Mol.': [1314], 'Cell.': [1315], 'Cardiol.': [1316], '17:': [1318], '841-850Google': [1319], 'Thus': [1321, 1774], 'biochemical': [1324, 1344], 'studies,': [1325], 'seems': [1327], 'capable': [1332], 'affecting': [1334], 'PKG.': [1341, 2576], 'isolated': [1347], 'useful': [1353], 'information': [1354], 'evaluate': [1356], 'an': [1358, 1466, 1722, 1833, 2298], 'indirect': [1359], 'occurred.': [1365], 'Our': [1366, 1645], 'experiment,': [1367], 'involving': [1369, 2514], 'virtually': [1370], 'complete': [1371], 'elimination': [1372], '4,': [1377], 'B)': [1380], 'lends': [1381], 'support': [1383], 'idea': [1386, 2507], 'unaffected': [1390], 'evidenced': [1402], 'ineffectiveness': [1405], 'rate': [1409, 1411, 1903, 1926, 2045, 2354, 2440, 2449], 'development': [1413, 2130], 'This': [1416, 2506], 'result': [1417], 'confirmed': [1419], 'two': [1421, 1428, 1445], 'different': [1422, 1430], 'types': [1423], 'permeabilized': [1425, 1550, 1993, 2276, 2482], 'preparations': [1426], 'using': [1427], 'structurally': [1429], 'selective': [1431], 'inhibitors.': [1433], 'We': [1434, 1855], 'realize': [1435], 'conceivable,': [1439], 'although': [1440], 'unlikely,': [1441], 'chosen': [1444], 'inhibitors': [1447], 'MC-LR': [1448], 'calyculin': [1450], 'somehow': [1452, 1697], 'interfere': [1453], 'putative': [1456, 1700], 'inhibit': [1461], 'thus': [1463], 'hiding': [1464], 'effect.At': [1467], 'point': [1469], 'now': [1471, 2819], 'seemed': [1472], 'default': [1474], 'exerting': [1478], 'experiment': [1486], 'summarized': [1487], 'Fig.': [1489, 1670, 1693, 1716, 2431], '5': [1490, 1671], 'indicates': [1492], 'mechanism;': [1495], 'relaxation.': [1504], 't1/2': [1506, 1520], '1.4': [1510], 'times': [1511, 1524], 'greater': [1512, 1570], 'absence': [1515], '1.3': [1523], 'greater.': [1525], 'One': [1526, 2544], 'concern': [1527], 'have,': [1529], 'over': [1532], 'inability': [1534], 'chelator': [1538], 'EGTA': [1539, 2163, 2372], 'inhibitor': [1543], 'ML-9': [1544, 2178, 2374], 'diffuse': [1546], 'instantaneously': [1547], 'into': [1548], 'abolish': [1554], 'immediately,': [1558], 'creating': [1559], 'curiosity': [1561], 'even': [1569, 2194], 'under': [1571, 1806, 1951], 'ideal,': [1572], 'diffusion-independent': [1573], 'conditions.An': [1574], 'alternative': [1575], 'approach': [1576], 'investigating': [1578], 'compare': [1584], 'experimental': [1589, 1709, 1778], 'courses': [1591], 'what': [1593], 'predicted': [1596], 'theoretical': [1599, 1711, 1873], '(23Kitazawa': [1601, 2096, 2749], 'Proc.': [1607, 2102, 2755], 'Natl.': [1608, 2103, 2756], 'Acad.': [1609, 2104, 2757], 'Sci.': [1610, 2105, 2758], 'U.': [1611, 2106, 2759], '88:': [1615, 2110, 2763], '9307-9310Google': [1616, 2111, 2764], '24Mitsui': [1618, 2113], 'Kitazawa': [1620, 2010, 2115], 'Ikebe': [1622, 2008, 2117], '269:': [1628, 2123], '5842-5848Google': [1629, 2124], 'based': [1633, 1881], 'simple': [1636], 'two-state': [1637], 'phosphorylation/dephosphorylation': [1638], 'reaction': [1639], '6;': [1641], 'see': [1643], 'below).': [1644], 'indicated': [1649], 'predominantly': [1653], 'accounted': [1654], 'suspected': [1664], 'diffusion': [1666, 2161], 'limitations': [1667], 'protocol': [1672, 1695], 'visibility': [1677], 'activation.': [1681], 'Additionally,': [1682], 'completely': [1688], '4': [1694], 'masking': [1698], '8Br-cGMP.': [1704], 'comparison': [1706], '6': [1717], 'addresses': [1718], 'those': [1719], 'concerns': [1720], 'ideological': [1723], 'manner': [1724, 1757], 'suggests': [1726], 'elicits': [1729], 'substantial': [1736], '3.8-fold': [1741], 'simulated': [1751], 'similar': [1759, 1925, 2132], 'comparable': [1761], 'actual': [1770], 'contractions.': [1773], 'conclude': [1776], 'creates': [1782], 'marked': [1783], 'activating': [1794], 'That': [1796], 'finding': [1797], 'imply': [1800], 'situ': [1805], 'conditions': [1809, 2095, 2200], 'maximal.': [1812], 'interestingly': [1816], 'suggested': [1817, 2288, 2347], 'pituitary': [1821], 'tumor': [1822], 'cells': [1823], 'stimulates': [1824], 'Ca2+-': [1825], 'voltage-activated': [1827], 'potassium': [1828], 'channels': [1829], 'okadaic': [1834], 'acid-sensitive': [1835], '(25White': [1838], 'R.E.': [1839], 'A.B.': [1841, 2677], 'Shcherbatko': [1842], 'A.D.': [1843], 'Lincoln': [1844], 'Schonbrunn': [1846], 'Armstrong': [1848], 'D.L.': [1849], '361:': [1852], '263-266Google': [1853], 'may': [1864, 2301, 2333], 'general': [1867], 'biological': [1870], 'systems.In': [1871], 'model,': [1874], 'made': [1876], 'predictions': [1877], 'regarding': [1878], 'single': [1884], 'MLCK.': [1888], 'Although': [1889], 'phosphorylate': [1894], '2': [1895], 'mol/mol': [1896, 1962, 1990], '(Ser-19': [1899], 'Thr-18),': [1901], 'much': [1911, 2144], 'former': [1916], 'their': [1919], 'dephosphorylations': [1920], '(26Ikebe': [1927, 2027], 'Hartshorne': [1929, 2029], 'Elzinga': [1931, 2031], '261:': [1937, 2037], '36-39Google': [1938, 2038], 'Scholar),': [1939, 2601, 2696, 2781], 'suggesting': [1940], 'small': [1945], 'portion': [1946], 'diphosphorylated': [1950], 'conditions.': [1953], 'fact,': [1955], 'extent': [1957], '1': [1961, 2170], '(27Barany': [1969], 'Barany': [1971, 1973, 2059], 'Muscle': [1978, 2064], 'Contraction.': [1979, 2065], 'Academic': [1980, 2066], 'Press': [1981, 2067], 'Inc.,': [1982, 2068], 'San': [1983, 2069], 'Diego,': [1984, 2070], 'CA1996:': [1985, 2071], '21-35Google': [1986], '1.1-1.2': [1989], 'maximally': [1995], 'stimulated': [1996], 'ions': [1999], 'alone': [2000], 'GTPγS': [2003], '(15Masuo': [2004], 'Reardon': [2006], 'Gen.': [2013], '104:': [2016], '265-286Google': [2017], 'Additional': [2019], 'Thr-18': [2020], 'increases': [2022, 2438], 'actin-activated': [2024], 'movement': [2047], 'actin': [2049], 'filaments': [2050], 'motility': [2055], 'assay': [2056], '(28Sellers': [2057], '181-190Google': [2072], 'Therefore,': [2074], 'enough': [2082], 'appropriate': [2084], 'simulate': [2086], 'MLC': [2136, 2167, 2338, 2442], '4),': [2139], 'occurred': [2143], 'slowly': [2146], '5).': [2151, 2207], 'were': [2157, 2201], 'somewhat': [2158], 'affected': [2159], 'ML-9,': [2165], 'min': [2171], 'Ca2+-free': [2174], '(EGTA': [2175], 'mM),': [2177], 'solution': [2179], '60%': [2183], 'control': [2185], '73%': [2188], '8Br-cGMP,': [2193], 'though': [2195], '3-4%': [2205], 'These': [2208], 'observations': [2209], 'consistent': [2211], 'previous': [2213, 2485], '(29Chatterjee': [2215], 'Murphy': [2217, 2238, 2689], 'R.A.': [2218, 2239, 2690], 'Science.': [2219], '1983;': [2220], '221:': [2221], '464-466Google': [2222], 'explained': [2227], 'slow': [2230], 'onset': [2231], 'detachment': [2232, 2353, 2448], 'dephosphorylated': [2234, 2315, 2356, 2451], 'cross-bridges': [2235, 2310], '(30Hai': [2236], 'C.-M.': [2237], '285-298Google': [2245], 'Recently,': [2247], 'Khromov': [2248, 2406], 'et': [2249, 2407, 2489], 'al.': [2250, 2408, 2490], '(31Khromov': [2251, 2409], 'Trentham': [2255, 2413], 'Zimmermann': [2257, 2415], 'B.': [2258, 2416], '69:': [2264, 2422], '2611-2622Google': [2265, 2423], 'precisely': [2267], 'analyzed': [2268], 'biphasic': [2270, 2380], 'photolysis': [2281], 'caged': [2284], 'chelator.': [2286], 'They': [2287], 'initial': [2291], 'plateau': [2292, 2324, 2389], 'phase': [2293, 2325, 2345, 2390], 'followed': [2296], 'exponential': [2299, 2343, 2400], 'decay': [2300, 2344], 'due': [2303], 'continued': [2305], 'cycling': [2306, 2313], 'remaining': [2308], 'phosphorylated': [2309], 'cooperative': [2312, 2662], 'cross-bridges.': [2316], 'therefore': [2319], 'length': [2321], 'tonic': [2329], 'depend': [2334], 'inversely': [2335], 'upon': [2336], 'rate.': [2340, 2364], 'representative': [2350], 'cross-bridges,': [2357], 'independent': [2360], 'initiated': [2370], '5A).': [2382], 'duration': [2386], 'subsequent': [2399], 'decay.': [2401], 'interpret': [2426], '5,': [2432], 'again': [2434], 'concludes': [2435], 'cross-bridge.The': [2452], 'loss': [2453], "8Br-cGMP's": [2455], 'heavily': [2459], 'demembranated': [2461], 'samples': [2462], 'implies': [2463], 'soluble': [2466], 'target(s),': [2468], 'diffusible': [2473], 'cofactor(s),': [2474], 'mediates': [2475], 'selectively': [2481], 'fibers,': [2483], 'confirming': [2484], 'Pfitzer': [2488], 'agreement': [2510], 'studies': [2513], 'independently': [2531], '(9Lincoln': [2534], 'then': [2546], 'reasonably': [2547], 'assume': [2548], 'low': [2551], 'IC50': [2553], 'unknown': [2562], 'anticipatory': [2563], 'target': [2564], 'coupling': [2567], 'likely': [2575], 'activate': [2585], 'cAMP-dependent': [2586], '(32Shabb': [2589], 'J.B.': [2590], 'Ng': [2591], 'Corbin': [2593], 'J.D.': [2594], '16031-16034Google': [2600], 'micromolar': [2606], 'stimulate': [2610], 'signaling': [2617], 'excluded.The': [2621], 'determinant': [2630], 'hence': [2637], 'contraction.': [2640], 'tissue': [2646], 'handling': [2648], 'cytoplasmic': [2649], 'modulating': [2653], 'synergistic': [2660], 'pathways.': [2663], 'Excitatory': [2664], 'agonists': [2665, 2741, 2811], 'elevating': [2669], 'increasing': [2673], '(33Bradley': [2676], '1987;': [2683], '385:': [2684], '437-448Google': [2685], '34Rembold': [2687], 'C.M.': [2688], 'Circ.': [2691], '1988;': [2693, 2721, 2734], '63:': [2694], '593-603Google': [2695], 'whereas': [2697, 2782], 'substances': [2699], 'reducing': [2703], '(35Karaki': [2710], 'H.': [2711, 2715], 'Sato': [2712], 'Ozaki': [2714], 'Murakami': [2716], 'Eur.': [2718], 'Pharmacol.': [2720, 2733], '156:': [2722], '259-266Google': [2723], '36Yanagisawa': [2725], 'Kawada': [2727], 'Taira': [2729], 'N.': [2730], 'Br.': [2731], '98:': [2735], '469-482Google': [2736], 'Furthermore,': [2738], 'excitatory': [2740, 2810], 'sensitization': [2744], '37Kubota': [2766], 'Nomura': [2768], 'Mumby': [2772], 'M.C.': [2773], 'Am.': [2776], '1992;': [2779], '262Google': [2780], 'Thus,': [2798], 'endothelium': [2802], 'production': [2804], 'NO': [2806], 'antagonize': [2809], 'almost': [2813], 'every': [2814], 'reported': [2815], 'mechanism,': [2816], 'including': [2817], 'apparent': [2820], 'versus': [2824], 'agonist-induced': [2825]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1972331465', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 6}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 5}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 6}, {'year': 2016, 'cited_by_count': 5}, {'year': 2015, 'cited_by_count': 9}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 8}, {'year': 2012, 'cited_by_count': 8}], 'updated_date': '2024-12-14T07:43:29.808616', 'created_date': '2016-06-24'}