Publication Information

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2809, 2816, 2852, 2862, 2883, 2906, 2911, 2959, 2974, 3077, 3092, 3124, 3156, 3184, 3317, 3365, 3388, 3407, 3503, 3530, 3718, 3725, 3944, 4062, 4085, 4099, 4112, 4128, 4277, 4324, 4349, 4412, 4444, 4508], 'sensory': [8, 242, 3547], 'stimuli,': [9, 243], 'act': [10, 244], 'as': [11, 245, 626, 716, 771, 774, 796, 958, 975, 1376, 1516, 1608, 2045, 2147, 2348, 2515, 2822, 3217, 3322, 3324, 3578, 4159, 4226], 'molecular': [12, 246, 1622, 2308], 'switches': [13, 247], 'to': [14, 17, 68, 210, 248, 251, 302, 444, 678, 968, 1325, 1389, 1428, 1541, 1575, 1612, 1629, 1707, 1736, 1886, 1888, 1930, 1941, 2033, 2228, 2413, 2485, 2554, 2689, 2805, 2917, 3045, 3227, 3250, 3301, 3346, 3361, 3403, 3409, 3556, 3643, 3676, 3688, 3736, 3791, 3801, 3918, 3983, 4018, 4063, 4119, 4147, 4223, 4286, 4334, 4371, 4499, 4509], 'relay': [15, 249], 'activation': [16, 250, 668, 1511], 'heterotrimeric': [18, 252, 691], 'proteins.': [20, 254, 693, 3851, 3906], 'Recent': [21, 255], 'studies': [22, 256, 759, 903, 1286, 1310, 1395, 1729, 1810, 1857, 2124, 4312], 'suggest': [23, 257, 4433], 'that': [24, 33, 49, 92, 107, 181, 220, 258, 267, 283, 326, 341, 415, 454, 579, 637, 906, 970, 1033, 1267, 1292, 1368, 1390, 1472, 1506, 1713, 1850, 1870, 2074, 2103, 2116, 2129, 2193, 2204, 3005, 3224, 3239, 3427, 3437, 3441, 3602, 3619, 3650, 3696, 3756, 3776, 3993, 3996, 4006, 4052, 4065, 4079, 4136, 4176, 4237, 4269, 4434, 4445], 'GPCRs': [25, 48, 259, 282, 617, 642, 769, 892, 913, 1432, 3803, 4481], 'form': [26, 223, 260, 457, 790, 914, 1873, 3251, 4437, 4467], 'dimeric': [27, 261, 3542, 3572], 'or': [28, 137, 190, 229, 262, 371, 424, 463, 773, 852, 1061, 2183, 2807, 3153, 3246, 3284, 3969, 4420], 'oligomeric': [29, 263], 'structures,': [30, 264, 794], 'a': [31, 65, 96, 130, 177, 265, 299, 330, 364, 411, 619, 672, 764, 907, 1271, 1351, 1381, 1448, 1462, 1502, 1545, 1609, 1709, 1882, 2104, 2178, 2184, 2200, 2225, 2419, 2431, 2668, 2690, 2789, 2927, 2980, 3207, 3218, 3222, 3235, 3243, 3252, 3281, 3298, 3342, 3419, 3463, 3475, 3484, 3498, 3549, 3575, 3680, 3702, 3860, 4000, 4058, 4227, 4279, 4283, 4360, 4372], 'phenomenon': [32, 266], 'has': [34, 268, 1036, 1633, 1665, 1938, 2029, 3289, 3798, 3915, 4141, 4234, 4418, 4461], 'long': [35, 269], 'been': [36, 270, 1666, 1705, 1939, 2031, 3758, 3799, 3916, 4235], 'established': [37, 271], 'for': [38, 57, 198, 272, 291, 432, 684, 1209, 1616, 1639, 2199, 2444, 2468, 2613, 2629, 2682, 2732, 2769, 2782, 2920, 2950, 2956, 2963, 3048, 3089, 3129, 3136, 3383, 3671, 3845, 4339, 4409], 'growth': [39, 273], 'factor': [40, 274, 516, 565, 1458], 'receptors.': [41, 275, 1935], 'The': [42, 276, 1365, 2217, 2268, 2407, 2481, 2594, 2616, 2632, 2673, 2685, 2696, 2724, 2738, 2762, 2913, 2930, 2942, 2966, 3054, 3082, 3143, 3394, 3457, 3489, 3912, 3979], 'elucidation': [43, 277], 'of': [44, 47, 54, 61, 77, 114, 145, 160, 166, 201, 278, 281, 288, 295, 311, 348, 379, 394, 400, 435, 576, 621, 633, 676, 682, 690, 700, 760, 780, 855, 887, 891, 897, 901, 911, 951, 955, 1019, 1069, 1071, 1074, 1078, 1276, 1279, 1283, 1307, 1311, 1321, 1359, 1396, 1398, 1425, 1464, 1468, 1539, 1553, 1565, 1573, 1624, 1644, 1711, 1730, 1744, 1817, 1831, 1892, 1933, 1946, 1948, 2036, 2042, 2077, 2090, 2136, 2154, 2165, 2172, 2202, 2375, 2395, 2403, 2416, 2434, 2460, 2477, 2572, 2607, 2645, 2705, 2750, 2938, 3052, 3102, 3145, 3195, 3201, 3308, 3319, 3327, 3359, 3401, 3432, 3453, 3459, 3483, 3497, 3517, 3526, 3541, 3544, 3647, 3654, 3684, 3706, 3711, 3779, 3788, 3794, 3895, 3981, 3988, 4015, 4060, 4151, 4166, 4229, 4292, 4304, 4354, 4393, 4399, 4426, 4454, 4465, 4487, 4513], 'the': [45, 59, 70, 99, 112, 133, 138, 142, 158, 164, 199, 202, 206, 232, 279, 293, 304, 333, 346, 367, 372, 376, 392, 398, 433, 436, 440, 466, 680, 687, 698, 701, 705, 777, 784, 856, 882, 888, 894, 898, 952, 1067, 1075, 1172, 1206, 1280, 1312, 1356, 1363, 1374, 1456, 1465, 1550, 1554, 1558, 1566, 1570, 1621, 1642, 1645, 1659, 1738, 1745, 1812, 1821, 1825, 1832, 1847, 1890, 1943, 1949, 2000, 2034, 2046, 2075, 2107, 2112, 2118, 2137, 2141, 2150, 2162, 2166, 2197, 2349, 2367, 2425, 2440, 2453, 2600, 2637, 2743, 2774, 2795, 3049, 3100, 3110, 3178, 3187, 3198, 3202, 3272, 3287, 3306, 3315, 3320, 3325, 3328, 3357, 3369, 3399, 3405, 3429, 3433, 3438, 3447, 3451, 3467, 3480, 3494, 3504, 3513, 3524, 3527, 3531, 3539, 3545, 3603, 3631, 3645, 3652, 3658, 3685, 3689, 3733, 3777, 3786, 3946, 3986, 3994, 4013, 4053, 4066, 4137, 4148, 4156, 4162, 4167, 4177, 4188, 4267, 4287, 4301, 4343, 4352, 4391, 4397, 4424, 4468, 4488, 4492, 4502, 4511], 'domains': [46, 280, 799, 1207, 2041], 'mediate': [50, 211, 284, 445, 618, 1852, 4491], 'receptor': [51, 285, 960, 1082, 1174, 1315, 1445, 1450, 1460, 1543, 1559, 1953, 2004, 2049, 3274, 3435, 3809, 3900, 3903, 3914, 3977], 'association': [52, 286], 'is': [53, 93, 287, 327, 669, 786, 1501, 1507, 1626, 1658, 1716, 2111, 3297, 3312, 3446, 3740, 3999, 4056, 4173, 4271, 4447], 'critical': [55, 289, 1208], 'importance': [56, 290], 'understanding': [58, 292], 'function': [60, 294, 770], 'GPCR': [62, 296, 971, 1034, 1614, 1661, 1819], 'oligomers.': [63, 297], 'Using': [64, 298, 2144], 'disulfide-trapping': [66, 300, 1317, 3208, 4311], 'strategy': [67, 301, 4497], 'probe': [69, 303], 'intermolecular': [71, 305], 'contact': [72, 306, 2181, 2187, 3303], 'surfaces,': [73, 307], 'we': [74, 117, 218, 308, 351, 452, 1454, 1703, 1714, 1723, 1926, 2072, 2191, 3205, 3336, 3351, 3612, 3997, 4004, 4074, 4103, 4309], 'demonstrate': [75, 309, 905, 1869, 1942, 2073, 4135], 'cross-linking': [76, 310, 3000, 3119, 3146, 3712, 3739, 3753, 3771, 4090, 4107, 4272, 4413], 'C5a': [78, 115, 127, 221, 312, 349, 361, 455, 1173, 1500, 1540, 1731, 1833, 1860, 1871, 1893, 1934, 2127, 3203, 3273, 3434, 3789, 3913, 4394, 4408], 'in': [80, 98, 111, 123, 132, 205, 231, 314, 332, 345, 357, 366, 439, 465, 697, 1026, 1274, 1295, 1299, 1304, 1373, 1385, 1444, 1451, 1734, 1742, 1820, 1846, 1877, 2081, 2085, 2106, 2134, 2450, 2557, 2642, 2719, 2747, 2799, 2864, 2880, 2887, 2926, 2970, 2979, 3099, 3139, 3221, 3292, 3348, 3356, 3450, 3466, 3474, 3491, 3565, 3976, 3985, 4020, 4093, 4179, 4184, 4187, 4379, 4440, 4506], 'membranes': [81, 315, 967, 2095, 2775, 2796, 3354, 3370, 3755, 3766, 3795, 3867, 3897, 4094, 4314, 4321, 4330, 4402], 'prepared': [82, 316, 2096, 2253, 2514, 3355, 4323], 'from': [83, 317, 704, 2097, 2215, 2266, 2274, 2292, 2303, 2317, 2326, 2333, 2508, 3004, 3553, 3804, 3865, 3904, 4296, 4316], 'both': [84, 318, 1474, 3731, 4081, 4096], 'human': [85, 319, 1859, 2082, 4317], 'neutrophils': [86, 320, 2083], 'stably': [88, 322], 'transfected': [89, 323], 'mammalian': [90, 324, 4480], 'cells': [91, 325, 2099, 2363, 2435, 2441, 2482, 2551, 2566, 2601, 2617], 'mediated': [94, 328], 'cysteine': [97, 131, 153, 204, 331, 365, 387, 438, 1353, 2105, 2115, 2132, 3290], 'second': [100, 207, 334, 441, 1826, 2108, 2790], 'intracellular': [101, 125, 135, 150, 208, 335, 359, 369, 384, 442, 499, 548, 587, 2109, 2138], 'loop.': [102, 336], 'To': [103, 337, 3332, 3600, 3744, 4050, 4389, 4484], 'explore': [104, 338, 1931], 'other': [105, 124, 339, 358, 1431, 1563, 2312, 3970, 4274], 'surfaces': [106, 340], 'might': [108, 342], 'be': [109, 343, 1424, 3644], 'involved': [110, 344, 3564], 'oligomerization': [113, 347, 1035, 1210, 1615, 1891, 1932, 2203, 3995, 4302, 4446], 'receptors,': [116, 350, 783, 964, 4102], 'constructed': [118, 352], 'with': [120, 129, 354, 363, 1549, 1569, 1718, 1863, 2339, 2400, 2474, 2569, 2604, 2848, 2855, 2976, 2987, 3058, 3114, 3122, 3151, 3158, 3170, 3186, 3242, 3371, 3376, 3411, 3424, 3471, 3667, 3716, 3730, 3760, 3764, 3796, 3848, 3920, 3967, 4273, 4282, 4326, 4347, 4359, 4405], 'individual': [121, 355], 'cysteines': [122, 356, 3562, 4178, 4490, 4505], 'regions.': [126, 360], 'first': [134, 368], 'loop': [136, 151, 209, 370, 385, 443, 2110, 4472], 'carboxyl': [139, 373, 2142], 'terminus': [140, 374, 1552, 1572, 2164, 3431, 4150], 'displayed': [141, 154, 375, 388], 'fastest': [143, 377], 'kinetics': [144, 378], 'dimer': [146, 179, 380, 413, 1435, 2176, 2182, 3283, 3486, 3500, 3605, 3637, 3707, 3720, 4059, 4072, 4170], 'formation,': [147, 381, 3568, 4073], 'whereas': [148, 382, 4375], 'an': [149, 383, 572, 1386, 2935, 3425, 3472, 3617, 3663, 3750, 4380], '3': [152, 386, 3742], 'minimal': [155, 389], 'cross-linking.': [156, 390, 1364, 2120], 'Since': [157, 391], 'rate': [159, 393, 3307], 'disulfide': [161, 395, 1928, 3253, 3309, 3528, 3566, 3703, 4400, 4474, 4494], 'trapping': [162, 396, 1929, 1937, 3296], 'reflects': [163, 397], 'proximity': [165, 399, 1068, 3316], 'sulfhydryl': [167, 401, 3245], 'groups,': [168, 402], 'assuming': [169, 403], 'similar': [170, 404, 1388, 1434, 1628, 3576, 3614, 3626, 4285], 'accessibility': [171, 405, 3318], 'flexibility,': [173, 407], 'these': [174, 216, 408, 450, 912, 2123, 2145, 2194, 3968, 4263, 4514], 'results': [175, 409, 696, 1025, 2084, 2169, 2195, 3627, 4264, 4431], 'imply': [176, 410], 'symmetric': [178, 412, 1377, 2175], 'interface': [180, 414, 3288], 'may': [182, 416, 3279, 3974], 'involve': [183, 417], 'either': [184, 418, 1058, 2171, 2803], 'transmembrane': [185, 419, 502, 551, 577, 1746, 1829, 2040], 'helices': [186, 420], '1': [187, 421, 2469, 2489, 2570, 2590, 2643, 2653, 2716, 2748, 3029], '2': [189, 423, 2384, 2605, 4473], 'helix': [191, 425], '4.': [192, 426], 'However,': [193, 427, 4219], 'neither': [194, 428], 'model': [195, 429, 1449, 1548, 1611, 1710, 2201], 'can': [196, 430, 710, 973, 3240, 3520, 3857, 3901, 4240], 'account': [197, 431], 'ability': [200, 434, 3787, 3980, 4014, 4512], 'native': [203, 437, 1720, 2114, 3353, 4442, 4489], 'efficient': [212, 446], 'crosslinking.': [213, 447], 'Based': [214, 448], 'on': [215, 449, 686, 949, 1355, 1858, 2702, 2860, 3418, 3515, 3523, 3534, 3560, 3752, 4012, 4313, 4396, 4423], 'observations,': [217, 451], 'propose': [219, 453], 'higher': [224, 458, 2206], 'order': [225, 459, 2207], 'oligomers': [226, 460, 915, 1874, 4439], '(i.e.': [227, 461], 'tetramers)': [228, 462], 'clusters': [230, 464], 'membrane.': [233, 467], '(GPCRs)': [471], '1The': [472], 'abbreviations': [473, 522], 'used': [474, 523, 1940, 3352], 'are:': [475, 524], 'GPCR,': [476, 525], 'receptor;': [479, 513, 518, 528, 562, 567], 'FRET,': [480, 529], 'fluorescence': [481, 530, 1059], 'resonance': [482, 487, 531, 536], 'energy': [483, 488, 532, 537, 1054], 'transfer;': [484, 489, 533, 538], 'BRET,': [485, 534], 'bioluminescence': [486, 535, 1062], 'YFP,': [490, 539], 'yellow': [491, 540], 'fluorescent': [492, 496, 541, 545, 1867], 'protein;': [493, 497, 542, 546], 'GFP,': [494, 543], 'green': [495, 544], 'IC,': [498, 547], 'loop;': [500, 549], 'TM,': [501, 550], 'helix;': [503, 552], 'CHO,': [504, 553], 'Chinese': [505, 554], 'hamster': [506, 555], 'ovary;': [507, 556], 'CuP,': [508, 557], 'cupric': [509, 558, 1326, 3213, 3276, 3379, 3454, 3677, 4337, 4355], 'orthophenathroline;': [510, 559], 'D2R,': [511, 560], 'D2': [512, 561, 1314, 1370, 2003], 'C5aR,': [514, 563, 2101], 'complement': [515, 564, 1457, 1510], '5a': [517, 566, 1459], 'PBS,': [519, 568, 2576], 'phosphate-buffered': [520, 569, 2478], 'saline.1The': [521], 'saline.': [570, 2856], 'are': [571, 580, 638, 1302, 1652, 3699, 4192], 'evolutionarily': [573], 'conserved': [574, 4469], 'family': [575, 890, 900, 954, 1077, 1282, 1467], 'characterized': [581], 'seven-transmembrane': [583], 'helixes': [584], 'connected': [585], 'loops': [588, 592, 1649, 2139], '(ICs)': [589], 'extracellular': [591, 798, 1357, 1648], '(1Gether': [593], 'U.': [594, 1116, 1963], 'Endocr.': [595], 'Rev.': [596, 745, 919], '2000;': [597, 844, 868, 1126, 1694, 1795], '21:': [598], '90-113Crossref': [599], 'PubMed': [600, 612, 662, 735, 751, 820, 847, 876, 924, 942, 1000, 1013, 1047, 1104, 1134, 1166, 1198, 1232, 1260, 1345, 1417, 1495, 1533, 1603, 1697, 1777, 1803, 1918, 1969, 1996, 2022, 2064, 2544, 2839, 3017, 3265, 3597, 3820, 3838, 3885, 3939, 3963, 4045, 4214, 4257], 'Scopus': [601, 613, 663, 736, 752, 821, 848, 877, 925, 943, 1001, 1014, 1048, 1105, 1135, 1167, 1199, 1233, 1261, 1346, 1418, 1496, 1534, 1698, 1778, 1804, 1919, 1970, 2023, 2065, 2545, 3018, 3266, 3839, 3886, 3940, 4046, 4215, 4258], '(1002)': [602], 'Google': [603, 615, 665, 738, 754, 823, 850, 879, 927, 945, 1003, 1016, 1050, 1107, 1137, 1169, 1201, 1235, 1263, 1348, 1420, 1498, 1536, 1604, 1700, 1780, 1806, 1921, 1972, 1997, 2025, 2067, 2250, 2547, 2840, 3020, 3268, 3598, 3821, 3841, 3888, 3942, 3964, 4048, 4217, 4260], 'Scholar,': [604, 739, 824, 928, 1004, 1236, 1781, 3822], '2Wess': [605], 'J.': [606, 608, 724, 809, 1093, 1123, 1155, 1187, 1249, 1334, 1411, 1484, 1528, 1594, 1757, 1766, 1792, 1907, 1987, 2011, 2059, 2245, 2533, 2830, 3259, 3588, 3816, 3827, 3881, 3908, 3928, 3959, 4034], 'FASEB': [607], '1997;': [609, 748], '11:': [610], '346-354Crossref': [611], '(506)': [614], 'Scholar).': [616, 666, 755, 880, 946, 1017, 1051, 1202, 1349, 1421, 1499, 1537, 1605, 1807, 1922, 2068, 2548, 2841, 3021, 3269, 3599, 3889, 4049, 4218, 4261], 'multitude': [620], 'physiologic': [622, 1037], 'responses': [623], 'serve': [625], 'common': [627, 4476], 'pharmacological': [628, 635], 'targets;': [629], 'more': [630, 883, 3636], 'than': [631, 884], 'half': [632], 'all': [634, 3570, 4479], 'agents': [636, 3107], 'currently': [639], 'prescribed': [640], 'target': [641], '(3Leurs': [643], 'R.': [644, 1118, 2240, 3877, 3879], 'Smit': [645], 'M.J.': [646, 3955], 'Alewijnse': [647], 'A.E.': [648], 'Timmerman': [649], 'H.': [650, 840, 1591, 1677], 'Trends': [651], 'Biochem.': [652, 993, 3815, 3958], 'Sci.': [653, 1962], '1998;': [654, 727, 4206], '23:': [655], '418-422Abstract': [656], 'Full': [657, 659, 730, 732, 815, 817, 871, 873, 1099, 1101, 1129, 1131, 1161, 1163, 1193, 1195, 1255, 1257, 1340, 1342, 1490, 1492, 1600, 1772, 1774, 1798, 1800, 1913, 1915, 1993, 2017, 2019, 2539, 2541, 2836, 3594, 3833, 3835, 3934, 3936, 4040, 4042, 4209, 4211], 'Text': [658, 660, 731, 733, 816, 818, 872, 874, 1100, 1102, 1130, 1132, 1162, 1164, 1194, 1196, 1256, 1258, 1341, 1343, 1491, 1493, 1601, 1773, 1775, 1799, 1801, 1914, 1916, 1994, 2018, 2020, 2540, 2542, 2837, 3595, 3834, 3836, 3935, 3937, 4041, 4043, 4210, 4212], 'PDF': [661, 734, 819, 875, 1103, 1133, 1165, 1197, 1259, 1344, 1494, 1602, 1776, 1802, 1917, 1995, 2021, 2543, 2838, 3596, 3837, 3938, 4044, 4213], '(132)': [664], 'Receptor': [667], 'accomplished': [670], 'remarkably': [673], 'diverse': [674], 'group': [675], 'ligands': [677], 'catalyze': [679], 'exchange': [681, 695], 'GTP': [683], 'GDP': [685], 'α': [688], 'subunit': [689, 703], 'This': [694, 3231], 'dissociation': [699], 'α-GTP': [702], 'βγ': [706], 'dimer.': [707, 2188], 'Both': [708], 'complexes': [709, 3573], 'then': [711, 2472, 2773, 3093, 3366, 4332], 'activate': [712], 'downstream': [713], 'targets,': [714], 'such': [715, 795, 957, 1514, 2044], 'effector': [717], 'enzymes': [718], 'ion': [720], 'channels': [721], '(4Hamm': [722], 'H.E.': [723], 'Biol.': [725, 810, 1094, 1124, 1156, 1188, 1250, 1335, 1413, 1485, 1595, 1767, 1793, 1908, 1988, 2012, 2534, 2831, 3261, 3589, 3828, 3929, 4035], 'Chem.': [726, 811, 1095, 1125, 1157, 1189, 1251, 1336, 1486, 1596, 1768, 1794, 1909, 1989, 2013, 2535, 2832, 3590, 3829, 3930, 4036], '273:': [728], '669-672Abstract': [729], '(929)': [737], '5Clapham': [740], 'D.E.': [741, 1958, 3012, 3587], 'Neer': [742], 'E.J.': [743], 'Annu.': [744], 'Pharmacol.': [746, 937], 'Toxicol.': [747], '37:': [749], '167-203Crossref': [750], '(697)': [753], 'Despite': [756, 1203], 'numerous': [757], 'structure-function': [758, 1728], 'many': [761, 1072], 'different': [762], 'GPCRs,': [763, 902, 956, 1079, 2043], 'fundamental': [765], 'question': [766], 'remains:': [767], 'Do': [768], 'monomers': [772, 1070], 'oligomers?': [775], 'For': [776, 881, 3537], '∼25': [778], 'members': [779, 886, 896, 950, 1073, 1278], 'class': [781], 'C': [782], 'answer': [785], 'clear.': [787], 'These': [788, 1808, 2168, 4117, 4133, 4430], 'dimers': [791, 1947, 3698], 'through': [792, 2667, 3234], 'specialized': [793], 'disulfide-bonded': [797], '(metabotropic': [800], 'glutamate': [801], 'receptors)': [802, 860], '(6Romano': [803], 'C.': [804, 1525], 'Yang': [805], 'W.L.': [806], "O'Malley": [807], 'K.L.': [808], '1996;': [812, 997, 3830, 3882], '271:': [813, 3831], '28612-28616Abstract': [814], '(438)': [822], '7Kunishima': [825], 'N.': [826, 1526], 'Shimada': [827], 'Y.': [828, 830, 1219, 1238], 'Tsuji': [829], 'Sato': [831], 'T.': [832, 836, 1671, 1673, 1686, 1761, 2526, 3925], 'Yamamoto': [833, 1689], 'M.': [834, 862, 917, 1092, 1112, 1142, 1690, 1692, 3875, 3951], 'Kumasaka': [835, 1670], 'Nakanishi': [837], 'S.': [838, 1088, 1148, 1150, 1221, 1242, 1481, 1964, 2827], 'Jingami': [839], 'Morikawa': [841], 'K.': [842, 1227, 1246, 1669, 4200], 'Nature.': [843, 1009, 1043, 1228], '407:': [845], '971-977Crossref': [846], '(1084)': [849], 'Scholar)': [851, 1264, 3943], 'coiled-coil': [853], 'interactions': [854, 3966], 'carboxyl-terminal': [857], 'tails': [858], '(GABAB': [859], '(8Margeta-Mitrovic': [861], 'Jan': [863, 865], 'Y.N.': [864], 'L.Y.': [866], 'Neuron.': [867], '27:': [869], '97-106Abstract': [870], '(569)': [878], '500': [885, 2414], 'rhodopsin': [889, 953, 1076, 1281, 1293, 1400, 1466, 1657, 1822, 1848], '60': [895], 'secretin': [899], 'now': [904], 'rapidly': [908], 'increasing': [909], 'number': [910], '(9Bouvier': [916], 'Nat.': [918], 'Neurosci.': [920], '2001;': [921, 939, 1158, 1414, 3817], '2:': [922], '274-286Crossref': [923], '(577)': [926], '10Rios': [929], 'C.D.': [930], 'Jordan': [931], 'B.A.': [932, 1006, 1040, 1679, 1955], 'Gomes': [933], 'I.': [934, 1682], 'Devi': [935, 1007, 1041], 'L.A.': [936, 1008, 1042], 'Ther.': [938], '92:': [940], '71-87Crossref': [941], '(282)': [944], 'Early': [947], 'work': [948], 'dopamine': [959, 1313, 1369, 2002], 'subtypes': [961], 'opioid': [963, 1020, 1139], 'utilized': [965, 3206], 'detergent-solubilized': [966], 'show': [969], 'molecules': [972, 1294, 2080, 4017, 4239, 4276, 4436], 'co-immunoprecipitate': [974], 'homodimers': [976], 'heterodimers': [978], '(11Ng': [979], 'G.Y.': [980], "O'Dowd": [981], 'B.F.': [982], 'Lee': [983], 'S.P.': [984, 1180, 1763, 1900, 2518, 4027], 'Chung': [985], 'H.T.': [986], 'Brann': [987], 'M.R.': [988, 1982], 'Seeman': [989], 'P.': [990, 1751, 3871], 'George': [991], 'S.R.': [992], 'Biophys.': [994], 'Res.': [995], 'Commun.': [996], '227:': [998], '200-204Crossref': [999], '(240)': [1002], '12Jordan': [1005], '1999;': [1010, 1044, 1769, 2536], '399:': [1011, 1045], '697-700Crossref': [1012, 1046], '(958)': [1015, 1049], 'Heterodimerization': [1018], 'κ': [1022], 'δ': [1024], 'novel': [1027], 'ligand': [1028, 1555, 1567, 1577, 4395, 4451], 'binding': [1029, 1547], 'function,': [1031], 'proving': [1032], 'relevance': [1038], '(12Jordan': [1039], 'More': [1052], 'recently,': [1053], 'transfer': [1055], 'studies,': [1056], 'using': [1057, 1287, 2093, 2263, 2345, 3177, 3616], '(FRET)': [1060], '(BRET),': [1063], 'have': [1064, 1265, 1704, 1724, 3749, 3757], 'also': [1065, 2030, 4265], 'shown': [1066, 3800, 3917, 4236], 'including': [1080], 'β2-adrenergic': [1081], '(13Mercier': [1083], 'J.F.': [1084], 'Salahpour': [1085], 'A.': [1086, 1090, 1178, 1225, 1248, 1402, 1783, 1898, 1965, 3869, 3873, 4025], 'Angers': [1087], 'Breit': [1089], 'Bouvier': [1091], '2002;': [1096, 2061, 4254], '277:': [1097], '44925-44931Abstract': [1098], '(416)': [1106], 'Scholar),': [1108, 1138, 1170, 1973, 1998, 2251, 3842], 'somatostatin': [1109], '(14Rocheville': [1111], 'Lange': [1113], 'D.C.': [1114, 1684], 'Kumar': [1115], 'Sasi': [1117], 'Patel': [1119, 1121], 'R.C.': [1120], 'Y.C.': [1122], '275:': [1127, 1796], '7862-7869Abstract': [1128], '(459)': [1136], '(15McVey': [1141], 'Ramsay': [1143], 'D.': [1144, 1217, 1240], 'Kellett': [1145], 'E.': [1146, 1950], 'Rees': [1147], 'Wilson': [1149], 'Pope': [1151], 'A.J.': [1152], 'Milligan': [1153], 'G.': [1154, 1583], '276:': [1159], '14092-14099Abstract': [1160], '(226)': [1168], '(57Floyd': [1175, 1895, 4022], 'D.H.': [1176, 1896, 4023], 'Geva': [1177, 1897, 4024], 'Bruinsma': [1179, 1899, 4026], 'Overton': [1181, 1901, 4028], 'M.C.': [1182, 1902, 4029], 'Blumer': [1183, 1903, 4030], 'K.J.': [1184, 1904, 4031], 'Baranski': [1185, 1790, 1905, 2521, 4032], 'T.J.': [1186, 1749, 1791, 1906, 2522, 4033], '2003;': [1190, 1229, 1252, 1337, 1530, 1910, 2014, 3931, 3960, 4037], '278:': [1191, 1253, 1338, 1911, 2015, 3932, 4038], '35354-35361Abstract': [1192, 1912, 4039], '(55)': [1200, 1920, 4047], 'intense': [1204], 'investigation,': [1205], 'remain': [1211], 'elusive.': [1212], 'Recently,': [1213], 'two': [1214, 1277, 1617, 2173, 4142, 4464], 'works': [1215], '(16Fotiadis': [1216], 'Liang': [1218], 'Filipek': [1220, 1241], 'Saperstein': [1222, 1243], 'D.A.': [1223, 1244, 4246], 'Engel': [1224, 1247], 'Palczewski': [1226, 1245], '421:': [1230], '127-128Crossref': [1231], '(652)': [1234], '17Liang': [1237], 'Fotiadis': [1239], '21655-21662Abstract': [1254], '(543)': [1262], 'proposed': [1266], 'TM': [1268, 1360, 1382, 2179, 2185, 4470], '4': [1269, 1361, 1383, 2186, 2445, 2580, 2736, 2767, 2786, 2924], 'plays': [1270, 1443], 'vital': [1272], 'role': [1273, 1441], 'homodimerization': [1275], 'GPCRs.': [1284], 'Elegant': [1285], 'atomic': [1288], 'force': [1289], 'microscopy': [1290, 1394], 'showed': [1291], 'rod': [1296], 'outer': [1297], 'segments': [1298, 1747, 1830], 'mouse': [1300], 'retinas': [1301], 'arranged': [1303], 'orderly': [1305], 'rows': [1306], 'dimers.': [1308, 2087], 'In': [1309, 1824, 1923, 2069, 2189, 3210, 3624, 4351], '(D2R),': [1316], 'experiments': [1318, 3615], 'demonstrated': [1319, 3679, 3709, 4005], 'crosslinking': [1320, 2155, 2806, 4157], 'when': [1323, 1875, 4145], 'exposed': [1324, 2804, 4333], 'orthophenanthroline': [1327, 3214, 3277, 3380, 3455, 3678, 4338, 4356], '(18Guo': [1328, 2005], 'W.': [1329, 2006, 2238], 'Shi': [1330, 2007], 'L.': [1331, 2008, 3923, 3953], 'Javitch': [1332, 2009], 'J.A.': [1333, 1580, 2010, 2234], '4385-4388Abstract': [1339, 2016], '(265)': [1347, 2024], 'Moreover,': [1350, 1855, 4003], 'single': [1352, 2131], 'substitution': [1354], 'side': [1358], 'abolished': [1362], 'authors': [1366], 'conclude': [1367], 'reside': [1372], 'membrane': [1375, 3046, 3781, 3972, 3990, 4376], 'dimers,': [1378], 'oriented': [1379], 'about': [1380], 'interface,': [1384], 'arrangement': [1387, 1945], 'observed': [1391, 2119, 3505, 3604, 4104, 4493], 'electron': [1393], 'crystals': [1397], 'squid': [1399], '(19Davies': [1401], 'Gowen': [1403], 'B.E.': [1404], 'Krebs': [1405], 'A.M.': [1406, 1479], 'Schertler': [1407], 'G.F.': [1408, 1978], 'Saibil': [1409], 'H.R.': [1410, 1765, 2532], 'Mol.': [1412, 3260], '314:': [1415], '455-463Crossref': [1416], '(66)': [1419], 'It': [1422, 4172], 'will': [1423], 'great': [1426], 'interest': [1427], 'determine': [1429, 3745], 'if': [1430, 1438, 3271, 3286], 'utilize': [1433], 'interfaces': [1436, 2158], 'and,': [1437], 'so,': [1439], 'what': [1440], 'dimerization': [1442, 4427], 'function.': [1446], 'As': [1447], 'this': [1452, 1924, 2027, 2070, 2091, 3211, 3334, 3642], 'study,': [1453, 1925, 2071], 'examine': [1455, 3197, 4300, 4390], '(C5aR),': [1461], 'member': [1463], 'activates': [1473], 'Gαi': [1475], 'Gα16': [1477], '(20Buhl': [1478], 'Osawa': [1480], 'Johnson': [1482], 'G.L.': [1483, 1986], '1995;': [1487], '270:': [1488], '19828-19832Abstract': [1489], '(63)': [1497], '74-amino': [1503], 'acid': [1504], 'glycoprotein': [1505], 'liberated': [1508], 'during': [1509], 'mediates': [1513, 2117], 'processes': [1515], 'neutrophil': [1517], 'chemotaxis,': [1518], 'platelet': [1519], 'activation,': [1520], 'vascular': [1522], 'permeability': [1523], '(21Gerard': [1524], 'Engl.': [1527], 'Med.': [1529], '348:': [1531], '167-169Crossref': [1532], '(71)': [1535], 'Binding': [1538], 'its': [1542, 1719], 'involves': [1544], 'two-site': [1546], 'COOH': [1551, 2163, 4149], 'inserting': [1556], 'into': [1557, 2620, 2934], 'interhelical': [1560], 'crevice': [1561], 'portions': [1564], 'interacting': [1568], 'NH2': [1571, 3430], 'C5aR': [1574, 1606, 1625, 1632, 1712, 2079, 2220, 3632, 3659, 3697, 3724, 3899, 3982, 4016, 4082, 4120, 4152, 4169, 4230, 4270, 4275, 4294, 4307, 4361, 4373, 4435, 4460, 4507], 'enhance': [1576], 'affinity': [1578], '(22DeMartino': [1579], 'Van': [1581], 'Riper': [1582], 'Siciliano': [1584, 2826], 'S.J.': [1585, 4204], 'Molineaux': [1586], 'C.J.': [1587], 'Konteatis': [1588], 'Z.D.': [1589], 'Rosen': [1590], 'Springer': [1592, 2828], 'M.S.': [1593, 2829], '1994;': [1597, 1990], '269:': [1598, 1991], '14446-14450Abstract': [1599], 'serves': [1607], 'useful': [1610, 1883, 3299], 'study': [1613, 3302], 'major': [1618], 'reasons.': [1619], 'First,': [1620], 'architecture': [1623], 'very': [1627], 'bovine': [1630, 2323, 2382], 'rhodopsin.': [1631], '350': [1634], 'amino': [1635], 'acids': [1636], 'versus': [1637], '348': [1638], 'rhodopsin,': [1640], 'sizes': [1643], 'intra-': [1646], 'termini': [1651], 'comparable': [1653], '(Fig.': [1654, 3487, 3622, 3638, 3692, 3767, 4115, 4387, 4428, 4482], '1).': [1655, 4483], 'Bovine': [1656], 'only': [1660, 2113], 'whose': [1662], 'crystal': [1663, 4189], 'structure': [1664, 1849, 3200, 4190], 'elucidated': [1667], '(23Palczewski': [1668], 'Hori': [1672], 'Behnke': [1674], 'C.A.': [1675], 'Motoshima': [1676], 'Fox': [1678], 'Le': [1680], 'Trong': [1681], 'Teller': [1683], 'Okada': [1685], 'Stenkamp': [1687], 'R.E.': [1688], 'Miyano': [1691], 'Science.': [1693, 2306, 3013, 4253], '289:': [1695], '739-745Crossref': [1696], '(4970)': [1699], 'Scholar);': [1701, 2026, 3965], 'therefore,': [1702], 'able': [1706], 'generate': [1708], 'believe': [1715, 2192, 3641], 'consistent': [1717], 'structure.': [1721, 3331, 3536], 'Second,': [1722], 'performed': [1725, 2338, 3382, 3613, 4092, 4310], 'high': [1726, 4243], 'throughput': [1727], 'expressed': [1733, 1876, 4306], 'yeast': [1735, 4021], 'map': [1737, 1843], 'most': [1739, 2151], 'essential': [1740, 1835], 'residues': [1741, 2229, 3291, 3321], 'each': [1743, 2135], '(24Baranski': [1748], 'Herzmark': [1750], 'Lichtarge': [1752, 1786, 2523], 'O.': [1753, 1787, 2524], 'Gerber': [1754], 'B.O.': [1755], 'Trueheart': [1756], 'Meng': [1758, 2527], 'E.C.': [1759, 2528], 'Iiri': [1760, 2525], 'Sheikh': [1762], 'Bourne': [1764, 2531], '274:': [1770, 2537], '15757-15765Abstract': [1771], '(121)': [1779], '25Geva': [1782], 'Lassere': [1784], 'T.B.': [1785], 'Pollitt': [1788], 'S.K.': [1789], '35393-35401Abstract': [1797], '(52)': [1805, 3941], 'comprehensive': [1809], 'provide': [1811, 1881, 2196], 'largest': [1813], 'mutational': [1814], 'data': [1815, 4134], 'base': [1816], 'any': [1818, 3412], 'family.': [1823], 'fourth': [1828], 'receptor,': [1834, 3204, 3548], 'residues,': [1836], 'identified': [1837], 'random': [1839], 'saturation': [1840, 3710], 'mutagenesis': [1841, 2336], 'analysis,': [1842, 3212, 3335], 'onto': [1844], 'positions': [1845], 'could': [1851], 'dimer/oligomer': [1853], 'interactions.': [1854], 'FRET': [1856, 4019], 'tagged': [1862], 'YFP': [1864, 3621, 4067, 4138, 4180, 4225], 'cyan': [1866], 'protein': [1868, 3330, 3345, 3949, 4281], 'yeast,': [1878], 'which': [1879, 3561, 3661, 4140, 4466, 4486], 'should': [1880], 'genetic': [1884], 'system': [1885], 'begin': [1887], 'characterize': [1889], 'use': [1927], 'Disulfide': [1936, 2998, 3193, 3295, 4458], 'spatial': [1944], 'coli': [1951], 'aspartate': [1952, 3546], '(26Lynch': [1954], 'Koshland': [1956, 3010, 3585], 'Jr.,': [1957, 3011, 3586], 'Proc.': [1959], 'Natl.': [1960], 'Acad.': [1961], '1991;': [1966], '88:': [1967], '10402-10406Crossref': [1968], '(124)': [1971], 'bacterial': [1974], 'Trg': [1975], 'chemoreceptor': [1976], '(27Lee': [1977], 'Burrows': [1979], 'G.G.': [1980], 'Lebert': [1981], 'Dutton': [1983], 'D.P.': [1984], 'Hazelbauer': [1985], '29920-29927Abstract': [1992], 'above-mentioned': [2001], 'technique': [2028], 'applied': [2032], 'analysis': [2035], 'intramolecular': [2037], 'contacts': [2038], 'between': [2039, 3493], 'M3': [2047], 'muscarinic': [2048], '(28Hamdan': [2050], 'F.F.': [2051], 'Ward': [2052], 'S.D.': [2053, 2236], 'Siddiqui': [2054], 'N.A.': [2055], 'Bloodworth': [2056], 'L.M.': [2057], 'Wess': [2058], 'Biochemistry.': [2060], '41:': [2062], '7647-7658Crossref': [2063], '(33)': [2066], 'oxidation': [2076, 4377], 'endogenous': [2078, 4438], 'disulfide-linked': [2086, 3282, 3518], 'Further': [2088], 'probing': [2089], 'finding,': [2092], 'intact': [2094], 'CHO': [2098, 2510, 2550, 3896], 'expressing': [2100, 3341], 'revealed': [2102, 2156], 'We': [2121, 3640], 'extend': [2122], 'generating': [2126], 'contain': [2130], 'substitutions': [2133], 'terminus.': [2143], 'collisional': [2148], 'probes,': [2149], 'rapid': [2152], 'rates': [2153], 'preferred': [2157], 'near': [2159, 3479], 'IC1': [2160], 'receptor.': [2167], 'support': [2170, 4266], 'potential': [2174], 'models,': [2177], '1,2': [2180], 'addition,': [2190], 'basis': [2198], 'proposes': [2205], 'structures.': [2208], 'Materials—Cupric': [2209], 'sulfate': [2210, 2331], '1,10-phenanthroline': [2212], 'were': [2213, 2315, 2325, 2353, 2364, 2442, 2466, 2483, 2499, 2513, 2552, 2567, 2602, 2618, 2640, 2776, 2797, 2820, 2858, 2877, 2985, 2993, 3084, 3108, 3112, 3147, 3174, 3416, 3563, 3628, 3762, 4322, 4331, 4403], 'obtained': [2214, 2291], 'Sigma.': [2216], 'rabbit': [2218, 2269], 'polyclonal': [2219, 2270], 'amino-terminal': [2221], 'antibody,': [2222, 3660], 'raised': [2223], 'against': [2224], 'peptide': [2226], 'corresponding': [2227, 4370], '9–29': [2230], '(29Morgan': [2231], 'E.L.': [2232], 'Ember': [2233], 'Sanderson': [2235], 'Scholz': [2237], 'Buchner': [2239], 'Ye': [2241], 'R.D.': [2242], 'Hugli': [2243], 'T.E.': [2244, 2825], 'Immunol.': [2246], '1993;': [2247], '151:': [2248], '377-388PubMed': [2249], 'was': [2252, 2273, 2290, 2301, 2332, 2337, 2398, 2410, 2427, 2455, 2463, 2496, 2563, 2597, 2634, 2676, 2687, 2698, 2726, 2740, 2745, 2764, 2846, 2915, 2932, 2944, 2968, 3002, 3043, 3056, 3120, 3381, 3396, 3509, 3606, 3773, 4091, 4221, 4345, 4414, 4498], 'Zymed': [2255], 'Laboratories': [2256], 'Inc.': [2257, 2278], '(San': [2258], 'Francisco,': [2259], 'CA)': [2260], 'affinity-purified': [2262], 'Sulfolink': [2264], 'Gel': [2265, 3180], 'Pierce.': [2267], 'GFP(FL)': [2271], 'antibody': [2272, 3426, 3618, 3633, 4362], 'Santa': [2275], 'Cruz': [2276], 'Biotechnology,': [2277], '(Santa': [2279], 'Cruz,': [2280], 'CA).': [2281, 2296], 'Goat': [2282], 'anti-rabbit': [2283, 3160], 'IgG': [2284, 3161], '(H': [2285, 3162], '+': [2286, 3163], 'L)': [2287, 3164], 'horseradish': [2288, 3165], 'peroxidase': [2289], 'Chemicon': [2293], 'International': [2294], '(Temecula,': [2295], 'Lumi-LightPLUS': [2297, 3171], 'Western': [2298], 'blotting': [2299], 'Substrate': [2300], 'purchased': [2302, 2316], 'Roche': [2304], 'Applied': [2305], 'Prestained': [2307], 'mass': [2309, 4284], 'markers': [2310], 'SDS-PAGE': [2313, 3421, 3516], 'reagents': [2314], 'Bio-Rad.': [2318], "Ham's": [2319, 2377], 'F-12': [2320, 2378], 'fetal': [2322, 2381], 'serum': [2324, 2383], 'BioWhittaker': [2327], '(Walkersville,': [2328], 'MD).': [2329], 'G418': [2330, 2491], 'Invitrogen.': [2334], 'Site-directed': [2335], 'Pfu': [2340], 'Turbo': [2341], 'DNA': [2342, 2397], 'polymerase': [2343], '(Stratagene)': [2344], 'pcDNA3': [2346], 'C5aR-YFP': [2347, 3343, 3439, 3499, 4061, 4086, 4129, 4288], 'template.': [2350], 'All': [2351], 'mutations': [2352], 'confirmed': [2354], 'sequencing.': [2356], 'Stable': [2357], 'Cell': [2358], 'Line': [2359], 'Generation': [2360], 'Maintenance—CHO': [2362], 'split': [2365], '1:15': [2366], 'day': [2368], 'before': [2369, 2386, 2488, 4342], 'transfection': [2370], 'fed': [2372], '9.0': [2373], 'ml': [2374, 2459, 2476, 2571, 2606, 2644, 2704, 2749], 'fresh': [2376], 'containing': [2379, 2392, 2892, 3898, 4095], '10%': [2380, 2461], 'h': [2385, 2446], 'precipitation.': [2387], 'A': [2388], '450-μl': [2389], 'aqueous': [2390], 'solution': [2391, 2574, 2596, 2609, 2675, 2763, 2866, 2891], '40': [2393], 'μg': [2394], 'plasmid': [2396], 'combined': [2399], '50': [2401, 2711, 3087, 3134], 'μl': [2402, 2415], '2.5': [2404, 2759, 2893], 'm': [2405, 2752, 2940, 2989, 3373, 3854, 3892, 4328], 'CaCl2.': [2406], 'DNA/CaCl2': [2408], 'mixture': [2409, 2725, 2914], 'added': [2411, 3044], 'dropwise': [2412], 'HeBS.': [2417], 'After': [2418, 2439, 2788], '20-min': [2420], 'incubation': [2421, 3169], 'at': [2422, 2436, 2447, 2610, 2625, 2678, 2728, 2735, 2766, 2778, 2785, 2811, 2885, 2923, 2946, 2995, 3086, 3126, 3391, 3443, 4109, 4242, 4367, 4384], 'room': [2423, 2611, 3127, 3392], 'temperature,': [2424], 'precipitate': [2426], 'distributed': [2428], 'evenly': [2429], 'over': [2430], '10-cm': [2432, 2559], 'plate': [2433], '75%': [2437], 'confluence.': [2438], 'incubated': [2443, 2603, 2765, 3125], '37': [2448], '°C': [2449, 2768, 2925, 3088, 3135], '5%': [2451], 'CO2,': [2452], 'medium': [2454, 2562], 'removed,': [2456, 2564, 2598], '2.0': [2458], 'Me2SO': [2462], 'added.': [2464, 2497], 'Cells': [2465, 2857, 2876], 'shocked': [2467], 'min': [2470, 2734, 2784, 2922, 3091, 3138, 3390, 3673, 3715, 4341], 'washed': [2473, 2568, 2878], '5': [2475, 2614, 2630, 2683, 3032, 3853, 4406], 'saline': [2479], '(PBS).': [2480], 'allowed': [2484], 'double': [2486], 'twice': [2487], 'mg/ml': [2490], 'and/or': [2492], '250': [2493], 'μg/ml': [2494, 2584, 2587, 2657, 2908, 3072, 3075], 'zeocin': [2495], 'Colonies': [2498], 'selected': [2500], 'expanded': [2502], 'after': [2503, 3118, 3674, 3713], '14': [2504], 'days': [2505], 'removed': [2507, 2699], 'selection.': [2509, 3349], 'Membrane': [2511, 2817, 2991], 'Preparation—Membranes': [2512], 'described': [2516, 2823, 3006], '(30Sheikh': [2517], 'Vilardarga': [2519], 'J.P.': [2520], 'Nissenson': [2529], 'R.A.': [2530], '17033-17041Abstract': [2538], '(182)': [2546, 3887], 'Briefly,': [2549, 2842], 'grown': [2553], '90%': [2555], 'confluence': [2556], 'three': [2558, 3571, 4105, 4168], 'plates,': [2560], 'culture': [2561], 'PBS/EDTA': [2573, 2608, 2633], '(1×': [2575], '10': [2577, 2671, 2870, 2907, 2951, 2971, 3071, 3074, 3090, 3137], 'mm': [2578, 2581, 2591, 2649, 2654, 2660, 2709, 2712, 2717, 2755, 2760, 2868, 2871, 2874, 2894, 2903, 2972, 3027, 3030, 3033, 3036, 3041, 3066, 3069, 3079, 3116, 3378, 3669, 4336], 'EDTA,': [2579, 2655, 3067], 'EGTA,': [2582], '20': [2583, 2586, 2656, 2921, 2957, 2977, 3065, 3068], 'leupeptin,': [2585, 2910, 3076], 'aprotinin,': [2588, 2658, 2909, 3073], 'phenylmethylsulfonyl': [2592, 2661, 2904, 3080], 'fluoride).': [2593, 3081], 'wash': [2595, 2792], 'temperature': [2612, 3128], 'min.': [2615, 2631, 2684, 2965, 3743], 'harvested': [2619], '2-ml': [2621], 'tubes': [2622, 2722], 'centrifuged': [2624, 2677, 2694, 2727, 2945], '960': [2626, 2679], '×': [2627, 2680, 2730, 2780, 2948, 2954, 2961], 'g': [2628, 2681, 2731, 2781, 2949, 2955, 2962], 'aspirated,': [2635, 2741], 'cell': [2638, 3339, 4077], 'pellets': [2639], 'resuspended': [2641, 2746, 2798, 2863, 2884, 2969], 'lysis': [2646, 2865, 2881], 'buffer': [2647, 2801, 2882, 3023, 3062], '(50': [2648], 'Tris,': [2650, 2713, 3037], 'pH': [2651, 2714, 2757, 3038], '7.5,': [2652, 2715], '0.5': [2659, 2939, 3078], 'fluoride)': [2662], 'homogenized': [2664, 2975], 'aspirating': [2666], '27-gauge': [2669], 'needle': [2670], 'times.': [2672], 'lysed': [2674], 'supernatant': [2686, 2697, 2739], 'transferred': [2688], 'new': [2691], 'tube': [2692], 'again.': [2695], 'overlaid': [2701], '1.5': [2703, 3026, 3377, 4335], 'sucrose': [2706, 2800], 'cushion': [2707], '(250': [2708], 'sucrose,': [2710], 'EDTA)': [2718], 'TLA100.3': [2720], 'centrifuge': [2721], '(Sorvall).': [2723], '217,000': [2729, 2779], '30': [2733, 2770, 2783, 3130, 3672, 4410], '°C.': [2737, 2787, 2813, 2997], 'pellet': [2744, 2967], '6': [2751, 2988, 3372, 3891, 4327], 'urea': [2753, 2791, 3761, 3797, 3855, 3893, 4007], '(10': [2754], 'HEPES,': [2756], '7.3,': [2758], 'EGTA).': [2761], 'min,': [2771, 2952, 2958, 3131, 4411], 'pelleted': [2777], 'sedimentation,': [2794], 'frozen': [2808], 'stored': [2810, 2994], '–80': [2812, 2996], 'Neutrophil': [2814], 'Isolation': [2815], 'Preparation—Polymorphonuclear': [2818], 'leukocytes': [2819], 'isolated': [2821, 4315], '(31Rollins': [2824], '1988;': [2833, 3591], '263:': [2834, 3592], '520-526Abstract': [2835], 'blood': [2843], '(20': [2844], 'ml)': [2845], 'treated': [2847, 2986, 3113, 3759, 4325, 4404], '25': [2849], 'units': [2850], 'heparin': [2851], 'diluted': [2853], '1:1': [2854], 'separated': [2859], 'Ficoll-Hypaque': [2861], '(150': [2867], 'NH4Cl,': [2869], 'KHCO3,': [2872], '90': [2873], 'EDTA).': [2875], 'once': [2879], '107/ml': [2886], "Hanks'": [2888], 'balanced': [2889], 'salt': [2890], 'CaCl2,': [2895, 3031], '100': [2896, 3040], 'units/ml': [2897], 'DNase': [2898], 'I,': [2899], '0.1%': [2900], 'gelatin,': [2901], '0.1': [2902], 'fluoride,': [2905], 'chymostatin.': [2912], 'equilibrated': [2916], '400': [2918, 2947], 'p.s.i.': [2919], 'nitrogen': [2928], 'bomb.': [2929], 'bomb': [2931], 'evacuated': [2933], 'equal': [2936], 'volume': [2937], 'KHCO3.': [2941], 'material': [2943], '104': [2953], '105': [2960], '45': [2964, 4368], 'HEPES': [2973], 'strokes': [2978], 'ground': [2981], 'glass': [2982], 'homogenizer.': [2983], 'Membranes': [2984], 'urea.': [2990, 3374], 'aliquots': [2992], 'Trapping—The': [2999], 'procedure': [3001], 'modified': [3003], 'previously': [3007], '(32Falke': [3008], 'J.J.': [3009, 3258], '1987;': [3014], '237:': [3015], '1596-1600Crossref': [3016], '(228)': [3019], 'Cross-linking': [3022, 4291], '(final': [3024], 'concentrations': [3025, 4244], 'Cu(II)-(o-phenanthroline)3,': [3028], 'Mg2+,': [3034], '16.7': [3035], '8.0,': [3039], 'NaCl)': [3042], 'preparations': [3047], 'desired': [3050], 'amount': [3051, 3653], 'time.': [3053], 'reaction': [3055, 3223, 3232, 3395, 4344], 'terminated': [3057, 3397, 4346], '2×': [3059], 'SDS': [3060, 3140], 'sample': [3061, 3141], '(4%': [3063], 'SDS,': [3064], 'N-ethylmaleimide,': [3070, 3123], 'samples': [3083, 3111], 'heated': [3085], 'resolved': [3094, 3417], 'SDS-polyacrylamide': [3096], 'gel': [3097, 3581], 'electrophoresis': [3098], 'absence': [3101, 3358, 3452, 3987, 4353], 'reducing': [3103, 3106], 'agents.': [3104], 'If': [3105], 'used,': [3109], '200': [3115, 3668], 'dithiothreitol,': [3117], 'quenched': [3121], 'followed': [3132, 3167], 'buffer.': [3142], 'effects': [3144, 3533], 'examined': [3148], 'immunoblotting': [3150, 4358], 'anti-C5aR': [3152], 'anti-GFP': [3154], 'antibodies': [3155, 3732], 'treatment': [3157, 3278, 3856, 3894, 4008, 4417], 'goat': [3159], 'peroxidase,': [3166], 'substrate.': [3172], 'Images': [3173], 'acquired': [3175], 'ChemiDoc': [3179], 'Documentation': [3181], 'System': [3182], '(Bio-Rad)': [3183], 'analyzed': [3185], 'program': [3188], 'Quantity': [3189], 'One': [3190], '(version': [3191], '4).': [3192], 'Trapping': [3194], 'C5aR—To': [3196], 'oligomer': [3199, 3285], 'strategy.': [3209], '(CuP)': [3215], 'acts': [3216], 'redox': [3219], 'catalyst': [3220], 'uses': [3225], 'dioxygen': [3226], 'oxidize': [3228], 'free': [3229, 3244, 3413], 'sulfhydryls.': [3230, 3414], 'proceeds': [3233], 'sulfur': [3236, 3248], 'radical': [3237, 3249], 'intermediate': [3238], 'collide': [3241], 'another': [3247], 'bond': [3254, 3310, 3529, 3567], '(33Careaga': [3255], 'C.L.': [3256], 'Falke': [3257], '1992;': [3262], '226:': [3263], '1219-1235Crossref': [3264], '(216)': [3267], 'Therefore,': [3270], 'oligomerizes,': [3275], 'produce': [3280], 'close': [3293], 'proximity.': [3294], 'method': [3300], 'interfaces,': [3304], 'because': [3305], 'formation': [3311, 3708, 3721], 'dependent': [3313], 'upon': [3314], 'well': [3323], 'flexibility': [3326], 'local': [3329], 'facilitate': [3333], 'generated': [3337, 4075], 'stable': [3338, 4076], 'lines': [3340, 4078], 'fusion': [3344, 4289], 'aid': [3347, 3975], 'Also,': [3350, 3852], 'detergents': [3360], 'remove': [3362, 3858], 'detergent-induced': [3363], 'artifacts': [3364], 'subsequently': [3367], 'stripped': [3368], 'Crosslinking': [3375], '1,': [3384], '2,': [3385], '4,': [3386], '8,': [3387], '16': [3389, 3714, 4340], 'temperature.': [3393], 'addition': [3400, 3458], 'EDTA': [3402], 'chelate': [3404], 'copper': [3406], 'N-ethylmaleimide': [3408, 4348], 'react': [3410], 'Reactions': [3415], 'nonreducing': [3420], 'gel.': [3422], 'Probing': [3423], 'recognizes': [3428, 3620, 3634, 3662], 'shows': [3436, 3462], 'monomer': [3440, 3655, 3691], 'migrates': [3442], '80': [3444], 'kDa': [3445, 3555, 4114, 4369, 4386], 'predominant': [3448, 4106], 'species': [3449, 3469, 3687, 4055, 4382], 'treatment.': [3456], 'oxidizing': [3460], 'agent': [3461], 'time-dependent': [3464], 'decrease': [3465], '80-kDa': [3468, 3690], 'coupled': [3470], 'increase': [3473], '180-kDa': [3476, 3686, 4054], 'immunoreactive': [3477], 'species,': [3478], 'expected': [3481, 3495], 'size': [3482, 3492, 3577], 'C5aRYFP': [3485], '2A).': [3488], 'difference': [3490], 'mobility': [3496, 3506, 3514, 3540], '(160': [3501], 'kDa)': [3502, 3508, 4084], '(180': [3507, 4131], 'not': [3510, 3607, 3784, 4069, 4154, 4174, 4182, 4193, 4278, 4448], 'unexpected,': [3511], 'since': [3512, 4186, 4232], 'proteins': [3519, 3748, 3782, 3806, 3973, 3991], 'vary': [3521], 'depending': [3522, 3559], 'location': [3525], 'resultant': [3532], 'secondary': [3535], 'example,': [3538], 'forms': [3543], '60-kDa': [3550], 'monomer,': [3551, 4374], 'varied': [3552], '120–150': [3554], '180': [3557, 4113], 'kDa,': [3558], 'yet': [3569, 3729, 3807], 'had': [3574, 4009], 'determined': [3579], 'filtration': [3582], '(34Milligan': [3583], 'D.L.': [3584], '6268-6275Abstract': [3593], 'ensure': [3601, 4064], 'affected': [3608], 'epitope': [3610], 'masking,': [3611], '2B).': [3623], 'general,': [3625], 'obtained,': [3629, 3774], 'but': [3630], 'proportionally': [3635], '2D).': [3639], 'result': [3646], 'NH2-terminal': [3648, 3664], 'proteolysis': [3649], 'reduces': [3651], 'detected': [3656, 3722], 'epitope.': [3665], 'Treatment': [3666, 3793], 'dithiothreitol': [3670], 'exposure': [3675], 'nearly': [3681, 4163, 4478], 'complete': [3682], 'collapse': [3683], '2C),': [3693], 'thus': [3694, 3843], 'demonstrating': [3695], 'linked': [3700], 'bond.': [3704], 'Quantification': [3705], '75': [3717], '50%': [3719], 'GFP': [3726, 4238], 'immunoblotting,': [3727], 'respectively,': [3728], 'time': [3734], 'required': [3735], 'reach': [3737], 'half-maximal': [3738], 'approximately': [3741], 'whether': [3746], 'accessory': [3747], 'effect': [3751, 4011, 4392, 4422], 'efficiency,': [3754], 'compared': [3763], 'untreated': [3765], '3A).': [3768], 'An': [3769], 'identical': [3770], 'pattern': [3772], 'indicating': [3775], 'presence': [3778], 'peripheral': [3780, 3971, 3989], 'does': [3783, 4068, 4153], 'influence': [3785, 4071, 4155], 'cross-link.': [3792], 'uncouple': [3802, 3902], 'maintain': [3808], 'activity': [3810], '(35Lim': [3811], 'W.K.': [3812], 'Neubig': [3813], 'R.R.': [3814], '354:': [3818], '337-344Crossref': [3819], '36Hartman': [3823], 'J.I.': [3824], 'Northup': [3825], 'J.K.': [3826], '22591-22597Abstract': [3832], '(85)': [3840], 'allowing': [3844], 'functional': [3846], 'reconstitution': [3847], 'purified': [3849], 'InaD,': [3859], 'PDZ': [3861], 'domain-containing': [3862], 'scaffold': [3863], 'protein,': [3864], 'photoreceptor': [3866], '(37Huber': [3868], 'Sander': [3870], 'Gobert': [3872], 'Bahner': [3874], 'Hermann': [3876], 'Paulsen': [3878], 'EMBO': [3880], '15:': [3883], '7036-7045Crossref': [3884], 'Similarly,': [3890], '2T.': [3907], 'Baranski,': [3909], 'unpublished': [3910], 'observation.': [3911], 'interact': [3919], 'β-arrestins': [3921], '(38Braun': [3922], 'Christophe': [3924], 'Boulay': [3926, 3956], 'F.': [3927, 3957], '4277-4285Abstract': [3933], 'recently': [3945], 'Wiskott-Aldrich': [3947], 'syndrome': [3948], '(39Tardif': [3950], 'Brouchon': [3952], 'Rabiet': [3954], '372:': [3961], '453-463Crossref': [3962], 'oligomerization.': [3978], 'cross-link': [3984], 'argues': [3992], 'observe': [3998], 'receptor-autonomous': [4001], 'process.': [4002], 'little': [4010, 4419], 'prove': [4051], 'indeed': [4057], 'significantly': [4070], 'express': [4080], '(45': [4083], '(80': [4087], 'kDa).': [4088, 4132], 'When': [4089], 'large': [4097], '(C5aR-YFP)': [4098], 'small': [4100], '(C5aR)': [4101], 'products': [4108], '100,': [4110], '140,': [4111], '3B).': [4116], 'correspond': [4118], 'homodimer': [4121, 4130], '(100': [4122], 'kDa),': [4123, 4127], 'C5aR/C5aR-YFP': [4124], 'heterodimer': [4125], '(140': [4126], 'molecule,': [4139], 'additional': [4143, 4381], 'cysteines,': [4144, 4463], 'fused': [4146], 'reaction,': [4158], 'evidenced': [4160], 'binomial': [4164], 'distribution': [4165], 'species.': [4171], 'surprising': [4175], 'do': [4181], 'participate': [4183], 'cross-linking,': [4185], 'they': [4191], 'surface-accessible': [4194], '(40Wachter': [4195], 'R.M.': [4196], 'Elsliger': [4197], 'M.A.': [4198], 'Kallio': [4199], 'Hanson': [4201], 'G.T.': [4202], 'Remington': [4203], 'Structure.': [4205], '6:': [4207], '1267-1277Abstract': [4208], '(356)': [4216], 'it': [4220, 4233], 'important': [4222], 'dismiss': [4224], 'mediator': [4228], 'interaction,': [4231], 'dimerize': [4241], '(41Zacharias': [4245], 'Violin': [4247], 'J.D.': [4248], 'Newton': [4249], 'A.C.': [4250], 'Tsien': [4251], 'R.Y.': [4252], '296:': [4255], '913-916Crossref': [4256], '(1749)': [4259], 'Importantly,': [4262], 'claim': [4268], 'membrane-associated': [4280], 'protein.': [4290], 'Endogenous': [4293], 'Molecules': [4295], 'Human': [4297], 'Polymorphonuclear': [4298, 4319], 'Leukocytes—To': [4299], 'status': [4303], 'endogenously': [4305], 'molecules,': [4308], 'neutrophils.': [4318], 'leukocyte': [4320], 'urea;': [4329], 'EDTA.': [4350], 'treatment,': [4357], 'reveals': [4363], 'one': [4364], 'distinct': [4365], 'band': [4366], 'resulted': [4378], 'migrating': [4383], '95': [4385], '4A).': [4388], 'extent': [4398, 4425], 'trapping,': [4401, 4495], 'nm': [4407], 'monitored.': [4415], 'Ligand': [4416], 'no': [4421], '4B).': [4429], 'strongly': [4432], 'their': [4441], 'environment': [4443], 'altered': [4449], 'activation.': [4452], 'Mutation': [4453], 'Cysteine': [4455], '144': [4456], 'Abrogates': [4457], 'Trapping—': [4459], 'nine': [4462], '3-extracellular': [4471], 'bridge': [4475], 'among': [4477], 'identify': [4485], 'our': [4496], 'sequentially': [4500], 'mutate': [4501], 'remaining': [4503], 'seven': [4504], 'monitor': [4510]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1967562709', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 8}, {'year': 2012, 'cited_by_count': 6}], 'updated_date': '2024-12-13T18:24:11.714709', 'created_date': '2016-06-24'}