Publication Information

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'https://openalex.org/W2073751661', 'https://openalex.org/W2077053926', 'https://openalex.org/W2086660575', 'https://openalex.org/W2091177185', 'https://openalex.org/W2091593313', 'https://openalex.org/W2100837269', 'https://openalex.org/W2106357210', 'https://openalex.org/W2111817213', 'https://openalex.org/W2148404862', 'https://openalex.org/W2314225431', 'https://openalex.org/W2320556745', 'https://openalex.org/W2888511473', 'https://openalex.org/W652937571'], 'related_works': ['https://openalex.org/W2084176726', 'https://openalex.org/W2074626316', 'https://openalex.org/W2066721020', 'https://openalex.org/W2030300189', 'https://openalex.org/W2005264953', 'https://openalex.org/W1997717245', 'https://openalex.org/W1985929559', 'https://openalex.org/W1974264311', 'https://openalex.org/W1972067409', 'https://openalex.org/W1966123072'], 'abstract_inverted_index': {'The': [0, 30, 101, 191, 221, 292, 500, 536, 560, 620, 687, 1078, 1096, 1119, 1432, 1487, 1519, 1701, 1755, 1800, 1925, 2107, 2246, 2303, 2490, 2570, 2638, 2653, 2752, 2756, 2766, 2775, 2796, 2880, 3023, 3113, 3128, 3352, 3496, 3557, 3572, 3587, 3604, 3797, 3867, 4057, 4151, 4315, 4327, 4384], 'epitope': [1, 74, 192, 265, 777, 909, 953, 1337, 1346, 2868, 3453, 3700, 3749], 'recognized': [2, 193, 780, 954, 972, 2839, 3034, 3177, 3918], 'by': [3, 23, 194, 214, 615, 781, 940, 955, 973, 1060, 1084, 1328, 1368, 1447, 1473, 1496, 1557, 1676, 1680, 1709, 1722, 2177, 2262, 2293, 2318, 2327, 2399, 2434, 2475, 2579, 2670, 2700, 2728, 2840, 2857, 2900, 2922, 2990, 3035, 3178, 3240, 3493, 3617, 3780, 3834, 3911, 3919, 4170, 4177, 4278, 4292, 4324, 4411, 4416, 4421], 'a': [4, 61, 109, 154, 195, 252, 300, 345, 408, 419, 616, 794, 797, 801, 831, 838, 1088, 1508, 1719, 1767, 1812, 1824, 1837, 1843, 1883, 1976, 1996, 2005, 2047, 2159, 2166, 2186, 2266, 2362, 2476, 2537, 2600, 2632, 2671, 2701, 2739, 2845, 2907, 2953, 3219, 3222, 3361, 3404, 3463, 3476, 3531, 3560, 3567, 3577, 3597, 3688, 3707, 3736, 3885, 4064, 4096, 4127, 4173, 4321, 4375, 4408, 4417, 4429], 'monoclonal': [5, 196, 387, 411, 728, 3406], 'antibody': [6, 197, 2527, 2572, 3268, 3407, 4112], '(mAb19)': [7, 198], 'directed': [8, 199, 413, 3269], 'against': [9, 200, 414, 3270], 'the': [10, 27, 40, 49, 68, 73, 136, 139, 142, 164, 170, 172, 176, 183, 188, 201, 218, 231, 240, 259, 264, 327, 330, 333, 355, 361, 363, 367, 374, 379, 394, 425, 451, 457, 476, 533, 543, 556, 565, 590, 595, 603, 609, 624, 655, 663, 676, 740, 775, 787, 827, 834, 863, 868, 877, 887, 905, 908, 911, 914, 921, 933, 936, 952, 965, 969, 981, 992, 1019, 1050, 1123, 1129, 1136, 1140, 1162, 1236, 1242, 1271, 1274, 1345, 1350, 1354, 1420, 1427, 1448, 1491, 1526, 1554, 1593, 1597, 1601, 1607, 1634, 1641, 1645, 1649, 1652, 1656, 1660, 1670, 1682, 1685, 1694, 1698, 1705, 1712, 1760, 1785, 1789, 1794, 1803, 1809, 1820, 1829, 1834, 1849, 1852, 1873, 1913, 1942, 1969, 1984, 1987, 2008, 2032, 2122, 2125, 2129, 2146, 2172, 2180, 2196, 2199, 2207, 2215, 2253, 2350, 2353, 2356, 2408, 2431, 2451, 2458, 2481, 2504, 2509, 2513, 2517, 2523, 2534, 2540, 2559, 2563, 2583, 2588, 2592, 2604, 2609, 2619, 2625, 2641, 2782, 2799, 2810, 2815, 2819, 2824, 2827, 2854, 2867, 2872, 2877, 2885, 2896, 2904, 2915, 2928, 2960, 2968, 2979, 2985, 3031, 3044, 3060, 3070, 3077, 3106, 3125, 3134, 3138, 3143, 3165, 3172, 3182, 3187, 3190, 3197, 3211, 3216, 3231, 3234, 3249, 3252, 3271, 3275, 3300, 3306, 3309, 3317, 3321, 3326, 3329, 3336, 3385, 3410, 3414, 3421, 3446, 3452, 3473, 3482, 3534, 3563, 3583, 3618, 3627, 3632, 3643, 3653, 3661, 3670, 3675, 3691, 3699, 3723, 3727, 3748, 3764, 3808, 3836, 3841, 3849, 3874, 3901, 3905, 3922, 3929, 3933, 3937, 3948, 3993, 4005, 4010, 4030, 4049, 4054, 4074, 4110, 4160, 4204, 4214, 4218, 4257, 4265, 4271, 4281, 4285, 4333, 4352, 4360, 4363, 4369, 4380, 4392, 4398, 4424, 4433, 4436, 4442, 4452, 4467, 4470, 4474], 'β2': [11, 80, 202, 271, 458, 468, 558, 561, 607, 698, 993, 1036, 1099, 1124, 2468, 2598, 3167, 3176, 3310], 'subunit': [12, 203, 494, 562, 1037, 1125], 'of': [13, 26, 32, 36, 51, 58, 70, 72, 79, 138, 169, 175, 204, 217, 223, 227, 242, 249, 261, 263, 270, 329, 360, 366, 403, 410, 421, 428, 448, 480, 495, 502, 539, 567, 592, 606, 623, 654, 679, 691, 744, 786, 796, 804, 865, 876, 880, 884, 907, 926, 935, 991, 1038, 1098, 1101, 1113, 1122, 1142, 1230, 1245, 1347, 1356, 1390, 1402, 1414, 1443, 1490, 1525, 1538, 1592, 1606, 1636, 1640, 1651, 1697, 1704, 1711, 1759, 1802, 1811, 1819, 1836, 1842, 1912, 1915, 1968, 1986, 2050, 2060, 2064, 2070, 2077, 2083, 2091, 2098, 2105, 2124, 2131, 2179, 2189, 2195, 2198, 2206, 2259, 2352, 2368, 2454, 2463, 2492, 2516, 2525, 2543, 2557, 2603, 2606, 2622, 2635, 2655, 2798, 2818, 2826, 2869, 2876, 2884, 2903, 2973, 3043, 3063, 3124, 3145, 3175, 3189, 3203, 3233, 3274, 3308, 3316, 3413, 3448, 3451, 3475, 3569, 3620, 3652, 3679, 3722, 3800, 3838, 3904, 4009, 4048, 4051, 4073, 4082, 4089, 4103, 4106, 4126, 4129, 4162, 4267, 4280, 4329, 4351, 4362, 4374, 4379, 4423, 4428, 4435, 4469], 'Escherichia': [14, 205, 481, 496], 'coli': [15, 206, 482, 497, 667, 1040, 1714], 'tryptophan': [16, 207, 483, 498, 540, 610, 1041, 1132, 1608, 3630], 'synthase': [17, 208, 541, 611, 1042, 1609], 'was': [18, 99, 104, 209, 290, 295, 456, 772, 778, 872, 910, 1002, 1021, 1043, 1082, 1126, 1233, 1277, 1302, 1339, 1397, 1423, 1434, 1494, 1505, 1631, 1663, 1707, 1763, 1927, 1973, 2013, 2019, 2039, 2109, 2148, 2211, 2271, 2306, 2359, 2473, 2530, 2549, 2576, 2615, 2644, 2697, 2754, 2760, 2769, 2778, 2785, 2802, 2851, 2888, 2925, 2963, 3047, 3228, 3238, 3530, 3559, 3716, 3778, 3831, 3880, 3892, 3908, 3917, 4061, 4116, 4168, 4276, 4382, 4387, 4401], 'found': [19, 210, 2020, 3163, 3935], 'to': [20, 45, 65, 106, 211, 236, 256, 297, 385, 392, 532, 695, 733, 874, 928, 946, 1131, 1765, 1783, 1792, 1858, 1982, 2021, 2111, 2120, 2142, 2154, 2226, 2324, 2417, 2443, 2457, 2522, 2617, 2813, 2822, 2891, 2937, 3001, 3041, 3069, 3121, 3196, 3358, 3609, 3625, 3645, 3693, 3703, 3706, 3718, 3735, 3954, 4018, 4108, 4133, 4157, 4320, 4358, 4463], 'be': [21, 132, 212, 323, 548, 684, 875, 2022, 3522, 3626, 3658, 3683, 3704, 4036, 4414, 4464], 'carried': [22, 213, 2373], 'residues': [24, 96, 215, 287, 966, 2863, 2875, 3122, 3226, 3570, 3648, 3984], '2-9': [25, 41, 216, 232, 1653, 1795, 1821, 3123, 3139, 3183, 3227, 3253, 3301, 3322, 3337, 3386, 3422, 3584, 3949, 3985], 'β': [28, 93, 177, 219, 284, 368, 693, 1610, 1658, 2828, 2878, 2886, 3126, 3223, 3276, 3318, 3330, 3383, 3415, 3419, 3654, 3680], 'chain.': [29, 220, 2879, 3127, 3416], 'affinities': [31, 222, 2995, 3153, 3837, 3938], 'mAb19': [33, 52, 224, 243, 782, 866, 885, 974, 1275, 2404, 2841, 2870, 2949, 2997, 3036, 3056, 3179, 3665, 3774, 3839, 3920, 4028, 4052, 4107, 4134, 4215], 'for': [34, 53, 67, 225, 244, 258, 424, 867, 886, 943, 1342, 1542, 1600, 1633, 1937, 2015, 2025, 2029, 2036, 2113, 2174, 2297, 2332, 2551, 2640, 2725, 2965, 2996, 3132, 3164, 3305, 3840, 3936, 3947, 3958, 4053, 4118, 4142, 4203, 4451, 4479], 'peptides': [35, 226, 1492, 1527, 2132, 2210, 2260, 2285, 2316, 2397, 2432, 2838, 2898, 2982, 3136, 3725, 3741, 3751, 3809, 3842, 3995, 4012], 'different': [37, 228, 415, 1772, 1804, 1878], 'lengths': [38, 229], 'containing': [39, 230, 1669, 1684, 2355, 3137, 3674], 'sequence': [42, 174, 233, 365, 660, 1352, 1598, 1860, 2370, 2920, 2962, 3116, 3140, 3272, 3302, 3411, 3535, 3678], 'were': [43, 234, 1058, 1103, 1184, 1364, 1417, 1445, 1471, 1528, 1551, 1856, 1880, 1917, 1993, 2044, 2140, 2169, 2251, 2286, 2322, 2372, 2386, 2401, 2421, 2446, 2667, 2736, 2842, 2988, 2998, 3141, 3339, 3356, 3491, 3499, 3527, 3590, 3607, 3732, 3755, 3939, 4077, 4139, 4154, 4220, 4288, 4318], 'close': [44, 235, 3040, 3953], '0.6': [46, 237], '×': [47, 238, 2547, 3147, 3161, 3882, 3890, 4229, 4234, 4239, 4244, 4249, 4254], '109M−,': [48, 239], 'affinity': [50, 241, 864, 1268, 3039, 3129, 3868, 4050, 4480], 'native': [54, 245, 697, 888, 922, 1035, 3045, 3107, 3166, 3959], 'β2.': [55, 116, 246, 307], 'In': [56, 135, 247, 326, 725, 2118], 'view': [57, 248], 'these': [59, 250, 449, 2858], 'results,': [60, 251], 'model': [62, 253, 452], 'is': [63, 254, 542, 599, 613, 672, 836, 919, 2520, 3073, 3084, 3303], 'proposed': [64, 255], 'account': [66, 257], 'kinetics': [69, 260], 'appearance': [71, 262], 'during': [75, 266, 739], 'in': [76, 163, 182, 187, 267, 354, 373, 378, 509, 564, 608, 688, 741, 913, 920, 930, 968, 980, 1128, 1135, 1139, 1161, 1228, 1399, 1426, 1553, 1644, 1655, 1808, 1833, 1862, 1886, 1938, 1980, 1999, 2046, 2134, 2158, 2202, 2263, 2313, 2319, 2375, 2435, 2470, 2507, 2512, 2528, 2574, 2608, 2624, 2729, 2738, 2861, 2984, 3026, 3074, 3087, 3142, 3181, 3210, 3342, 3434, 3596, 3660, 3698, 3757, 3776, 3805, 3846, 3877, 3914, 3921, 3928, 3992, 3997, 4000, 4024, 4038, 4063, 4079, 4165, 4172, 4217, 4261, 4270, 4273, 4391], 'vitro': [77, 268, 689, 742, 1939, 2000, 2264, 2320, 2436, 2730, 3758, 4001], 'renaturation': [78, 269, 743], '(Murry-Brelier,': [81, 272], 'A.,': [82, 273], 'and': [83, 131, 152, 167, 186, 274, 322, 343, 358, 377, 400, 417, 466, 471, 475, 555, 582, 668, 790, 942, 975, 988, 997, 1013, 1032, 1045, 1049, 1054, 1100, 1115, 1160, 1201, 1325, 1335, 1349, 1419, 1440, 1476, 1483, 1639, 1672, 1690, 1780, 1788, 1828, 1869, 1872, 1923, 1934, 2018, 2102, 2127, 2152, 2183, 2228, 2365, 2423, 2448, 2763, 2781, 2807, 2848, 2895, 2917, 2950, 2971, 2993, 3185, 3201, 3230, 3350, 3366, 3372, 3511, 3519, 3592, 3611, 3650, 3744, 3770, 3815, 3823, 3844, 3884, 3951, 4100, 4123, 4135, 4149, 4159, 4284], 'Goldberg,': [84, 275], 'M.': [85, 276, 3091], 'E.(1988)': [86, 277], 'Biochemistry': [87, 278], '27,': [88, 279], '7633-7640).': [89, 280], 'A': [90, 281, 1590, 1896, 1952, 2053, 2611, 4275], 'mutant': [91, 282], 'producing': [92, 283], 'chains': [94, 128, 148, 285, 319, 339, 554, 694, 3506], 'lacking': [95, 286, 3225], '1-9': [97, 140, 173, 288, 331, 364, 4353, 4367], '(βΔ1–9)': [98, 289], 'prepared.': [100, 291], 'βΔ1–9': [102, 118, 143, 293, 309, 334], 'protein': [103, 144, 294, 335, 453, 835, 3046, 3108, 3237, 3250, 3311, 3464, 3601, 4068], 'able': [105, 296], 'fold': [107, 298], 'into': [108, 299, 551], 'heat': [110, 301, 1061], 'stable': [111, 302], 'homodimer': [112, 303], 'resembling': [113, 304], 'wild': [114, 305, 2464, 3390, 3426], 'type': [115, 306, 2465, 2567, 3391, 3427], 'Isolated': [117, 308], 'had': [119, 310, 2860, 3255], 'no': [120, 311, 673, 3256, 4412], 'detectable': [121, 312, 2939, 3257], 'enzymatic': [122, 189, 313, 380, 989, 1120], 'activity.': [123, 190, 314, 381], 'It': [124, 315, 771, 924], 'could': [125, 145, 316, 336, 3054, 3656, 3681, 4410], 'bind': [126, 146, 317, 337, 3055], 'α': [127, 147, 318, 338, 553, 593, 986, 1143], 'extremely': [129, 320, 685], 'weakly': [130, 321], 'slightly': [133, 324, 3156, 3941], 'activated.': [134, 325], 'presence': [137, 328, 566, 591, 1137, 3619], 'peptide,': [141, 332], 'much': [149, 340, 4475], 'more': [150, 341, 931, 2976], 'strongly': [151, 180, 342, 371, 3057, 3169, 3264, 3827], 'generate': [153, 344, 2814], '50%': [155, 346], 'active': [156, 347], 'enzyme.': [157, 348], 'Thus,': [158, 349, 3685, 3899, 4004], 'although': [159, 350, 3080], 'having': [160, 351], 'little': [161, 352], 'role': [162, 353, 979], 'overall': [165, 356], 'folding': [166, 357, 401, 737, 788, 798, 828, 870, 937], 'stability': [168, 359], 'protein,': [171, 362], 'chain': [178, 369, 2829, 2887, 3192, 3224, 3319, 3655], 'appears': [179, 370], 'involved': [181, 372, 967], 'α-β': [184, 375], 'interactions': [185, 376], 'INTRODUCTIONIn': [382], 'an': [383, 735, 1466, 1535, 1863, 2193, 2376, 2389, 3038, 3267, 3621, 3719, 3989, 4022, 4356], 'attempt': [384, 4357], 'use': [386, 4279], 'antibodies': [388, 412, 1183, 2415, 2441], 'as': [389, 793, 882, 917, 948, 950, 1063, 1145, 1222, 1263, 1279, 1359, 1361, 1366, 1718, 1882, 2405, 2645, 2927, 3368, 3594, 3666, 3759, 3768, 3925, 3927, 4175, 4221, 4290], 'conformational': [390, 396, 398], 'probes': [391], 'investigate': [393], 'structure,': [395], 'changes,': [397], 'dynamics,': [399], 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4310], 'Scopus': [442, 721, 754, 767, 817, 858, 898, 1074, 1156, 1179, 1197, 1218, 1296, 1321, 1384, 1459, 1572, 1586, 1627, 1737, 1751, 2242, 2279, 2342, 2690, 2721, 3019, 3102, 3293, 3553, 3977, 4196, 4311], '(75)': [443], 'Google': [444, 521, 651, 723, 756, 769, 819, 860, 900, 1076, 1158, 1181, 1199, 1220, 1261, 1298, 1323, 1386, 1461, 1574, 1588, 1629, 1739, 1753, 2244, 2281, 2344, 2692, 2723, 3021, 3104, 3295, 3555, 3795, 3865, 3979, 4198, 4313], 'Scholar).': [445, 522, 652, 724, 770, 820, 861, 901, 1077, 1589, 1754, 1909, 1965, 2245, 2502, 3022, 3296, 3556, 3796, 3866, 3980, 4199, 4314], 'For': [446, 1267, 1648, 2407, 2430], 'most': [447], 'studies,': [450], 'used': [455, 461, 1001, 1632, 1662, 1717, 1764, 1936, 2250, 2305, 2422, 2447, 2616, 3593, 4478], '1The': [459], 'abbreviations': [460], 'are:': [462], 'β2,': [463], 'F1,': [464], 'F2,': [465], 'β,': [467], 'subunitthe': [469], 'N-': [470], 'C-terminal': [472, 1055], 'proteolytic': [473], 'domains,': [474], 'polypeptide': [477, 1300, 3191, 3505, 4430], 'chain,': [478, 1659, 3331], 'respectively,': [479], 'synthaseELISAenzyme-linked': [484], 'immunosorbent': [485], 'assaymAbmonoclonal': [486], 'antibodyPAGEpolyacrylamide': [487], 'gel': [488, 1085, 1474, 1979, 2147, 2629, 2905, 2916, 4205], 'electrophoresisRIAradioimmunoassayCHAPS3-cyclohexylamino-1-propanesulfonic': [489], 'acidHPLChigh': [490], 'performance': [491], 'liquid': [492], 'chromatography.': [493], 'synthase.': [499], 'properties': [501], 'this': [503, 956, 2544, 3713, 3801, 4330], 'enzyme': [504, 625, 2561, 2607, 2623], 'been': [506, 630, 701], 'recently': [507], 'reviewed': [508], 'detail(2Miles': [510], 'E.W.': [511, 638], 'Adv.': [512], 'Enzymol.': [513, 1567, 1732], 'Relat.': [514], 'Areas': [515], 'Mol.': [516, 1315, 3287, 3547, 3971], 'Biol.': [517, 642, 1316, 3548, 3972], '64:': [519], '93-172PubMed': [520], 'We': [523], 'shall': [524], 'summarize': [525], 'here': [526], 'those': [527], 'which': [528, 546, 2037, 2145, 2508, 2865, 3730], 'are': [529, 3154, 4045, 4449], 'directly': [530], 'relevant': [531], 'present': [534, 958], 'study.': [535], 'natural': [537], 'form': [538], 'heterotetramer': [544], 'α2β2,': [545], 'can': [547], 'readily': [549], 'dissociated': [550], 'monomeric': [552], 'dimeric': [557], 'subunit.': [559], 'can,': [563], 'its': [568, 1362, 2912, 2918, 3081], 'coenzyme': [569], 'pyridoxal-5′-phosphate,': [570], 'catalyze': [571], 'two': [572], 'distinct': [573], 'reactions:': [574], 'indole': [575, 1130], '+': [576, 580, 584, 588], 'L-serine': [577, 583], '→': [578, 586], 'L-tryptophan': [579], 'H2O': [581, 585], 'pyruvate': [587], 'ammonia.In': [589], 'chains,': [594, 987], 'serine': [596, 1163], 'deaminase': [597], 'reaction': [598, 612, 823, 2055, 2940], 'completely': [600], 'abolished,': [601], 'while': [602, 2943, 3261, 3900], 'specific': [604, 776, 1107, 1264, 3405], 'activity': [605, 990, 1121], 'increased': [614, 4440], 'factor': [617], 'about': [618, 2164], '30.': [619], 'three-dimensional': [621, 677], 'structure': [622], 'from': [626, 665, 1203, 1235, 1530, 1665, 1846, 1941, 2252, 2791, 2809, 2844, 2911, 3642, 3873, 4021], 'Salmonella': [627], 'typhimurium': [628], 'has': [629, 700], 'solved(3Hyde': [631], 'C.C.': [632], 'Ahmed': [633], 'S.A.': [634], 'Padlan': [635], 'E.A.': [636], 'Miles': [637], 'Davies': [639], 'D.R.': [640], 'J.': [641, 1168, 1289, 1314, 1377, 1452, 1577, 1579, 1742, 1744, 1889, 1945, 2683, 3012, 3546, 3970], 'Chem.': [643, 1454], '1988;': [644, 750, 813, 894], '263:': [645, 718, 855], '17857-17871Abstract': [646], 'Because': [653], 'very': [656, 3886, 3952], 'strong': [657], 'amino': [658, 1604, 2078, 2085, 2384], 'acid': [659, 1503, 2079, 2086], 'homology': [661], 'between': [662, 2761, 3647], 'enzymes': [664, 681], 'E.': [666, 1039, 1713], 'S.': [669, 714, 759, 851, 1174], 'typhimurium,': [670], 'there': [671], 'doubt': [674], 'that': [675, 774, 883, 904, 944, 1818, 2030, 2830, 2959, 3030, 3042, 3059, 3159, 3171, 3186, 3248, 3299, 3408, 3465, 3504, 3639, 3739, 3745, 3944, 3955, 3983, 4033, 4447], 'structures': [678, 4427], 'both': [680], 'also': [682, 3053], 'must': [683], 'similar.': [686], 'refolding': [690], 'denatured': [692], 'regenerate': [696], 'subunits': [699, 2469], 'extensively': [702], 'investigated(4Goldberg': [703], 'Semisotnov': [705, 842], 'G.V.': [706, 843], 'Friguet': [707, 844, 1188, 1306, 1369, 2486, 2649, 3002, 3281, 3538, 3962, 4178, 4293], 'B.': [708, 845, 1189, 1207, 1282, 1307, 1372, 1612, 2487, 2650, 2676, 2706, 3005, 3282, 3539, 3963, 4181, 4296], 'Kuwajima': [709, 846], 'K.': [710, 847, 1249, 3783, 3853], 'Ptitsyn': [711, 848], 'O.B.': [712, 849], 'Sugai': [713, 850], 'FEBS': [715, 852], 'Lett.': [716, 853], '1990;': [717, 763, 854], '51-56Crossref': [719, 856], '(100)': [722, 859], 'particular,': [726], 'one': [727], 'antibody,': [729], 'mAb19,': [730, 941, 1348, 2832, 2942, 3260], 'turned': [731], 'out': [732, 2374, 3711], 'detect': [734, 2618, 3694], 'early': [736, 784, 825, 869, 915], 'step': [738, 799, 2515], 'β2(5Murry-Brelier': [745], 'A.': [746, 809, 890, 1066, 1175, 1618, 2712, 4187, 4302], 'Goldberg': [747, 760, 810, 891, 1067, 1190, 1210, 1287, 1312, 1619, 2681, 2713, 3010, 3092, 3285, 3544, 3968, 4188, 4303], 'Biochemistry.': [749, 762, 812, 893, 1069, 1152, 1192], '27:': [751, 814, 895], '7633-7640Crossref': [752, 815, 896], '(22)': [755, 818, 899], 'Scholar,': [757, 1575, 1740], '6Blond-Elguindi': [758], '29:': [764], '2409-2417Crossref': [765], '(42)': [768], 'shown': [773, 873, 3025, 3876], 'not': [779, 2934, 2951, 3067, 3194, 3456, 3657, 3909, 3987], 'at': [783, 807, 830, 963, 976, 1007, 1110, 1238, 1353, 1429, 1485, 1522, 1544, 1693, 1770, 2116, 2300, 2310, 2329, 2536, 2772, 3488, 3501, 3508, 3513, 3812, 3817, 4121, 4264, 4473], 'stages': [785], 'process': [789], 'became': [791], 'immunoreactive': [792, 3115, 3460, 3585, 3696, 4364], 'result': [795, 4019], 'with': [800, 985, 1047, 1093, 1270, 1438, 1688, 1851, 1919, 1929, 2185, 2214, 2347, 2361, 2388, 2403, 2425, 2450, 2554, 2587, 2591, 2596, 2941, 2948, 2952, 3037, 3076, 3111, 3205, 3243, 3265, 3346, 3379, 3403, 3485, 3566, 3664, 3726, 3753, 3763, 3773, 3828, 3870, 3894, 4027, 4086, 4137, 4145, 4206, 4441], 'kinetic': [802], 'constant': [803, 4336], '0.06': [805], 's−': [806], '12°C(5Murry-Brelier': [808], 'This': [821, 902, 2931, 3072, 3297, 3830, 3981, 4114, 4445], 'intramolecular': [822], 'occurs': [824], 'on': [826, 1087, 1507, 1975, 2480, 2914, 3360, 4015, 4029, 4095], 'pathway,': [829], 'stage': [832], 'where': [833, 4397], 'still': [837], 'molten': [839], 'globule(4Goldberg': [840], 'Furthermore,': [862], 'intermediate': [871, 916], 'same': [878, 912], 'order': [879, 1981, 2119, 2821], 'magnitude': [881], 'protein(5Murry-Brelier': [889], 'suggested': [903, 2958, 4446], 'conformation': [906, 3738, 3991, 4361, 4419, 4468], 'it': [918, 1692, 2038, 3262, 3916], 'protein.': [923], 'seemed': [925, 3702, 4017], 'interest': [927, 1916], 'understand': [929], 'detail': [932], 'nature': [934], 'step(s)': [938], 'detected': [939, 3659, 3910], 'purpose': [945], 'characterize': [947, 4359], 'precisely': [949, 2977], 'possible': [951], 'antibody.The': [957], 'report': [959], 'describes': [960], 'experiments': [961], 'aimed': [962], 'identifying': [964], 'antigenic': [970, 3173, 3902, 4366], 'site': [971, 1696], 'assessing': [977], 'their': [978, 1531, 2849, 2994, 3771, 4025], 'folding,': [982], 'stability,': [983], 'association': [984], 'subunit.MATERIALS': [994], 'AND': [995], 'METHODSReagents': [996], 'BufferThe': [998], 'standard': [999, 1094, 1243, 2593, 4083], 'buffer': [1000, 1020, 2304, 4084], '0.1': [1003, 4376], 'M': [1004, 1024, 1028, 2072], 'potassium': [1005, 2010, 2073], 'phosphate': [1006], 'pH': [1008, 1030, 1545, 2311], '7.8,': [1009], '2': [1010, 1910, 2075, 2333, 2773, 3879, 4071], 'mM': [1011, 1015, 2024, 2082, 2308], 'EDTA,': [1012], '5': [1014, 4143], '2-mercaptoethanol.For': [1016], 'Western': [1017, 3241, 3377, 3401, 3612, 3662, 3912, 4040], 'blots,': [1018, 4031], 'TBS': [1022], '(0.05': [1023], 'Tris': [1025], 'base,': [1026], '0.15': [1027], 'NaCl,': [1029], '7.2).Protein': [1031], 'Proteolytic': [1033], 'FragmentThe': [1034], 'cleaved': [1046, 3085], 'trypsin,': [1048], 'resulting': [1051, 1421, 2897], 'N-terminal': [1052, 2369, 2881, 2919, 3061, 3082, 3644, 3677], 'F1': [1053, 1080, 1102, 1393, 2882], 'F2': [1056], 'fragments': [1057, 3640, 3673, 3697], 'separated': [1059], 'denaturation': [1062], 'described': [1064, 1146, 1223, 1280, 1367, 2646, 4176, 4291], 'previously(7Högberg-Raibaud': [1065], '1977;': [1070], '4014-4020Crossref': [1072], '(104)': [1075], 'solubilized': [1079], 'fragment': [1081, 1394, 1683, 2035, 2176, 2883, 3565, 3580, 3895, 3907, 3924, 4055, 4164, 4207, 4268], 'purified': [1083, 1202, 1472], 'filtration': [1086, 1475], 'S-200HR': [1089], 'Sephacryl': [1090], 'column': [1091], 'equilibrated': [1092], 'buffer.': [1095], 'concentrations': [1097, 4216, 4477], 'determined': [1104, 1365, 1506, 2014, 2578, 2921, 4169], 'spectrophotometrically': [1105], 'using': [1106, 1241, 1465, 1534, 1797, 1867, 1995, 2219, 2631, 2745, 3761, 3848], 'extinction': [1108, 1265, 1520, 1536], 'coefficients': [1109, 1521], '280': [1111, 1239, 1523], 'nm': [1112, 1524, 4396], '0.58': [1114], '0.67': [1116], 'cm2/mg,': [1117], 'respectively.': [1118], 'measured': [1127, 2999], 'reaction,': [1133], 'either': [1134, 2213, 2558, 2669, 3332], 'or': [1138, 1549, 1782, 1885, 2224, 2466, 2562, 2568, 2699, 2747, 3207, 3334, 3734, 4420], 'absence': [1141], 'subunit,': [1144, 3168], 'previously': [1147, 2483, 3957], '(8Faeder': [1148], 'E.J.': [1149], 'Hammes': [1150], 'G.G.': [1151], '1970;': [1153, 2275], '25:': [1154], '4043-4049Crossref': [1155], '(80)': [1157], 'Scholar),': [1159, 1630], 'deamination': [1164], 'reaction(9Crawford': [1165], 'I.P.': [1166, 3089], 'Ito': [1167], 'Proc.': [1169], 'Natl.': [1170], 'Acad.': [1171], 'U.': [1173], '1964;': [1176], '51:': [1177], '391-397Crossref': [1178], '(47)': [1180], 'Scholar).Monoclonal': [1182], 'obtained': [1185, 2398, 2511, 2586, 2595, 2627, 3131, 3634, 3869, 3893, 3996, 4277, 4287, 4386], '(10Djavadi-Ohaniance': [1186], 'L.': [1187, 1209, 1286, 1309, 1376, 2485, 2648, 2680, 3009, 3280, 3541, 3965], '1984;': [1193], '23:': [1194], '97-104Crossref': [1195], '(57)': [1198], 'Scholar)': [1200, 1221, 1262, 1324, 1462, 2693, 2724, 3105], 'ascitic': [1204], 'fluids': [1205], '(11Friguet': [1206], 'Djavadi-Ohaniance': [1208, 1285, 1308, 1375, 2679, 3008, 3279, 3540, 3964], 'Res.': [1212, 3096], 'Immunol.': [1213, 1290, 1378, 2684, 3013, 3288], '1989;': [1214], '140:': [1215], '355-376Crossref': [1216], '(43)': [1219], 'earlier.': [1224], 'Their': [1225], 'concentration,': [1226], 'expressed': [1227], 'terms': [1229], 'binding': [1231], 'sites,': [1232], 'estimated': [1234, 1529], 'absorbance': [1237, 2510], 'nm,': [1240], 'value': [1244, 3888], '1.5': [1246], 'cm2/mg': [1247], '(12Onoue': [1248], 'Yagi': [1250, 3784, 3854], 'Y.': [1251, 3785, 3855], 'Grossberg': [1252, 3786, 3856], 'A.L.': [1253, 3787, 3857], 'Pressman': [1254, 3788, 3858], 'D.': [1255, 3789, 3859], 'Immunochemistry.': [1256, 3790, 3860], '1965;': [1257, 3791, 3861], '2:': [1258, 3792, 3862], '401-415Crossref': [1259, 3793, 3863], 'coefficient.': [1266], 'measurements': [1269], 'radioactivity': [1272, 2156, 4259], 'assay,': [1273], 'solution': [1276, 1422, 2080, 2529, 2575, 3777, 3915, 3998, 4093, 4378], 'titrated': [1278], 'earlier(13Friguet': [1281], 'Chaffotte': [1283, 2677, 3006], 'A.F.': [1284, 2678, 3007], 'Methods.': [1291, 1379, 2685, 3014], '1985;': [1292, 2686, 3015], '77:': [1293, 2687, 3016], '305-319Crossref': [1294, 2688, 3017], '(1123)': [1297, 2691, 3020], 'Scholar).The': [1299], '1-102': [1301, 1646, 2945, 3564, 3845, 3896, 3930], '(14Fedorov': [1304], 'A.N.': [1305, 1374, 1614, 2708, 3537, 3961, 4183, 4298], 'Alakhov': [1310, 3542, 3966], 'Y.B.': [1311, 3543, 3967], '1992;': [1317, 3549, 3973], '228:': [1318, 3550, 3974], '351-358Crossref': [1319, 3551, 3975], '(58)': [1322, 3554, 3978], 'kindly': [1326], 'supplied': [1327], 'Dr.': [1329, 1677], 'Alexei': [1330], 'Fedorov.Epitope': [1331], 'Library': [1332], 'Construction,': [1333], 'Screening,': [1334], 'CharacterizationThe': [1336], 'library': [1338, 2847, 3454, 3701], 'constructed,': [1340], 'screened': [1341], 'clones': [1343, 1845, 1850, 2835, 2859], 'expressing': [1344, 2836, 3462], 'nucleotide': [1351], 'beginning': [1355], 'each': [1357, 1847, 2016, 2084, 2135, 2175, 2203, 2636, 4166, 4262], 'insert': [1358], 'well': [1360, 3110, 3926], 'length': [1363], 'et': [1370, 3003, 4179, 4294], 'al.(15Friguet': [1371], 'Fedorov': [1373, 1613, 2707, 4182, 4297], '1993;': [1380, 1623, 2717, 4192, 4307], '158:': [1381], '243-249Crossref': [1382], '(5)': [1385], 'Scholar).Cyanogen': [1387], 'Bromide': [1388], 'Cleavage': [1389], 'F1The': [1391], 'lyophilized': [1392], '(40': [1395], 'mg)': [1396], 'dissolved': [1398], '1': [1400, 2058, 2062, 2071, 2089, 2114, 2779, 2786, 3028, 3246, 3510, 3814, 4119], 'ml': [1401], '70%': [1403, 1930], 'formic': [1404], 'acid,': [1405], '100': [1406], 'mg': [1407], '(a': [1408, 1667], '20-fold': [1409, 2633], 'molar': [1410], 'excess': [1411], 'over': [1412], 'F1)': [1413], 'cyanogen': [1415, 2208, 2283, 2409, 2892], 'bromide': [1416, 2209, 2284, 2410, 2893], 'added,': [1418, 4140], 'incubated': [1424, 2550, 4117, 4141], 'overnight': [1425, 3341], 'dark': [1428], 'room': [1430, 2301], 'temperature.': [1431, 2302], 'mixture': [1433, 2056, 4076, 4115], 'then': [1435, 1935, 2577, 2889], 'diluted': [1436, 4078], '10-fold': [1437], 'water': [1439], 'lyophilized.Chemical': [1441], 'Synthesis': [1442, 2970], 'PeptidesPeptides': [1444], 'synthesized': [1446, 3756, 4062], 'Merrifield': [1449], '(16Merrifield': [1450], 'R.B.': [1451], 'Am.': [1453], 'Soc.': [1455], '1963;': [1456], '85:': [1457, 3099], '2149-2154Crossref': [1458], '(6610)': [1460], 'solid-phase': [1463], 'method': [1464, 2674, 2704], 'Applied': [1467, 1864, 2377, 2391], '430': [1468], 'synthesizer.': [1469], 'They': [1470], 'preparative': [1477], 'C18': [1478, 1498], 'reverse': [1479, 1499], 'phase': [1480, 1500], 'HPLC,': [1481], 'lyophilized,': [1482], 'stored': [1484], '4°C.': [1486], 'final': [1488, 2048], 'purity': [1489], '(>98%)': [1493], 'checked': [1495], 'analytical': [1497], 'HPLC.': [1501], 'Amino': [1502, 1511], 'composition': [1504], 'Beckman': [1509], '6300': [1510], 'Acid': [1512], 'Analyzer': [1513], 'after': [1514, 2144, 2628, 3999], '6': [1515], 'N': [1516], 'HCl': [1517], 'hydrolysis.': [1518], 'tyrosine': [1532, 1543], 'content,': [1533], 'coefficient': [1537], '1420': [1539], 'M−': [1540], 'cm−': [1541], '6.Site-directed': [1546], 'MutagenesisStop': [1547], 'codons': [1548, 1638, 1775, 1806, 3487, 3498, 3767], 'deletions': [1550], 'introduced': [1552, 1823, 3500], 'trpB': [1555, 1673], 'gene': [1556, 1687, 2846, 3484], 'oligonucleotide': [1558], 'site-directed': [1559, 3494], 'mutagenesis(17Kunkel': [1560], 'T.A.': [1561, 1726], 'Roberts': [1562, 1727], 'J.D.': [1563, 1728], 'Zakour': [1564, 1729], 'R.A.': [1565, 1730], 'Methods': [1566, 1731], '1987;': [1568, 1733, 2238], '154:': [1569, 1734], '367-382Crossref': [1570, 1735], '(4543)': [1573, 1738], '18Messing': [1576, 1741], 'Vieira': [1578, 1743], 'Gene': [1580, 1745], '(Amst.).': [1581, 1746], '1982;': [1582, 1747], '19:': [1583, 1748], '269-276Crossref': [1584, 1749], '(1717)': [1587, 1752], 'derivative': [1591, 1762, 1791], 'plasmid': [1594, 1661, 1668, 1914, 3220, 3327, 3532, 3561, 3574], 'pTZ19R,': [1595], 'carrying': [1596, 3251, 3320, 3325, 3335, 3384, 3420, 3481, 3582], 'coding': [1599, 2031], 'first': [1602, 2531, 2874], '102': [1603], 'acids': [1605, 2385], 'subunit(19Friguet': [1611], 'Serganov': [1615, 2709, 4184, 4299], 'A.A.': [1616, 2710, 4185, 4300], 'Navon': [1617, 2711, 4186, 4301], 'Anal.': [1621, 2236, 2336, 2715, 4190, 4305], '210:': [1624, 2718, 4193, 4308], '344-350Crossref': [1625, 2719, 4194, 4309], '(10)': [1628, 2722, 4197, 4312], 'introduction': [1635, 1650, 1810], 'stop': [1637, 1768, 1805, 3486, 3497, 3766], '15-21': [1642, 1786, 1830], 'deletion': [1643, 1654, 1787, 1822, 1831, 3254, 3568], 'peptide.': [1647, 2930], 'complete': [1657, 3333], 'derived': [1664], 'pαβTS21': [1666], 'trpA': [1671], 'genes,': [1674], 'constructed': [1675], 'Carlos': [1678], 'Zetina)': [1679], 'excising': [1681], 'B': [1686, 3213], 'ClaI': [1689, 1695], 'inserting': [1691], 'Bluescript': [1699, 1790], 'KS-.': [1700], 'single-stranded': [1702], 'DNA': [1703, 1758, 2850], 'plasmids': [1706, 1879, 3480], 'infection': [1710], 'strain': [1715], 'RZ1032,': [1716], 'cellular': [1720], 'host,': [1721], 'helper': [1723], 'phage': [1724], 'M13-K07(17Kunkel': [1725], 'single': [1756], 'strand': [1757], 'pTZ19R': [1761], 'introduce': [1766], 'codon': [1769], 'five': [1771, 2793], 'places': [1773, 3490], '(after': [1774], '39,': [1776, 3515, 3819], '50,': [1777, 3516, 3820], '60,': [1778, 3517, 3821], '69,': [1779, 3518, 3822], '79)': [1781], 'construct': [1784, 1793], 'deletion,': [1796, 3338], 'appropriate': [1798], 'primers.': [1799], 'construction': [1801], 'resulted': [1807, 1832, 2790], 'new': [1813, 1825], 'unique': [1814], 'AflII': [1815], 'restriction': [1816, 1854], 'site,': [1817, 1827], 'BamHI': [1826], 'loss': [1835], 'RsaI': [1838], 'site.': [1839], 'After': [1840], 'few': [1844], 'mutation,': [1848], 'correct': [1853], 'pattern': [1855], 'subjected': [1857, 2141, 2153, 3357, 3608, 4156], 'automated': [1859], 'Biosystem': [1865, 2378], 'sequencer,': [1866], 'T7': [1868, 1943], 'T3': [1870], 'primers': [1871], 'dye': [1874], 'termination': [1875], 'method.RNA': [1876], 'PreparationThe': [1877], '"mini-prep"': [1884], '"large': [1887], 'scale"(20Sambrook': [1888], 'Fritsch': [1890, 1946], 'E.F.': [1891, 1947], 'Maniatis': [1892, 1948], 'T.': [1893, 1949], 'Molecular': [1894, 1950], 'Cloning:': [1895, 1951], 'Laboratory': [1897, 1953], 'Manual.': [1898, 1954], '2nd': [1899, 1955], 'Ed.': [1900, 1956], 'Cold': [1901, 1905, 1957, 1961], 'Spring': [1902, 1906, 1958, 1962], 'Harbor': [1903, 1959], 'Laboratory,': [1904, 1960], 'Harbor,': [1907, 1963], 'NY1989Google': [1908, 1964], 'μg': [1911, 2059], 'linearized': [1918], 'EcoRI,': [1920], 'phenol': [1921], 'extracted,': [1922], 'ethanol-precipitated.': [1924], 'precipitate': [1926], 'washed': [1928], 'ethanol,': [1931], 'vacuum': [1932], 'dried,': [1933, 2151], 'transcription': [1940], 'promoter(20Sambrook': [1944], 'An': [1966], 'aliquot': [1967], 'newly': [1970], 'transcribed': [1971], 'RNA': [1972], 'loaded': [1974], '2%': [1977], 'agarose': [1978], 'verify': [1983], 'quality': [1985], 'RNA.In': [1988], 'Vitro': [1989], 'TranslationAll': [1990], 'translation': [1991, 2001, 2108, 2126, 2136, 2321], 'reactions': [1992, 2043], 'done': [1994], 'wheat': [1997, 2099, 3598, 3628, 4065], 'germ': [1998, 2100, 3599, 3629, 4066], 'lysate': [2002], '(Promega).': [2003], 'As': [2004], 'preliminary': [2006], 'step,': [2007], 'optimum': [2009], 'acetate': [2011], 'concentration': [2012, 2505, 2538], 'mRNA': [2017, 3762], '73': [2023], 'all': [2026, 3133, 3807], 'mRNAs,': [2027], 'except': [2028, 2087], '39': [2033, 3649], 'residue': [2034, 3469, 3579, 4399, 4438], '93': [2040], 'mM.': [2041], 'All': [2042, 2853], 'performed': [2045, 2212, 3667], 'volume': [2049], '25': [2051], 'μl.': [2052], 'typical': [2054], 'contained': [2057, 4260], 'RNA,': [2061], 'μl': [2063, 2069, 2076, 2090, 2097, 2104, 4072, 4081, 4088, 4102, 4125], 'Rnasin': [2065], '(54,000': [2066], 'units/ml),': [2067], '0.5': [2068], 'acetate,': [2074], '(1': [2081, 4091], 'methionine),': [2088], '[35S]methionine': [2092, 3754], '(Amersham': [2093], '1000': [2094], 'Ci/mmol),': [2095], '12.5': [2096], 'lysate,': [2101], '8': [2103], 'water.': [2106], 'allowed': [2110], 'proceed': [2112], 'h': [2115, 4120], '25°C.': [2117], 'check': [2121], 'efficiency': [2123], 'estimate': [2128, 2194, 2602], 'amount': [2130, 2197, 2524, 2605, 2621, 4161], 'produced': [2133, 2261, 2317, 2433, 2727, 3606], 'mixture,': [2137], '1-μl': [2138], 'aliquots': [2139], 'SDS-PAGE,': [2143, 4158], 'stained,': [2149], 'destained,': [2150], 'scanning': [2157, 4171], 'β-Imager': [2160, 2181, 4174, 4282], '(Biospace,': [2161], 'Paris,)': [2162], 'until': [2163], 'half': [2165], 'million': [2167], 'counts': [2168, 2173], 'recorded.': [2170, 4383], 'Integrating': [2171], 'means': [2178, 4413], 'software,': [2182, 4283], 'comparing': [2184], 'control': [2187, 2954], 'sticker': [2188], 'known': [2190], 'radioactivity,': [2191], 'provided': [2192, 2599], 'desired': [2200, 2357, 4111], 'peptide': [2201, 2909, 2932, 2944, 3033, 3048, 3051, 3064, 3871, 3950, 4059, 4381, 4443], 'preparation.Gel': [2204], 'ElectrophoresisElectrophoresis': [2205], 'Phast': [2216], 'System': [2217], '(Pharmacia)': [2218], 'homogeneous': [2220], 'SDS': [2221], '20': [2222], 'gels,': [2223], 'according': [2225, 2456, 3000], 'Schägger': [2227], 'von': [2229, 2233], 'Jagow': [2230, 2234], '(21Schägger': [2231], 'H.': [2232], 'G.': [2235], '166:': [2239], '368-379Crossref': [2240], '(10439)': [2243], 'molecular': [2247, 3395, 3431, 4211], 'weight': [2248, 3396, 4212], 'markers': [2249], '2350-46000': [2254], 'Da': [2255], 'kit': [2256], '(Amersham).For': [2257], 'translation,': [2265, 2437], 'logarithmic': [2267], '10-27%': [2268, 3362], 'acrylamide': [2269, 3363], 'gradient': [2270], 'used(22Laemmli': [2272], 'U.K.': [2273], 'Nature.': [2274], '227:': [2276], '680-685Crossref': [2277], '(206016)': [2280], 'Scholar).ElectrotransferThe': [2282], 'transferred': [2287, 2323], 'onto': [2288, 3729], 'Problott': [2289], 'membranes': [2290, 2326, 4016], '(Applied': [2291], 'Biosystems)': [2292], 'applying': [2294], '50': [2295, 4080], 'V': [2296], '30': [2298, 2552], 'min': [2299, 2553, 4144], '10': [2307, 4087], 'CHAPS': [2309], '11': [2312], '10%': [2314], 'methanol.The': [2315], 'nitro-cellulose': [2325], 'electrotransfer': [2328, 2400], '200': [2330, 4395], 'milliamperes': [2331], 'h(23Burnette': [2334], 'W.N.': [2335], '1981;': [2338], '112:': [2339], '195-203Crossref': [2340], '(5867)': [2343], 'Scholar).MicrosequencingAfter': [2345], 'staining': [2346], 'Amido': [2348], 'Schwarz,': [2349], 'region': [2351, 2758, 2825, 2987, 4393], 'membrane': [2354, 3728], 'band': [2358, 4263], 'cut': [2360], 'razor': [2363], 'blade,': [2364], 'three': [2366], 'cycles': [2367], 'determination': [2371], '470': [2379], 'gas-phase': [2380], 'automatic': [2381], 'sequencer.': [2382], 'Phenylthiohydantoin': [2383], 'identified': [2387, 2926], 'on-line': [2390], 'Biosystems': [2392], '120A': [2393], 'analyzer.Western': [2394], 'BlotsThe': [2395], 'membrane-bound': [2396], 'immunodetected': [2402], 'follows.': [2406, 4222], 'peptides,': [2411], 'rabbit': [2412, 2438], 'anti-mouse': [2413, 2439], 'IgG': [2414, 2440], 'coupled': [2416, 4132], 'alkaline': [2418], 'phosphatase': [2419], '(Biosys)': [2420], 'revealed': [2424, 2906], 'nitro': [2426], 'blue': [2427], 'tetrazolium/5-bromo-4-chloro-3-indolylphosphate': [2428], '(Sigma).': [2429], 'conjugated': [2442], 'horseraddish': [2444], 'peroxidase': [2445], 'ECL': [2452], 'reagents': [2453], 'Amersham': [2455], "manufacturer's": [2459], 'recommended': [2460], 'procedure.Competition': [2461], 'ELISAQuantification': [2462], 'truncated': [2467, 3236], 'crude': [2471, 2564, 2589], 'extracts': [2472], 'made': [2474, 2668, 4465], 'competition': [2477, 2584, 2613, 2672, 2702, 3781, 3850], 'ELISA': [2478, 2519, 2643, 2673], 'based': [2479], 'test': [2482, 3472], 'described(24Djavadi-Ohaniance': [2484], 'Butler': [2488, 2651], 'J.E.': [2489, 2652], 'Immunochemistry': [2491, 2654], 'Solid-phase': [2493, 2656], 'Immunoassay.': [2494, 2657], 'CRC': [2495, 2658], 'Press': [2496, 2659], 'Inc.,': [2497, 2660], 'Roca': [2498, 2661], 'Baton,': [2499, 2662], 'FL1991:': [2500, 2663], '201-206Google': [2501, 2664], 'Briefly,': [2503], 'range': [2506, 2545, 3144], 'last': [2514], 'indirect': [2518, 2580, 2642], 'proportional': [2521], 'free': [2526, 2571], 'determined.': [2532], 'Then,': [2533], 'mAb,': [2535], 'near': [2539], 'upper': [2541], 'limit': [2542], '(2.5': [2546], '10-10M)': [2548], 'various': [2555, 3489, 3765, 4104], 'dilutions': [2556, 4105], 'pure': [2560, 2597, 2695], 'extract': [2565, 2590], '(wild': [2566], 'mutant).': [2569], 'remaining': [2573], 'ELISA.': [2581], 'Comparing': [2582], 'curve': [2585, 2594], 'extract.': [2610, 4070], 'similar': [2612, 3887], 'assay': [2614], 'relative': [2620], 'fractions': [2626], 'filtration,': [2630], 'dilution': [2634], 'fraction.': [2637], 'procedure': [2639], 'previously(24Djavadi-Ohaniance': [2647], 'Scholar).Affinity': [2665], 'MeasurementsThey': [2666], '(13Friguet': [2675], 'when': [2694, 4013, 4042], 'antigen': [2696, 3623], 'available,': [2698], 'RIA': [2703, 3851], '(19Friguet': [2705], 'antigens': [2726], 'cell-free': [2731, 3600, 4002, 4067], 'synthesis.Circular': [2732], 'DichroismCircular': [2733], 'dichroism': [2734], 'spectra': [2735], 'recorded': [2737, 2803], 'CD6': [2740], 'spectrodichrograph': [2741], '(Jobin-Yvon,': [2742], 'Longjumeau,': [2743], 'France)': [2744], '0.1-': [2746], '0.2-mm': [2748], 'optical': [2749], 'path': [2750], 'cells.': [2751], 'temperature': [2753], '20°C.': [2755], 'wavelength': [2757, 2776], 'scanned': [2759], '193': [2762], '255': [2764], 'nm.': [2765, 2774], 'spectral': [2767], 'bandwidth': [2768], 'automatically': [2770], 'kept': [2771], 'increment': [2777], 'nm/step': [2780], 'accumulation': [2783], 'time': [2784], 's/step.': [2787], 'Each': [2788], 'spectrum': [2789, 2797, 2812, 2817, 4373, 4385, 4409], 'averaging': [2792], 'successive': [2794], 'scans.': [2795], 'solvent': [2800], 'alone': [2801], 'under': [2804, 3370], 'identical': [2805], 'conditions': [2806], 'substracted': [2808], 'sample': [2811], 'CD': [2816, 4372, 4456], 'peptide.RESULTSIn': [2820], 'identify': [2823], 'binds': [2831], '13': [2833], 'independent': [2834], 'recombinant': [2837, 3479], 'isolated': [2843, 4365, 4471], 'sequenced.': [2852], 'polypeptides': [2855], 'encoded': [2856, 3576], 'common': [2862], '1-85,': [2864], 'localized': [2866], 'within': [2871], '85': [2873], 'submitted': [2890], 'cleavage': [2894], 'analyzed': [2899], 'SDS-PAGE.': [2901], 'Staining': [2902], 'major': [2908], 'which,': [2910], 'migration': [2913], 'microsequencing': [2923], '(Pro-Ala-Leu),': [2924], '23-101': [2929], 'did': [2933, 3066, 3455, 3986], 'give': [2935], 'rise': [2936], 'any': [2938, 3459, 4422, 4460], 'distinctly': [2946], 'reacted': [2947, 3263, 3826], 'antibody.': [2955], 'These': [2956, 3152], 'results': [2957, 3024, 3633, 3799, 4044], '1-22': [2961, 2986, 3065], 'essential': [2964, 3304], 'building': [2966], 'up': [2967, 3458], 'epitope.Chemical': [2969], 'Immunoreactivity': [2972], 'Synthetic': [2974], 'PeptidesTo': [2975], 'delineate': [2978], 'epitope,': [2980], 'four': [2981], 'included': [2983], 'chemical': [2991], 'synthesis,': [2992], 'al.(13Friguet': [3004], 'Table': [3027, 3150, 4338], 'indicated': [3029, 3369, 3638, 3803, 3982], 'shortest': [3032], '1-9.': [3049], 'That': [3050], '2-22': [3052], 'showed': [3058], 'methionine': [3062, 3083], 'belong': [3068], 'epitope.': [3071], 'line': [3075, 4323, 4331], 'fact': [3078], 'that,': [3079, 3804], 'off': [3086], 'vivo(25Crawford': [3088], 'Decastel': [3090], 'Biophys.': [3095], 'Commun.': [3097], '1978;': [3098], '309-316Crossref': [3100], '(16)': [3103], 'reacts': [3109], 'mAb19.': [3112, 3244, 3380, 3829], 'minimal': [3114], 'thus': [3117, 3575, 4458], 'defined': [3118], 'therefore': [3119], 'corresponded': [3120], 'values': [3130, 3934], 'synthetic': [3135], '0.1-0.3': [3146], '109M−': [3148], '(see': [3149, 4337], '1).': [3151, 3898], 'only': [3155, 3940], 'smaller': [3157, 3942], 'than': [3158, 3687, 3943], '(0.6': [3160], '109M−)': [3162, 3891], 'suggesting': [3170], 'determinant': [3174, 3903], 'resides': [3180], 'sequence,': [3184], 'rest': [3188], 'might': [3193, 3742], 'contribute': [3195], 'recognition.Tabled': [3199], '1Preparation': [3200], 'Characterization': [3202], 'Mutants': [3204], 'Deletions': [3206], 'Stop': [3208], 'Codons': [3209], 'tryp': [3212], 'GeneTo': [3214], 'ascertain': [3215], 'latter': [3217, 3573], 'conclusion': [3218, 4462], 'encoding': [3221, 3328, 3533, 3562], 'prepared,': [3229], 'immunoreactivity': [3232, 3307], 'corresponding': [3235, 3588], 'investigated': [3239, 3779, 3810], 'blotting': [3242], 'Fig.': [3245, 3878], 'shows': [3247], 'reactivity': [3258, 4008], 'toward': [3259, 3312], 'mAb164,': [3266], '273-283': [3273, 3412], 'chain(26Larvor': [3277], 'M.-P.': [3278], 'Baleux': [3283], 'F.': [3284], '28:': [3290], '523-531Crossref': [3291], '(13)': [3294], 'confirmed': [3298], 'mAb19.Figure': [3313], '1:Western': [3314], 'blots': [3315, 3663, 4041], 'deletion.': [3323], 'Bacteria': [3324], 'grown': [3340], 'rich': [3343], 'medium': [3344], 'supplemented': [3345, 4085], 'ampicillin,': [3347], 'washed,': [3348], 'sonicated,': [3349], 'centrifuged.': [3351], 'supernatants': [3353], '(15': [3354], 'μl)': [3355], 'SDS-PAGE': [3359, 3610], 'gel,': [3364], 'electrotransferred,': [3365], 'stained': [3367], '"Materials': [3371], 'Methods."': [3373], 'Left': [3374], '(A):': [3376], 'blot': [3378, 3402], 'Slot': [3381, 3417, 4209, 4223], '1,': [3382, 4210], 'deletion;': [3387, 3423], 'slot': [3388, 3393, 3424, 3429, 4167, 4226, 4231, 4236, 4241, 4246, 4251], '2,': [3389, 4224], 'β;': [3392, 3428], '3,': [3394, 4227], 'markers.': [3397], 'Right': [3398], '(B):': [3400], 'recognizes': [3409], '4,': [3418, 4232], '5,': [3425, 4237], '6,': [3430, 4242], 'mass': [3432], '(indicated': [3433], 'kDa)': [3435], 'markers.View': [3436], 'Large': [3437, 4340], 'Image': [3438, 4341], 'Figure': [3439, 4342], 'ViewerDownload': [3440, 4343], 'Hi-res': [3441, 4344], 'image': [3442, 4345], 'Download': [3443, 4346], '(PPT)This': [3444], 'raised': [3445], 'question': [3447], 'why': [3449], 'screening': [3450, 3708, 3714], 'pick': [3457], 'clone': [3461], 'would': [3466, 3746], 'end': [3467], 'before': [3468], '85.': [3470], 'To': [3471], 'possibility': [3474], 'cloning': [3477, 3689], 'artifact,': [3478, 3690], 'trypB': [3483], 'mutagenesis.': [3495], 'positions': [3502, 3514, 3818], 'such': [3503], 'starting': [3507], 'position': [3509, 3813, 4266], 'ending': [3512, 3816], '79': [3520, 3651], 'should': [3521, 4035], 'produced.': [3523], 'Two': [3524], 'positive': [3525], 'controls': [3526], 'used.': [3528], 'One': [3529], '1-102(14Fedorov': [3536], 'second': [3558], '15-21.': [3571], '95': [3578], '(1-14.22-102)': [3581], 'sequence.': [3586], 'mRNAs': [3589], 'templates': [3595], 'synthesis': [3602, 4069, 4075], 'system.': [3603], 'blotting.': [3613], 'Although': [3614], 'considerably': [3615], 'blurred': [3616], 'endogenous': [3622], 'likely': [3624], 'synthase,': [3631], '(not': [3635], 'shown)': [3636], 'clearly': [3637, 3682], 'extending': [3641], 'anywhere': [3646], 'here.': [3668], 'On': [3669], 'contrary,': [3671], 'longer': [3672], 'intact': [3676], 'seen.': [3684], 'rather': [3686], 'failure': [3692], 'shorter': [3695], 'due': [3705, 3717], 'artifact.To': [3709], 'find': [3710], 'whether': [3712], 'artifact': [3715, 4023], 'abnormal': [3720, 3990, 4453], 'interaction': [3721, 3772], 'short': [3724, 3740, 3994, 4011], 'they': [3731], 'blotted,': [3733], 'particular': [3737], 'adopt': [3743, 3988], 'render': [3747], 'unreactive,': [3750], 'labeled': [3752], 'above,': [3760], 'templates,': [3769], '(10-8M)': [3775], 'RIA(12Onoue': [3782], 'qualitative': [3798], 'experiment': [3802], 'solution,': [3806, 3847], '(starting': [3811], '79,': [3824], 'respectively)': [3825], 'quantitatively': [3832], 'verified': [3833], 'measuring': [3835], '1-60': [3843, 3872, 3906, 3923, 4060, 4269], 'method(12Onoue': [3852], 'data': [3875, 4286], '0.75': [3881], '109': [3883], '(0.7': [3889], '(Table': [3897], 'blotting,': [3913], 'fragment.': [3931], 'Moreover,': [3932, 4432], 'reported': [3945], 'above': [3946], 'observed': [3956], 'β2(14Fedorov': [3960], 'synthesis.': [4003], 'apparently': [4006], 'poor': [4007], 'immobilized': [4014], 'primarily': [4020], 'immunodetection': [4026], 'showing': [4032], 'care': [4034], 'taken': [4037], 'interpreting': [4039], 'negative': [4043], 'obtained.Figure': [4046], '2:Determination': [4047], '1-60.': [4056, 4208], '35S-labeled': [4058], 'caseine': [4090], 'mg/ml': [4092, 4377], 'filtered': [4094], '0.45-μm': [4097], 'Millipore': [4098], 'filter)': [4099], '1-3': [4101], 'obtain': [4109], 'concentration.': [4113, 4444], '4°C,': [4122], '40': [4124], 'suspension': [4128], 'Sepharose': [4130], 'beads': [4131], 'saturated': [4136], 'casein': [4138], 'gentle': [4146], 'shaking,': [4147], 'centrifuged,': [4148], 'washed.': [4150], 'immunoadsorbed': [4152], 'dissolved,': [4155], 'radioactive': [4163], 'al.(19Friguet': [4180, 4295], 'A,': [4200], 'bidimensional': [4201], 'scan': [4202], 'markers;': [4213], 'mixtures': [4219], '0;': [4225], '2.3': [4228, 4238, 4248], '10-10M;': [4230, 4235], '6.8': [4233, 4243, 4253], '10-9M;': [4240, 4245], '7,': [4247], '10-8M;': [4250], '8,': [4252], '10-8M.': [4255], 'B,': [4256], 'total': [4258], 'diagram': [4272], 'plotted': [4289], 'points': [4317], 'fitted': [4319], 'straight': [4322], 'linear': [4325], 'regression.': [4326], 'slope': [4328], 'provides': [4332], 'equilibrium': [4334], 'dissociation': [4335], '1).View': [4339], '(PPT)Far': [4347], 'UV': [4348, 4371, 4455], 'Circular': [4349], 'Dichroism': [4350], 'Antigenic': [4354], 'PeptideIn': [4355], 'nonapeptide,': [4368], 'far': [4370, 4454], 'quite': [4388], 'atypical,': [4389], 'mainly': [4390], 'below': [4394], 'ellipticity': [4400, 4439], 'particularly': [4402], 'low': [4403], '(about': [4404], '−12,000': [4405], 'deg•cm-2•dmol−).': [4406], 'Such': [4407], 'generated': [4415], 'random': [4418], 'classical': [4425], 'secondary': [4426], 'backbone.': [4431], 'amplitude': [4434], 'mean': [4437], 'aggregates': [4448], 'responsible': [4450], 'spectrum,': [4457], 'precluding': [4459], 'reliable': [4461], 'concerning': [4466], 'nonapeptide': [4472], 'lower': [4476], 'measurements.Expressi': [4481]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1972067409', 'counts_by_year': [{'year': 2020, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 1}], 'updated_date': '2024-12-12T13:41:25.806111', 'created_date': '2016-06-24'}