Title: The secD locus of E.coli codes for two membrane proteins required for protein export.
Abstract: Research Article1 October 1990free access The secD locus of E.coli codes for two membrane proteins required for protein export. C. Gardel C. Gardel Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author K. Johnson K. Johnson Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author A. Jacq A. Jacq Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author J. Beckwith J. Beckwith Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author C. Gardel C. Gardel Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author K. Johnson K. Johnson Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author A. Jacq A. Jacq Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author J. Beckwith J. Beckwith Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. Search for more papers by this author Author Information C. Gardel1, K. Johnson1, A. Jacq1 and J. Beckwith1 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115. The EMBO Journal (1990)9:3209-3216https://doi.org/10.1002/j.1460-2075.1990.tb07519.x Correction(s) for this article The secD locus of E. coli codes for two membrane proteins required for protein export.01 December 1990 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cold-sensitive mutations in the secD locus of Escherichia coli result in severe defects in protein export at the non-permissive temperature of 23 degrees C. DNA sequence of a cloned fragment that includes the secD locus reveals open reading frames for seven polypeptide chains. Both deletions and TnphoA insertions in this clone have been used in maxicell and complementation studies to define the secD locus and its products. The secD mutations fall into two complementation groups, defining genes we have named secD and secF. These two genes comprise an operon, the first case of two genes involved in the export process being co-transcribed. The DNA sequence of the two genes along with alkaline phosphatase fusion analysis indicates that they code for integral proteins of the cytoplasmic membrane. We suggest that these two proteins may form a complex in the membrane which acts at late steps in the export process. Previous ArticleNext Article Volume 9Issue 101 October 1990In this issue RelatedDetailsLoading ...