Title: Agrobacterium tumefaciens-mediated genetic transformation of the entomopathogenic fungus Beauveria bassiana
Abstract: Agrobacterium tumefaciens-mediated transformation (agro-transformation) was successfully applied to the entomogenous fungus Beauveria bassiana. Conidia of B. bassiana were transformed to hygromycin B resistance using the hph gene of Escherichia coli as the selective trait, under the control of a heterologous fungal promoter and the Aspergillus nidulans trpC terminator. The efficiency of transformation was up to 28 and 96 transformants per 104 and 105 target conidia, respectively, using three distinct vectors. High mitotic stability of the transformants (80–100%) was demonstrated after five successive transfers on a nonselective medium. Abortive transformants were observed for all the hphr vectors used. Putative transformants were analysed for the presence of the hph gene by PCR and Southern analysis. The latter analysis revealed the integration of two or more copies of the hph gene in the genome. The agro-transformation method was found to be effective for the isolation of B. bassiana hygromycin resistant transformants and may represent a useful tool for insertional mutagenesis studies in this fungus.
Publication Year: 2004
Publication Date: 2004-05-13
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 84
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