Title: Subcellular sorting of isoactins: Selective association of γ actin with skeletal muscle mitochondria
Abstract: We describe two subpopulations of actin antibodies isolated by affinity chromatography from a polyclonal antibody to chicken gizzard actin. One subpopulation recognizes γ actins from smooth muscle and nonmuscle cells, but does not recognize α actin from skeletal muscle. The other subpopulation recognizes determinants that are common to a actin from skeletal muscle and the two γ actin isotypes. Neither antibody recognizes cytoplasmic β actin. Both antibodies recognize only actins or molecules with determinants that are also present in actins. By immunofluorescence we found that the anti-γ actin colocalizes with mitochondria in fibers of mouse diaphragm, and that it does not bind detectably to the I bands of sarcomeres. The antibody that recognizes both α and γ actins stains I bands intensely, as expected. We interpret these observations as preliminary evidence for selective association of γ actin with skeletal muscle mitochondria and, more broadly, as evidence for subcellular sorting of isoactins.
Publication Year: 1983
Publication Date: 1983-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 104
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