Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1970257327', 'doi': 'https://doi.org/10.1002/mrd.20221', 'title': 'Effect of leukemia inhibitory factor (LIF) on in vitro produced bovine embryos and their outgrowth colonies', 'display_name': 'Effect of leukemia inhibitory factor (LIF) on in vitro produced bovine embryos and their outgrowth colonies', 'publication_year': 2005, 'publication_date': '2005-01-01', 'ids': {'openalex': 'https://openalex.org/W1970257327', 'doi': 'https://doi.org/10.1002/mrd.20221', 'mag': '1970257327', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/15685635'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/mrd.20221', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S125242520', 'display_name': 'Molecular Reproduction and Development', 'issn_l': '1040-452X', 'issn': ['1040-452X', '1098-2795'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5041383696', 'display_name': 'Morten Vejlsted', 'orcid': None}, 'institutions': [], 'countries': ['DK'], 'is_corresponding': False, 'raw_author_name': 'Morten Vejlsted', 'raw_affiliation_strings': ['Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark'], 'affiliations': [{'raw_affiliation_string': 'Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5023392514', 'display_name': 'B. Avery', 'orcid': None}, 'institutions': [], 'countries': ['DK'], 'is_corresponding': False, 'raw_author_name': 'Birthe Avery', 'raw_affiliation_strings': ['Department of Large Animal Sciences, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark'], 'affiliations': [{'raw_affiliation_string': 'Department of Large Animal Sciences, Reproduction, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark', 'institution_ids': []}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5031784413', 'display_name': 'Jakob O. Gjørret', 'orcid': None}, 'institutions': [], 'countries': ['DK'], 'is_corresponding': False, 'raw_author_name': 'Jakob Oemar Gjorret', 'raw_affiliation_strings': ['Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark'], 'affiliations': [{'raw_affiliation_string': 'Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark', 'institution_ids': []}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5074733593', 'display_name': 'P. Maddox‐Hyttel', 'orcid': None}, 'institutions': [], 'countries': ['DK'], 'is_corresponding': False, 'raw_author_name': 'Poul Maddox-Hyttel', 'raw_affiliation_strings': ['Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark'], 'affiliations': [{'raw_affiliation_string': 'Department of Animal and Veterinary Basic Sciences, Anatomy and Cell Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark', 'institution_ids': []}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 0, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 4020, 'currency': 'USD', 'value_usd': 4020, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 2.287, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 43, 'citation_normalized_percentile': {'value': 0.930962, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 92, 'max': 93}, 'biblio': {'volume': '70', 'issue': '4', 'first_page': '445', 'last_page': '454'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10364', 'display_name': 'Fertility Preservation in Cancer Patients', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/2739', 'display_name': 'Public Health, Environmental and Occupational Health'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10364', 'display_name': 'Fertility Preservation in Cancer Patients', 'score': 0.999, 'subfield': {'id': 'https://openalex.org/subfields/2739', 'display_name': 'Public Health, Environmental and Occupational Health'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10505', 'display_name': 'Induction and Differentiation of Pluripotent Stem Cells', 'score': 0.998, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12929', 'display_name': 'Transgenic Animal Research', 'score': 0.9895, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/inner-cell-mass', 'display_name': 'Inner cell mass', 'score': 0.8869573}, {'id': 'https://openalex.org/keywords/embryo-development', 'display_name': 'Embryo Development', 'score': 0.502671}, {'id': 'https://openalex.org/keywords/subculture', 'display_name': 'Subculture (biology)', 'score': 0.42759907}], 'concepts': [{'id': 'https://openalex.org/C27284151', 'wikidata': 'https://www.wikidata.org/wiki/Q6534502', 'display_name': 'Leukemia inhibitory factor', 'level': 4, 'score': 0.92546326}, {'id': 'https://openalex.org/C159399396', 'wikidata': 'https://www.wikidata.org/wiki/Q1335221', 'display_name': 'Inner cell mass', 'level': 5, 'score': 0.8869573}, {'id': 'https://openalex.org/C2778177303', 'wikidata': 'https://www.wikidata.org/wiki/Q227717', 'display_name': 'Blastocyst', 'level': 4, 'score': 0.8612612}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.75883764}, {'id': 'https://openalex.org/C196843134', 'wikidata': 'https://www.wikidata.org/wiki/Q33196', 'display_name': 'Embryo', 'level': 2, 'score': 0.60920066}, {'id': 'https://openalex.org/C16685009', 'wikidata': 'https://www.wikidata.org/wiki/Q335057', 'display_name': 'Andrology', 'level': 1, 'score': 0.59018886}, {'id': 'https://openalex.org/C202751555', 'wikidata': 'https://www.wikidata.org/wiki/Q221681', 'display_name': 'In vitro', 'level': 2, 'score': 0.50940615}, {'id': 'https://openalex.org/C145103041', 'wikidata': 'https://www.wikidata.org/wiki/Q1151519', 'display_name': 'Embryonic stem cell', 'level': 3, 'score': 0.4731627}, {'id': 'https://openalex.org/C2779814568', 'wikidata': 'https://www.wikidata.org/wiki/Q58345', 'display_name': 'Laminin', 'level': 3, 'score': 0.45083842}, {'id': 'https://openalex.org/C87863119', 'wikidata': 'https://www.wikidata.org/wiki/Q842756', 'display_name': 'Subculture (biology)', 'level': 2, 'score': 0.42759907}, {'id': 'https://openalex.org/C81885089', 'wikidata': 'https://www.wikidata.org/wiki/Q189082', 'display_name': 'Cell culture', 'level': 2, 'score': 0.41067803}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.39798093}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.32639974}, {'id': 'https://openalex.org/C1491633281', 'wikidata': 'https://www.wikidata.org/wiki/Q7868', 'display_name': 'Cell', 'level': 2, 'score': 0.31262696}, {'id': 'https://openalex.org/C87073359', 'wikidata': 'https://www.wikidata.org/wiki/Q33196', 'display_name': 'Embryogenesis', 'level': 3, 'score': 0.30704546}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.30656356}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.12887633}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.10941148}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.09638825}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D004622', 'descriptor_name': 'Embryo, Mammalian', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': True}, {'descriptor_ui': 'D005307', 'descriptor_name': 'Fertilization in Vitro', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D015850', 'descriptor_name': 'Interleukin-6', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002417', 'descriptor_name': 'Cattle', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004622', 'descriptor_name': 'Embryo, Mammalian', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D066298', 'descriptor_name': 'In Vitro Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015850', 'descriptor_name': 'Interleukin-6', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D053605', 'descriptor_name': 'Leukemia Inhibitory Factor', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/mrd.20221', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S125242520', 'display_name': 'Molecular Reproduction and Development', 'issn_l': '1040-452X', 'issn': ['1040-452X', '1098-2795'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/15685635', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 60, 'referenced_works': ['https://openalex.org/W1911593833', 'https://openalex.org/W1964741712', 'https://openalex.org/W1968577363', 'https://openalex.org/W1971843304', 'https://openalex.org/W1974421415', 'https://openalex.org/W1979981202', 'https://openalex.org/W1980417131', 'https://openalex.org/W1983755641', 'https://openalex.org/W1984934521', 'https://openalex.org/W1988600076', 'https://openalex.org/W1989194294', 'https://openalex.org/W1992160731', 'https://openalex.org/W1995559307', 'https://openalex.org/W1995659623', 'https://openalex.org/W1999637863', 'https://openalex.org/W2004575443', 'https://openalex.org/W2006499356', 'https://openalex.org/W2010422006', 'https://openalex.org/W2012230887', 'https://openalex.org/W2014165073', 'https://openalex.org/W2018855387', 'https://openalex.org/W2022797781', 'https://openalex.org/W2023116564', 'https://openalex.org/W2027781586', 'https://openalex.org/W2028550077', 'https://openalex.org/W2031280968', 'https://openalex.org/W2039850708', 'https://openalex.org/W2041995298', 'https://openalex.org/W2044155412', 'https://openalex.org/W2045899482', 'https://openalex.org/W2046617994', 'https://openalex.org/W2047367562', 'https://openalex.org/W2052332932', 'https://openalex.org/W2052672994', 'https://openalex.org/W2057159461', 'https://openalex.org/W2057454317', 'https://openalex.org/W2060937867', 'https://openalex.org/W2063701555', 'https://openalex.org/W2064225457', 'https://openalex.org/W2065786458', 'https://openalex.org/W2072526865', 'https://openalex.org/W2086943348', 'https://openalex.org/W2090134173', 'https://openalex.org/W2090615247', 'https://openalex.org/W2093315651', 'https://openalex.org/W2106778371', 'https://openalex.org/W2111905668', 'https://openalex.org/W2126851387', 'https://openalex.org/W2132545788', 'https://openalex.org/W2132555347', 'https://openalex.org/W2133501984', 'https://openalex.org/W2138442302', 'https://openalex.org/W2139004073', 'https://openalex.org/W2143297890', 'https://openalex.org/W2144142672', 'https://openalex.org/W2144641040', 'https://openalex.org/W2150163104', 'https://openalex.org/W2332649045', 'https://openalex.org/W2408877013', 'https://openalex.org/W27140539'], 'related_works': ['https://openalex.org/W2373479018', 'https://openalex.org/W2353791190', 'https://openalex.org/W2223970316', 'https://openalex.org/W2123965772', 'https://openalex.org/W2097300309', 'https://openalex.org/W2039065568', 'https://openalex.org/W2028550077', 'https://openalex.org/W2003026164', 'https://openalex.org/W173475486', 'https://openalex.org/W1693819034'], 'abstract_inverted_index': {'In': [0, 99, 278], 'vitro': [1, 10, 287], 'produced': [2], '(IVP)': [3], 'bovine': [4], 'embryos': [5, 55, 95, 100], 'were': [6, 38, 109], 'subjected': [7], 'to': [8, 24, 31, 202, 209, 292, 302], 'in': [9, 46, 94, 128, 140, 146, 174, 180, 260, 286, 296], 'culture': [11, 26, 263, 294], 'with': [12, 49, 66, 102], 'or': [13, 50, 275], 'without': [14, 51, 97, 158], '1000': [15], 'U/ml': [16], 'human': [17], 'recombinant': [18], 'leukemia': [19], 'inhibitory': [20], 'factor': [21], '(LIF)': [22], 'added': [23, 291], 'the': [25, 57, 62, 132, 143, 147, 155, 177, 181, 203, 210, 214, 225, 261, 272, 293, 297, 312], 'medium': [27, 48, 264, 295], 'from': [28, 299, 316], 'Days': [29, 300], '5': [30, 301], '8': [32, 78, 218, 303], 'post': [33], 'insemination': [34], '(p.i.).': [35], 'Resulting': [36], 'blastocysts': [37, 65, 134, 144, 156, 178], 'subsequently': [39, 314], 'plated': [40, 165], 'intact': [41], 'on': [42, 166, 271, 285, 311], 'mouse': [43], 'feeder': [44, 204], 'cells': [45, 197, 234], 'a': [47, 112, 189, 228], 'LIF.': [52, 98, 150, 184], 'Significantly': [53], 'more': [54], 'reached': [56], 'hatched': [58], 'blastocyst': [59], 'stage,': [60], 'and': [61, 84, 106, 111, 118, 241, 253], 'number': [63], 'of': [64, 115, 131, 142, 149, 154, 176, 183, 194, 213, 219, 224, 231, 250, 258], 'excellent': [67], 'morphology': [68], 'was': [69, 74, 91, 126, 138, 171, 198, 206], 'significantly': [70, 92], 'higher,': [71], 'when': [72, 164, 200, 290], 'LIF': [73, 159, 259, 280], 'omitted.': [75], 'At': [76, 216], 'Day': [77, 217], 'p.i.,': [79, 304], 'total': [80], 'cell': [81, 86, 89], 'count': [82, 90], '(TCC)': [83], 'inner': [85], 'mass': [87], '(ICM)': [88], 'higher': [93], 'cultured': [96, 101, 145, 157, 179], 'LIF,': [103], 'cytoplasmic': [104], 'vesicles': [105], 'lipid': [107], 'droplets': [108], 'abundant': [110], 'decreased': [113], 'expression': [114], 'both': [116, 251], 'Oct4': [117, 247], 'laminin': [119], 'could': [120], 'be': [121], 'observed.': [122], 'Initial': [123], 'hypoblast': [124], 'formation': [125], 'revealed': [127, 248], 'almost': [129, 152], '1/3': [130], 'LIF-cultured': [133], 'whereas': [135, 168, 305], 'this': [136, 169], 'feature': [137], 'evident': [139], '2/3': [141], 'absence': [148], 'Overall,': [151], '60%': [153], 'formed': [160, 315], 'outgrowth': [161, 220, 262], 'colonies': [162, 226], '(OCs)': [163], 'feeders,': [167], 'phenomenon': [170], 'only': [172], 'observed': [173], '30%': [175], 'presence': [182, 257], 'A': [185], 'tendency': [186], 'for': [187, 238, 246], 'retaining': [188], 'tightly': [190], 'packed': [191], 'central': [192, 229], 'growth': [193], 'putative': [195, 232], 'ICM-derived': [196, 233, 254], 'observed,': [199], 'attachment': [201], 'layer': [205], 'initiated': [207], 'close': [208], 'embryonic': [211, 288], 'pole': [212], 'blastocyst.': [215], 'culture,': [221], 'approximately': [222], '20%': [223], 'contained': [227], 'core': [230], 'appearing': [235], 'large': [236], 'enough': [237], 'mechanical': [239], 'isolation': [240], 'further': [242], 'subculture.': [243], 'Immunohistochemical': [244], 'labeling': [245], 'staining': [249], 'trophectodermal': [252], 'cells.': [255], 'The': [256], 'did': [265], 'not': [266], 'have': [267], 'any': [268], 'apparent': [269, 309], 'effect': [270, 284, 310], 'plating': [273], 'efficiency': [274], 'colony': [276], 'type.': [277], 'conclusion,': [279], 'had': [281, 307], 'an': [282], 'adverse': [283], 'development': [289], 'period': [298], 'it': [306], 'no': [308], 'OCs': [313], 'such': [317], 'embryos.': [318], 'Mol.': [319], 'Reprod.': [320], 'Dev.': [321], '70:': [322], '445–454,': [323], '2005.': [324], '©': [325], '2005': [326], 'Wiley-Liss,': [327], 'Inc.': [328]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1970257327', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 4}, {'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 2}, {'year': 2014, 'cited_by_count': 5}, {'year': 2013, 'cited_by_count': 4}], 'updated_date': '2024-09-25T14:08:33.270146', 'created_date': '2016-06-24'}