Title: Two Rubisco activase isoforms may play different roles in photosynthetic heat acclimation in the rice plant
Abstract: Physiologia PlantarumVolume 139, Issue 1 p. 55-67 Two Rubisco activase isoforms may play different roles in photosynthetic heat acclimation in the rice plant Dun Wang, Dun Wang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorXiao-Fei Li, Xiao-Fei Li State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorZheng-Jian Zhou, Zheng-Jian Zhou State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorXu-Ping Feng, Xu-Ping Feng State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorWan-Jun Yang, Wan-Jun Yang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorDe-An Jiang, Corresponding Author De-An Jiang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China* e-mail: [email protected] for more papers by this author Dun Wang, Dun Wang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorXiao-Fei Li, Xiao-Fei Li State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorZheng-Jian Zhou, Zheng-Jian Zhou State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorXu-Ping Feng, Xu-Ping Feng State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorWan-Jun Yang, Wan-Jun Yang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, ChinaSearch for more papers by this authorDe-An Jiang, Corresponding Author De-An Jiang State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China* e-mail: [email protected] for more papers by this author First published: 08 April 2010 https://doi.org/10.1111/j.1399-3054.2009.01344.xCitations: 55Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Studies on some plant species have shown that increasing the growth temperature gradually or pretreating with high temperature can lead to obvious photosynthetic acclimation to high temperature. To test whether this acclimation arises from heat adaptation of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) activation mediated by Rubisco activase (RCA), gene expression of RCA large isoform (RCAL) and RCA small isoform (RCAS) in rice was determined using a 4-day heat stress treatment [40/30°C (day/night)] followed by a 3-day recovery under control conditions [30/22°C (day/night)]. The heat stress significantly induced the expression of RCAL as determined by both mRNA and protein levels. Correlative analysis indicated that RCAS protein content was extremely significantly related to Rubisco initial activity and net photosynthetic rate (Pn) under both heat stress and normal conditions. Immunoblot analysis of the Rubisco–RCA complex revealed that the ratio of RCAL to Rubisco increased markedly in heat-acclimated rice leaves. Furthermore, transgenic rice plants expressing enhanced amounts of RCAL exhibited higher thermotolerance in Pn and Rubisco initial activity and grew better at high temperature than wild-type (WT) plants and transgenic rice plants expressing enhanced amounts of RCAS. Under normal conditions, the transgenic rice plants expressing enhanced amounts of RCAS showed higher Pn and produced more biomass than transgenic rice plants expressing enhanced amounts of RCAL and wild-type plants. Together, these suggest that the heat-induced RCAL may play an important role in photosynthetic acclimation to moderate heat stress in vivo, while RCAS plays a major role in maintaining Rubisco initial activity under normal conditions. Citing Literature Supporting Information Supporting Information Additional Supporting Information may be found in the online version of this article: Appendix S1. Expressed protein fragment of RCA and monoclonal antibodies to Rubisco subunits and RCA isoforms. Appendix S2. Stoichiometry of RCA protein in the Rubisco–RCA complex. Please note: Wiley-Blackwell are not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Filename Description PPL_1344_sm_AppendixS1-2.pdf438.4 KB Supporting info item Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume139, Issue1May 2010Pages 55-67 RelatedInformation
Publication Year: 2010
Publication Date: 2010-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 85
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