Title: Immunoglobulin Expression in Human Lymphoblastoid Cell Lines with Early B Cell Features
Abstract: Immunoglobulin expression was studied by direct immunofluorescence and by biosynthesis experiments in two human cell lines Raji and T 5.1. The basic phenotype of these cells was close to that of pre‐B calls: large cell with intracytoplasmic IgM with a predominance of μ chains over light chains and no detectable surface immunoglobulins. The apparent molecular weight of heavy and light chains was abnormally large. Immunoglobulins were secreted at a low rate as pentameric IgM in the T 5.1 line and as subunits and free light chains in the Raji line. Spontaneous variations of this phenotype were observed: the cultured edit acquired surface μ and λ chains, then additionally δ chains while they progressively lost delectable cytoplasmic μ chains, thus leading to a mature B cell phenotype, Subsequently, the cells had no detectable surface and cytoplasmic immunoglobulins and then they displayed a pre‐B cell phenotype again. Attempts to induce further maturation using various potential inducers were unsuccessful
Publication Year: 1981
Publication Date: 1981-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 17
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