Title: Key elements of a highly efficient intracytoplasmic sperm injection technique: Ca2+ fluxes and oocyte cytoplasmic dislocation
Abstract: To analyze the mechanism by which modifications of the intracytoplasmic sperm injection (ICSI) technique influence success rates. Prospective clinical study supplemented with an experimental analysis of Ca2 + fluxes provoked by the injection procedure. Private hospital and public research center. Patients treated by IVF and ICSI. Intracytoplasmic sperm injection. Fertilization and pregnancy rates and intracellular free Ca2 + concentration. The inclusion of vigorous aspiration of oocyte cytoplasm improved outcomes of ICSI. In a series of 100 consecutive cases treated with this technique, the fertilization and pregnancy rates were 87% of total metaphase II oocytes injected and 52% of total treatment cycles, respectively. Enhanced Ca2 + influx into the injected oocytes and dislocation of the oocyte cytoplasm, including the development of a focus of persistent Ca2 + discharge around the injected sperm head, were the main characteristics of this highly successful technique. Vigorous aspiration of oocyte cytoplasm may facilitate fertilization after ICSI by increasing the oocyte Ca2 + load at the time of injection, by establishing a more intimate contact of the injected sperm head with oocyte intracellular Ca2 + stores, or by a conjunction of these mechanisms.
Publication Year: 1995
Publication Date: 1995-10-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 117
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