Title: Localization of biotinylated monoclonal antibody in nude mice bearing subcutaneous and intraperitoneal human tumour xenografts
Abstract: International Journal of CancerVolume 41, Issue S3 p. 30-33 Article Localization of biotinylated monoclonal antibody in nude mice bearing subcutaneous and intraperitoneal human tumour xenografts S. Pervez, S. Pervez Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UK Imperial Cancer Research Fund Oncology Group, Department of Clinical Oncology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorG. Paganelli, G. Paganelli Servizio Medicina Nucleare, Ospedale “M. Bufalini” 47023 Cesena (FO), ItalySearch for more papers by this authorA. A. Epenetos, A. A. Epenetos Imperial Cancer Research Fund Oncology Group, Department of Clinical Oncology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorW. J. Mooi, W. J. Mooi Department of Pathology, The Netherlands Cancer Institute, Amsterdam, The NetherlandsSearch for more papers by this authorD. J. Evans, D. J. Evans Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorT. Krausz, Corresponding Author T. Krausz Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKDepartment of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this author S. Pervez, S. Pervez Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UK Imperial Cancer Research Fund Oncology Group, Department of Clinical Oncology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorG. Paganelli, G. Paganelli Servizio Medicina Nucleare, Ospedale “M. Bufalini” 47023 Cesena (FO), ItalySearch for more papers by this authorA. A. Epenetos, A. A. Epenetos Imperial Cancer Research Fund Oncology Group, Department of Clinical Oncology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorW. J. Mooi, W. J. Mooi Department of Pathology, The Netherlands Cancer Institute, Amsterdam, The NetherlandsSearch for more papers by this authorD. J. Evans, D. J. Evans Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this authorT. Krausz, Corresponding Author T. Krausz Department of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKDepartment of Histopathology, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 OHS, UKSearch for more papers by this author First published: 1988 https://doi.org/10.1002/ijc.2910410807Citations: 6AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract To demonstrate the precise distribution and binding of in vivo injected monoclonal antibodies on histological tumour sections, we have biotinylated our primary antibody AUAI. Biotinylated antibody was injected into nude mice bearing simultaneous subcutaneous and intraperitoncal xenografts of the human tumour LoVo. Twenty-four hours after injection, the animals were killed, tumours and control organs were removed. Antibody was demonstrated on frozen sections by incubating sections with avidin biotin peroxidase complex. We compared the in vivo penetration and binding of this antibody on intraperitoneal and subcutaneous xenografts. The antibody penetration was mainly restricted to a thin layer of tumour cells adjacent to the vascularized stroma in large solid subcutaneous and intraperitoneal tumours, whereas in very small intraperitoneal tumours, antibody penetration was complete. These findings were similar to our autoradiographic results. This study demonstrates that employing the biotinylated antibodies for in vivo localization studies provides superior resolution of antibody binding for morphological assessment compared to autoradiography. Localization of a biotin label is more precise and will permit ultra-structural studies. References Andrew, S. M., Pimm, M. V., Perkins, A. C., and Baldwin, R. W., Comparative imagine and biodistribution studies with an anti-CEA monoclonal antibody and its F(ab')2 and Fab fragments in mice with colon carcinoma xenografts. Europ. J. nucl. Med., 12, 168–175 (1986). 10.1007/BF00256915 CASPubMedWeb of Science®Google Scholar Arklie, J., Studies of the human epithelial cell surface using monoclonal antibodies, Ph.D. Thesis, University of Oxford (1981). Google Scholar Baldwin, R. W., and Pimm, M. V., Antitumour monoclonal antibodies for radioimmunotargetting of tumours and drug targeting. Cancer Metastasis Rev., 2, 89–106 (1983). 10.1007/BF00046907 CASPubMedWeb of Science®Google Scholar Bale, W. R., Contreas, A., Grady, E. D., Factors influencing localisation of labelled antibodies in tumours. Cancer Res., 40, 2965–2972 (1980). CASPubMedWeb of Science®Google Scholar Barry, D. M., Marvin, B. C., Romaine, E. S., Morton, D. L., Benedict, W. F., Edward, L. K., Spolter, L., Stephen, L. G., Chia, C., and Martin, W. B., Imaging of human tumour xenografts in nude mice with radiolabelled monoclonal antlibodies. Limitations of specificity due to non-specific uptake of antibody. Cancer, 54, 1318–1327 (1984). 10.1002/1097-0142(19841001)54:7<1318::AID-CNCR2820540715>3.0.CO;2-J PubMedWeb of Science®Google Scholar Drewinko, B., Romsdahl, M. M., Yang, L. Y., Ahearn, M. J., and Trijillo, J. M. E., Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res., 36, 467–475 (1976). CASPubMedWeb of Science®Google Scholar Fand, I., Sharkey, R. M., Primus, F. J., Cohen, S. A., and Goldemberg, D. M., Relationship of radioantibody localisation and cell viability in a xenografted human cancer model as measured by whole-body autoradiography. Cancer Res., 47, 2177–2183 (1987). CASPubMedWeb of Science®Google Scholar Jasani, B., Andrew, S. M., Schmid, K. W., Thomas, N. D., and Schmidhammer, H. A., Haem-specific reagent system for non-deleterious inhibition of endogenous peroxidase activity (EPA): application to immunoperoxidase staining of delicate antigens in EPA-rich preparations. J. Histochem. Cytochem. 35, 1031 (1987). Web of Science®Google Scholar Jones, P. L., Gallaher, B. M., and Sands, H., Autoradiographic analysis of monoclonal antibody distribution in human colon and breast tumour xenografts. Cancer Immunol. Imnmnother., 22, 139–143 (1986). 10.1007/BF00199128 CASPubMedWeb of Science®Google Scholar Lewis, J. C. M., Boxer, G. M., Searle, F., and Bagshawe, K. D., The comparative distribution of monoclonal antibodies to CEA in colorectal xenografts. Tumour Biol., 5, 255–261 (1984). CASPubMedWeb of Science®Google Scholar Marc, E. K., Michael, I., Lawrence, C. M., Kenneth, A. F., Robert, K. O., and Michael, G. H., Guinea pig line 10 hepatocarcinoma model for monoclonal antibody serotherapy: in vivo localisation of a monoclonal antibody in normal and malignant tissues. J. Immunol., 130, 1451–1457 (1983). PubMedWeb of Science®Google Scholar Mohakis, V., Ormerod, M. G., Westwood, J. H., Imrie, S., and Neville, A. M., The site of binding of anti-CEA antibodies to tumour in vivo: an immunocytochemical and autoradiographic approach. Brit. J. Cancer, 46, 18–21 (1982). 10.1038/bjc.1982.159 Web of Science®Google Scholar Paganelli, G., Riva, P., Deleide, G., Cuvio, A., Chiolerio, F., Scassellati, G. A., Malcovati, M., and Siccardi, A. G., In vivo labelling of biotinylated monoclonal antibodies by radioactive avidin, a strategy to increase tumour radiolocalization. Int. J. Cancer, Suppl. 2, 121–125 (1988). 10.1002/ijc.2910410727 CASPubMedGoogle Scholar Pervez, S., Epenetos, A. A., Mooi, W. J., Evans, D. J., Rowlinson, G., Dhokia, B., and Krausz, T., Localization of monoclonal antibody AUA1 and its F(ab')3 fragments in human tumour xenografts, An autoradiographic and immunohistochemical study. Int. J. Cancer, Suppl, 3, 23–29 (1988). 10.1002/ijc.2910410806 CASPubMedGoogle Scholar Reynals, F. D., Studies on the localisation of dyes and foreign proteins in normal and malignant tissues. Amer. J. Cancer, 35, 98–107 (1939). Google Scholar Rowlinson, G., Snook, D., Busza, A., and Epenetos, A. A., Antibody-guided localisation of intraperitoneal tumours following intraperitonel or intravenous antibody administration. Cancer Rev., 47, 6528–6531 (1987). CASPubMedWeb of Science®Google Scholar Stragand, J. J., Bergerat, J. P., White, R. A., Hokanson, J., and Drewinko, B., Biological and cell kinetic properties of a human colonic adenocarcinoma LoVo grown in athymic mice. Cancer Res., 40, 2846–2852 (1980). CASPubMedWeb of Science®Google Scholar Wood, G. S., and Warnke, R., Suppression of endogenous avidinbinding activity in tissues and its relevance to biotin-avidin detection systems. J. Histochem. Cytochem., 29(10), 1196–1204 (1981). 10.1177/29.10.7028859 CASPubMedWeb of Science®Google Scholar Citing Literature Volume41, IssueS3Supplement: Journal International du Cancer1988Pages 30-33 ReferencesRelatedInformation
Publication Year: 1988
Publication Date: 1988-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 11
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