Title: Cold‐induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species
Abstract: The FASEB JournalVolume 13, Issue 1 p. 155-168 Research CommunicationFree to Read Cold-induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species URSULA RAUEN, Corresponding Author URSULA RAUEN n/[email protected] Institut für Physiologische Chemie, Universitätsklinikum, D-45122 Essen, GermanyCorrespondence: Institut für Physiologische Chemie, Universitätsklinikum, Hufelandstr. 55, D-45122 Essen, Germany.Search for more papers by this authorBERNHARD POLZAR, BERNHARD POLZAR Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHARALD STEPHAN, HARALD STEPHAN Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHANS GEORG MANNHERZ, HANS GEORG MANNHERZ Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHERBERT DE GROOT, HERBERT DE GROOT Institut für Physiologische Chemie, Universitätsklinikum, D-45122 Essen, GermanySearch for more papers by this author URSULA RAUEN, Corresponding Author URSULA RAUEN n/[email protected] Institut für Physiologische Chemie, Universitätsklinikum, D-45122 Essen, GermanyCorrespondence: Institut für Physiologische Chemie, Universitätsklinikum, Hufelandstr. 55, D-45122 Essen, Germany.Search for more papers by this authorBERNHARD POLZAR, BERNHARD POLZAR Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHARALD STEPHAN, HARALD STEPHAN Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHANS GEORG MANNHERZ, HANS GEORG MANNHERZ Abteilung für Anatomie und Embryologie, Ruhr-Universität, D-44780 Bochum, GermanySearch for more papers by this authorHERBERT DE GROOT, HERBERT DE GROOT Institut für Physiologische Chemie, Universitätsklinikum, D-45122 Essen, GermanySearch for more papers by this author First published: 01 January 1999 https://doi.org/10.1096/fasebj.13.1.155Citations: 56Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat ABSTRACT When cultured hepatocytes were incubated in cell culture medium at 4°C for up to 30 h and then returned to 37°C, blebbing of the plasma membrane, cell detachment, chromatin condensation and margination, enhanced nuclear stainability with Hoechst 33342, ruffling of the nuclear membrane, and DNA fragmentation occurred. Similar to hepatocytes, cultured liver endothelial cells exhibited blebbing, chromatin condensation and margination, marked nuclear condensation, and increased stainability with Hoechst 33342 when exposed to hypothermia/rewarming. In both cell types, the occurrence and extent of these alterations were dependent on the duration of the cold incubation period. This cold-induced apoptosis was inhibited by hypoxia, by an array of free radical scavengers/antioxidants, and by iron chelators. However, the extent of the protection by the different antioxidants was different in the two cell types: iron chelators provided complete protection in liver endothelial cells but only partial protection in hepatocytes, whereas lipophilic antioxidants such as α-tocopherol provided complete protection in both cell types. During cold incubation, and especially during rewarming, lipid peroxidation occurred. These results suggest that the formation of reactive oxygen species (ROS) is a key mediator of cold-induced apoptosis, with ROS formation being completely iron-mediated in liver endothelial cells and partially iron-mediated in hepatocytes.—Rauen, U., Polzar, B., Stephan, H., Mannherz, H. G., de Groot, H. Cold-induced apoptosis in cultured hepatocytes and liver endothelial cells: mediation by reactive oxygen species. FASEB J. 13, 155–168 (1999) Citing Literature Volume13, Issue1January 1999Pages 155-168 RelatedInformation
Publication Year: 1999
Publication Date: 1999-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 319
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