Title: Isolation and Characterization of the Cyanogen Bromide Peptides of the Collagen 2α Chain from a Transplantable Rat Chondrosarcoma
Abstract: The rat collagen 2α chain was isolated from a transplantable Swarm chondrosarcoma following limited pepsin proteolysis. The chromatographically purified 2α chain when cleaved with cyanogen bromide yields nine peptides which have been isolated and characterized with respect to their molecular weight and their amino acid composition. Eight of these peptides can be rapidly separated by gelpermeation high-performance liquid chromatography and retrieved for further purification by ion-exchange chromatography. The nine peptides are recovered in equimolar amounts and together account for a total of 1,014 amino acid residues. The features of the isolated cyanogen bromide peptides of the 2α chain clearly differentiate it from other known collagen polypeptides. The possible homologies between the 2α chain and other collagen a chains are noted. Comparison of the cyanogen bromide peptides indicates a close relationship of the 2α to the al (V) chain.
Publication Year: 1983
Publication Date: 1983-11-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 6
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