Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1965982125', 'doi': 'https://doi.org/10.1002/bip.20481', 'title': 'The effect of the osmolyte trimethylamine N‐oxide on the stability of the prion protein at low pH', 'display_name': 'The effect of the osmolyte trimethylamine N‐oxide on the stability of the prion protein at low pH', 'publication_year': 2006, 'publication_date': '2006-02-17', 'ids': {'openalex': 'https://openalex.org/W1965982125', 'doi': 'https://doi.org/10.1002/bip.20481', 'mag': '1965982125', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/16489585'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/bip.20481', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S56808608', 'display_name': 'Biopolymers', 'issn_l': '0006-3525', 'issn': ['0006-3525', '1097-0282'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5012544643', 'display_name': 'Vincenzo Granata', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I71267560', 'display_name': 'University of Naples Federico II', 'ror': 'https://ror.org/05290cv24', 'country_code': 'IT', 'type': 'education', 'lineage': ['https://openalex.org/I71267560']}, {'id': 'https://openalex.org/I4210115176', 'display_name': 'National Interuniversity Consortium for the Physical Sciences of Matter', 'ror': 'https://ror.org/026vvkz19', 'country_code': 'IT', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I4210115176']}], 'countries': ['IT'], 'is_corresponding': False, 'raw_author_name': 'Vincenzo Granata', 'raw_affiliation_strings': ['Dipartimento delle Scienze Biologiche-Sezione Biostrutture and CNISM, Università degli Studi di Napoli Federico II, Via Mezzocannone 16.I-80134 Napoli, Italy.'], 'affiliations': [{'raw_affiliation_string': 'Dipartimento delle Scienze Biologiche-Sezione Biostrutture and CNISM, Università degli Studi di Napoli Federico II, Via Mezzocannone 16.I-80134 Napoli, Italy.', 'institution_ids': ['https://openalex.org/I71267560', 'https://openalex.org/I4210115176']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5044762023', 'display_name': 'Pasquale Palladino', 'orcid': 'https://orcid.org/0000-0002-3869-5085'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Pasquale Palladino', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5027785999', 'display_name': 'Barbara Tizzano', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Barbara Tizzano', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5021059428', 'display_name': 'Alessandro Negro', 'orcid': 'https://orcid.org/0000-0003-3142-7632'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Alessandro Negro', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5075304102', 'display_name': 'Rita Berisio', 'orcid': 'https://orcid.org/0000-0001-5025-6483'}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Rita Berisio', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5054854944', 'display_name': 'Adriana Zagari', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'Adriana Zagari', 'raw_affiliation_strings': [], 'affiliations': []}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 3760, 'currency': 'USD', 'value_usd': 3760, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.705, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 27, 'citation_normalized_percentile': {'value': 0.777506, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 89, 'max': 90}, 'biblio': {'volume': '82', 'issue': '3', 'first_page': '234', 'last_page': '240'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11335', 'display_name': 'Prion Diseases: Causes and Molecular Basis', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11335', 'display_name': 'Prion Diseases: Causes and Molecular Basis', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11162', 'display_name': 'Macromolecular Crystallography Techniques', 'score': 0.9584, 'subfield': {'id': 'https://openalex.org/subfields/2505', 'display_name': 'Materials Chemistry'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T12441', 'display_name': 'Molecular Mechanisms of Heme Biosynthesis and Related Disorders', 'score': 0.954, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/osmolyte', 'display_name': 'Osmolyte', 'score': 0.9460726}, {'id': 'https://openalex.org/keywords/trimethylamine-n-oxide', 'display_name': 'Trimethylamine N-oxide', 'score': 0.7666451}, {'id': 'https://openalex.org/keywords/protein-stability', 'display_name': 'Protein Stability', 'score': 0.522565}], 'concepts': [{'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.95638025}, {'id': 'https://openalex.org/C127160389', 'wikidata': 'https://www.wikidata.org/wiki/Q415608', 'display_name': 'Osmolyte', 'level': 2, 'score': 0.9460726}, {'id': 'https://openalex.org/C2776149385', 'wikidata': 'https://www.wikidata.org/wiki/Q423953', 'display_name': 'Trimethylamine', 'level': 2, 'score': 0.8699519}, {'id': 'https://openalex.org/C2780924926', 'wikidata': 'https://www.wikidata.org/wiki/Q419802', 'display_name': 'Trimethylamine N-oxide', 'level': 3, 'score': 0.7666451}, {'id': 'https://openalex.org/C2988375501', 'wikidata': 'https://www.wikidata.org/wiki/Q847556', 'display_name': 'Protein stability', 'level': 2, 'score': 0.61308587}, {'id': 'https://openalex.org/C3019804061', 'wikidata': 'https://www.wikidata.org/wiki/Q14881255', 'display_name': 'Prion protein', 'level': 3, 'score': 0.60661066}, {'id': 'https://openalex.org/C2779851234', 'wikidata': 'https://www.wikidata.org/wiki/Q50690', 'display_name': 'Oxide', 'level': 2, 'score': 0.47532943}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.357216}, {'id': 'https://openalex.org/C178790620', 'wikidata': 'https://www.wikidata.org/wiki/Q11351', 'display_name': 'Organic chemistry', 'level': 1, 'score': 0.22448966}, {'id': 'https://openalex.org/C126322002', 'wikidata': 'https://www.wikidata.org/wiki/Q11180', 'display_name': 'Internal medicine', 'level': 1, 'score': 0.049114108}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C2779134260', 'wikidata': 'https://www.wikidata.org/wiki/Q12136', 'display_name': 'Disease', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D008744', 'descriptor_name': 'Methylamines', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': True}, {'descriptor_ui': 'D011328', 'descriptor_name': 'Prions', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002942', 'descriptor_name': 'Circular Dichroism', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004305', 'descriptor_name': 'Dose-Response Relationship, Drug', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006863', 'descriptor_name': 'Hydrogen-Ion Concentration', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008744', 'descriptor_name': 'Methylamines', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008744', 'descriptor_name': 'Methylamines', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D008956', 'descriptor_name': 'Models, Chemical', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018621', 'descriptor_name': 'PrPC Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D018621', 'descriptor_name': 'PrPC Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018620', 'descriptor_name': 'PrPSc Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D018620', 'descriptor_name': 'PrPSc Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011328', 'descriptor_name': 'Prions', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011328', 'descriptor_name': 'Prions', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011489', 'descriptor_name': 'Protein Denaturation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011489', 'descriptor_name': 'Protein Denaturation', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D017433', 'descriptor_name': 'Protein Structure, Secondary', 'qualifier_ui': 'Q000187', 'qualifier_name': 'drug effects', 'is_major_topic': False}, {'descriptor_ui': 'D017433', 'descriptor_name': 'Protein Structure, Secondary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012756', 'descriptor_name': 'Sheep', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013816', 'descriptor_name': 'Thermodynamics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1002/bip.20481', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S56808608', 'display_name': 'Biopolymers', 'issn_l': '0006-3525', 'issn': ['0006-3525', '1097-0282'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/16489585', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 31, 'referenced_works': ['https://openalex.org/W1492547275', 'https://openalex.org/W1576911364', 'https://openalex.org/W1594470823', 'https://openalex.org/W1704732331', 'https://openalex.org/W1974443289', 'https://openalex.org/W1976179026', 'https://openalex.org/W1980237498', 'https://openalex.org/W1982534182', 'https://openalex.org/W1985015433', 'https://openalex.org/W1989081602', 'https://openalex.org/W1995455102', 'https://openalex.org/W1998853944', 'https://openalex.org/W1999301383', 'https://openalex.org/W2005693512', 'https://openalex.org/W2012561030', 'https://openalex.org/W2017395920', 'https://openalex.org/W2022654057', 'https://openalex.org/W2023694825', 'https://openalex.org/W2028231353', 'https://openalex.org/W2060002111', 'https://openalex.org/W2069141366', 'https://openalex.org/W2076360029', 'https://openalex.org/W2078809854', 'https://openalex.org/W2080194107', 'https://openalex.org/W2080528351', 'https://openalex.org/W2082943498', 'https://openalex.org/W2096510701', 'https://openalex.org/W2141438296', 'https://openalex.org/W2148130200', 'https://openalex.org/W2167517570', 'https://openalex.org/W2324660492'], 'related_works': ['https://openalex.org/W4205565376', 'https://openalex.org/W3092515766', 'https://openalex.org/W2966808768', 'https://openalex.org/W2965139165', 'https://openalex.org/W2559400101', 'https://openalex.org/W2533606635', 'https://openalex.org/W2461670959', 'https://openalex.org/W2024610228', 'https://openalex.org/W1983363774', 'https://openalex.org/W1965982125'], 'abstract_inverted_index': {'Abstract': [0], 'A': [1], 'study': [2], 'of': [3, 6, 12, 16, 50, 95, 99, 128, 131, 142, 146, 187], 'the': [4, 10, 17, 48, 93, 96, 105, 124, 129, 143, 179, 188, 192], 'effect': [5, 130, 145], 'trimethylamine': [7, 51, 65, 100, 132], 'N‐oxide': [8, 66, 101, 133], 'on': [9, 134, 149], 'stability': [11, 72, 136], 'two': [13], 'recombinant': [14], 'forms': [15], 'prion': [18], 'protein': [19, 106], 'PrP,': [20], 'an': [21, 139, 170], 'ovine': [22], 'full‐length': [23], 'and': [24, 35, 46, 137], 'a': [25, 81, 185], 'human': [26], 'truncated': [27], 'form,': [28], 'is': [29, 54, 64, 90], 'here': [30], 'reported.': [31], 'Both': [32], 'thermal': [33, 71], 'denaturation': [34, 36], 'at': [37, 42, 73, 84, 151, 196], 'room': [38, 85], 'temperature': [39], 'were': [40], 'analyzed': [41], 'pH': [43, 75], 'values': [44], 'above': [45], 'below': [47], 'pKa': [49], 'N‐oxide,': [52], 'which': [53, 115], 'close': [55], 'to': [56, 68, 92, 102, 111, 178], '4.7.': [57], 'Surprisingly,': [58], 'results': [59, 122], 'showed': [60], 'that': [61], 'not': [62], 'only': [63], 'able': [67], 'decrease': [69], 'PrP': [70, 119, 135, 150], 'low': [74, 152], 'but': [76], 'it': [77], 'also': [78], 'acts': [79], 'as': [80, 108, 110, 169], 'strong': [82], 'denaturant': [83], 'temperature.': [86], 'Likely,': [87], 'this': [88, 147], 'destabilization': [89], 'due': [91], 'capability': [94], 'cationic': [97], 'form': [98], 'interact': [103], 'with': [104], 'backbone': [107], 'well': [109], 'weaken': [112], 'electrostatic': [113], 'interactions': [114], 'are': [116], 'important': [117], 'for': [118], 'fold.': [120], 'These': [121], 'constitute': [123], 'first': [125], 'experimental': [126], 'measurement': [127], 'provide': [138], 'unambiguous': [140], 'proof': [141], 'destabilizing': [144], 'osmolyte': [148], 'pH.': [153], '©': [154], '2006': [155, 162], 'Wiley': [156], 'Periodicals,': [157], 'Inc.': [158], 'Biopolymers': [159, 193], '82:': [160], '234–240,': [161], 'This': [163], 'article': [164], 'was': [165], 'originally': [166], 'published': [167], 'online': [168], 'accepted': [171], 'preprint.': [172], 'The': [173], '“Published': [174], 'Online”': [175], 'date': [176], 'corresponds': [177], 'preprint': [180, 189], 'version.': [181], 'You': [182], 'can': [183], 'request': [184], 'copy': [186], 'by': [190], 'emailing': [191], 'editorial': [194], 'office': [195], '[email protected]': [197]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1965982125', 'counts_by_year': [{'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-09-19T21:09:53.353824', 'created_date': '2016-06-24'}