Title: Microdetermination of Hyoscyamine and Scopolamine in Mixtures
Abstract: Following the acid-dye technique, hyoscyamine (or atropine) could be selectively determined in the presence of scopolamine, using bromcresol purple at pH 6.6. Total alkaloids were determined using bromthymol blue at pH 5.6. By referring to calibration curves of the two alkaloids with the appropriate dye, the concentration of each alkaloid in the mixture could be computed. The suggested procedure was adopted to analyze the two alkaloids in synthetic mixtures. As low as 0.05 mg. of each alkaloid could be estimated, with average percentage recoveries of 98.6 and 101.5 for hyoscyamine and scopolamine, respectively. Tincture of belladonna was assayed following a modification of the suggested method. The results obtained were comparable with those obtained following the BP method.
Publication Year: 1973
Publication Date: 1973-08-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 13
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