Title: Alteration of proline hydroxylase activity by glucocorticoids
Abstract: The biochemical mechanism by which glucocorticoids affect collagen metabolism was investigated. Triamcinolone, hydrocortisone and methylprednisolone significantly decreased liver proline hydroxylase activity in vivo to an extent unrelated to the decrease of protein in the 15,000 g supernatant. Triamcinolone inhibited liver proline hydroxylase in a dose-dependent manner in vivo. An observed elevation of liver proline hydroxylase activity in adrenalectomized animals was decreased to control levels by hydrocortisone treatment. Subdermal implants of polyvinyl sponges were used to induce granuloma growth as a model system of inflammation. The anti-inflammatory activity of corticosteroids was correlated with inhibition of proline hydroxylase activity in granuloma tissue. The data indicate that corticosteroids decrease collagen synthesis by inhibiting the proposed rate-limiting enzyme. This inhibitory effect on collagen metabolism is correlated with observed anti-inflammatory activity.
Publication Year: 1971
Publication Date: 1971-10-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 35
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