Title: Use of immunoglobulin light chain analysis to detect bone marrow involvement in B-cell neoplasms
Abstract: Abstract Double-blind analysis of bone marrow specimens from 15 patients with B-cell neoplasms and 10 controls was performed to determine whether immunoglobulin (Ig) light chain analysis (LCA) of bone marrow lymphoid cells is a more sensitive indicator of bone marrow involvement than light microscopic (LM) examination. Bone marrow aspirates were evaluated by a two-color immunofluorescence (IF) microscopic technique to determine the ratio of κ-bearing lymphocytes to λ-bearing lymphocytes ( κ λ ratio). Both bone marrow aspirate and biopsy specimens were reviewed by LM. Abnormal κ λ ratios implied bone marrow involvement in 9 10 cases of non-Hodgkin's lymphoma (NHL), 4 4 cases of multiple myeloma (MM), and 1 1 case of Waldenstrom's macroglobulinemia (WM). All MM and WM cases had evidence for involvement by LM. Two NHL patients with abnormal κ λ ratios had normal bone marrow morphology. LCA implied bone marrow involvement in 4 5 NHL patients with equivocal LM evaluations and in 3 3 cases with clear morphologic involvement. These data suggest that combined LCA and LM evaluation represent a more objective and inclusive method to detect bone marrow involvement in B-cell neoplasms than LM alone.
Publication Year: 1982
Publication Date: 1982-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 5
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