Title: Efficient Trans-Splicing of a Yeast Mitochondrial RNA Group II Intron Implicates a Strong 5′ Exon-Intron Interaction
Abstract: The reaction mechanism for self-splicing introns requires the existence of a 5′ exon binding site on the intron. Experimental evidence is now presented consistent with the existence of such a binding site by demonstrating efficient and accurate trans-self-splicing of a yeast mitochondrial group II intron. Partial and complete trans-splicing reactions take place in the absence of branch formation, part of the usual pathway of nuclear splicing and group II self-splicing. In addition to indicating the existence of a 5′ exon binding site on the intron, the results have mechanistic implications for group II self-splicing and perhaps for nuclear splicing as well.
Publication Year: 1986
Publication Date: 1986-11-28
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 76
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