Title: Studies on Metabolic Pathway of NAD in Yeast
Abstract: Electrophoretically homogeneous preparation of yeast 5′~nucleotidase, one of NAD metabolizing system, was obtained from yeast autolysate by ammonium sulfate fractionation, chromatography, gel filtration and zone electrophoresis. The preparation had strong NAD-splitting activity, namely nucleotide pyrophosphatase activity. Throughout purification steps, the ratio of the two activities were kept constant and they could not be separated even by treatments with EDTA, urea, thioglycol and alkaline buffer. These seem to suggest that both activities of 5′-nucleotidase and nucleotide pyrophosphatase localize on a single protein.
Publication Year: 1967
Publication Date: 1967-08-01
Language: en
Type: article
Indexed In: ['crossref']
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