Title: Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity
Abstract: Article1 December 1983free access Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity P. Zambryski P. Zambryski Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author H. Joos H. Joos Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author C. Genetello C. Genetello Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author J. Leemans J. Leemans Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode, Belgium To whom reprint requests should be sent. Search for more papers by this author M. Van Montagu M. Van Montagu Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode, Belgium Present address: Centro de investigaciones sobre fijacion de nitrogeno, Ap. postal 565-A, Cuernavaca, Morelos, Mexico. Search for more papers by this author J. Schell J. Schell Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG Present address: Centro de investigaciones sobre fijacion de nitrogeno, Ap. postal 565-A, Cuernavaca, Morelos, Mexico. Search for more papers by this author P. Zambryski P. Zambryski Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author H. Joos H. Joos Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author C. Genetello C. Genetello Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Search for more papers by this author J. Leemans J. Leemans Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode, Belgium To whom reprint requests should be sent. Search for more papers by this author M. Van Montagu M. Van Montagu Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode, Belgium Present address: Centro de investigaciones sobre fijacion de nitrogeno, Ap. postal 565-A, Cuernavaca, Morelos, Mexico. Search for more papers by this author J. Schell J. Schell Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG Present address: Centro de investigaciones sobre fijacion de nitrogeno, Ap. postal 565-A, Cuernavaca, Morelos, Mexico. Search for more papers by this author Author Information P. Zambryski1, H. Joos1, C. Genetello1, J. Leemans2, M. Van Montagu1,2 and J. Schell1,3 1Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium 2Laboratorium voor Genetische Virologie, Vrije Universiteit Brussel, B-1640 St.-Genesius-Rode, Belgium 3Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, FRG The EMBO Journal (1983)2:2143-2150https://doi.org/10.1002/j.1460-2075.1983.tb01715.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info A Ti plasmid mutant was constructed in which all the on-cogenic functions of the T-DNA have been deleted and replaced by pBR322. This Ti plasmid, pGV3850, still mediates efficient transfer and stabilization of its truncated T-DNA into infected plant cells. Moreover, integration and expression of this minimal T-DNA in plant cells does not interfere with normal plant cell differentiation. A DNA fragment cloned in a pBR vector can be inserted in the pGV3850 T-region upon a single recombination event through the pBR322 region of pGV3850 producing a co-integrate useful for the transformation of plant cells. Based upon these properties, pGV3850 is proposed as an extremely versatile vector for the introduction of any DNA of interest into plant cells. Previous ArticleNext Article Volume 2Issue 121 December 1983In this issue RelatedDetailsLoading ...