Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W187774752', 'doi': 'https://doi.org/10.1007/978-1-59745-209-0_1', 'title': 'A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag', 'display_name': 'A Generic Method for the Production of Recombinant Proteins in Escherichia coli Using a Dual Hexahistidine-Maltose-Binding Protein Affinity Tag', 'publication_year': 2007, 'publication_date': '2007-01-01', 'ids': {'openalex': 'https://openalex.org/W187774752', 'doi': 'https://doi.org/10.1007/978-1-59745-209-0_1', 'mag': '187774752', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/17272834'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/978-1-59745-209-0_1', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210172139', 'display_name': 'Methods in molecular biology', 'issn_l': '1064-3745', 'issn': ['1064-3745', '1940-6029'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'book series'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'book-chapter', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5051185590', 'display_name': 'Joseph E. Tropea', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210130649', 'display_name': 'Frederick National Laboratory for Cancer Research', 'ror': 'https://ror.org/03v6m3209', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210130649', 'https://openalex.org/I4210140884']}, {'id': 'https://openalex.org/I4210149717', 'display_name': 'Center for Cancer Research', 'ror': 'https://ror.org/05bjen692', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210140884', 'https://openalex.org/I4210149717']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Joseph E. Tropea', 'raw_affiliation_strings': ['Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick'], 'affiliations': [{'raw_affiliation_string': 'Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick', 'institution_ids': ['https://openalex.org/I4210130649', 'https://openalex.org/I4210149717']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5089863098', 'display_name': 'Scott Cherry', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210130649', 'display_name': 'Frederick National Laboratory for Cancer Research', 'ror': 'https://ror.org/03v6m3209', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210130649', 'https://openalex.org/I4210140884']}, {'id': 'https://openalex.org/I4210149717', 'display_name': 'Center for Cancer Research', 'ror': 'https://ror.org/05bjen692', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210140884', 'https://openalex.org/I4210149717']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Scott Cherry', 'raw_affiliation_strings': ['Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick'], 'affiliations': [{'raw_affiliation_string': 'Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick', 'institution_ids': ['https://openalex.org/I4210130649', 'https://openalex.org/I4210149717']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5080592240', 'display_name': 'Sreedevi Nallamsetty', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210130649', 'display_name': 'Frederick National Laboratory for Cancer Research', 'ror': 'https://ror.org/03v6m3209', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210130649', 'https://openalex.org/I4210140884']}, {'id': 'https://openalex.org/I4210149717', 'display_name': 'Center for Cancer Research', 'ror': 'https://ror.org/05bjen692', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210140884', 'https://openalex.org/I4210149717']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Sreedevi Nallamsetty', 'raw_affiliation_strings': ['Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick'], 'affiliations': [{'raw_affiliation_string': 'Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick', 'institution_ids': ['https://openalex.org/I4210130649', 'https://openalex.org/I4210149717']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5008132760', 'display_name': 'Christophe Bignon', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210146787', 'display_name': 'Architecture et Fonction des Macromolécules Biologiques', 'ror': 'https://ror.org/04jm8zw14', 'country_code': 'FR', 'type': 'facility', 'lineage': ['https://openalex.org/I1294671590', 'https://openalex.org/I21491767', 'https://openalex.org/I4210088668', 'https://openalex.org/I4210096427', 'https://openalex.org/I4210146787']}, {'id': 'https://openalex.org/I1294671590', 'display_name': 'Centre National de la Recherche Scientifique', 'ror': 'https://ror.org/02feahw73', 'country_code': 'FR', 'type': 'government', 'lineage': ['https://openalex.org/I1294671590']}], 'countries': ['FR'], 'is_corresponding': False, 'raw_author_name': 'Christophe Bignon', 'raw_affiliation_strings': ['Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, CNRS, Marseille, France', 'Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, Université de Provence, Marseille, France', 'Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, Université de la Méditerrané, Marseille, France'], 'affiliations': [{'raw_affiliation_string': 'Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, CNRS, Marseille, France', 'institution_ids': ['https://openalex.org/I4210146787', 'https://openalex.org/I1294671590']}, {'raw_affiliation_string': 'Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, Université de Provence, Marseille, France', 'institution_ids': ['https://openalex.org/I4210146787']}, {'raw_affiliation_string': 'Glycogenomics and Biomedical Structural Biology, Architecture et Fonction des Macromolécules Biologiques, Université de la Méditerrané, Marseille, France', 'institution_ids': ['https://openalex.org/I4210146787']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5072856130', 'display_name': 'David S. Waugh', 'orcid': 'https://orcid.org/0000-0003-0776-8488'}, 'institutions': [{'id': 'https://openalex.org/I4210130649', 'display_name': 'Frederick National Laboratory for Cancer Research', 'ror': 'https://ror.org/03v6m3209', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210130649', 'https://openalex.org/I4210140884']}, {'id': 'https://openalex.org/I4210149717', 'display_name': 'Center for Cancer Research', 'ror': 'https://ror.org/05bjen692', 'country_code': 'US', 'type': 'facility', 'lineage': ['https://openalex.org/I1299022934', 'https://openalex.org/I1299303238', 'https://openalex.org/I4210140884', 'https://openalex.org/I4210149717']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'David S. Waugh', 'raw_affiliation_strings': ['Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick'], 'affiliations': [{'raw_affiliation_string': 'Protein Engineering Section, Macromolecular Crystallography Laboratory, Center for Cancer Research, National Cancer Institute-Frederick, Frederick', 'institution_ids': ['https://openalex.org/I4210130649', 'https://openalex.org/I4210149717']}]}], 'institution_assertions': [], 'countries_distinct_count': 2, 'institutions_distinct_count': 4, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 3.734, 'has_fulltext': False, 'cited_by_count': 67, 'citation_normalized_percentile': {'value': 0.802122, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 95, 'max': 96}, 'biblio': {'volume': None, 'issue': None, 'first_page': '1', 'last_page': '19'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11124', 'display_name': 'Protein Aggregation and Biopharmaceutical Stability', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11124', 'display_name': 'Protein Aggregation and Biopharmaceutical Stability', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11016', 'display_name': 'Therapeutic Antibodies: Development, Engineering, and Applications', 'score': 0.9996, 'subfield': {'id': 'https://openalex.org/subfields/2741', 'display_name': 'Radiology, Nuclear Medicine and Imaging'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T11048', 'display_name': 'Ecology and Evolution of Viruses in Ecosystems', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/maltose-binding-protein', 'display_name': 'Maltose-binding protein', 'score': 0.94465435}, {'id': 'https://openalex.org/keywords/tobacco-etch-virus', 'display_name': 'Tobacco etch virus', 'score': 0.66574764}, {'id': 'https://openalex.org/keywords/flag-tag', 'display_name': 'FLAG-tag', 'score': 0.56700003}, {'id': 'https://openalex.org/keywords/protein-tag', 'display_name': 'Protein tag', 'score': 0.55491805}, {'id': 'https://openalex.org/keywords/protein-aggregation', 'display_name': 'Protein Aggregation', 'score': 0.531984}, {'id': 'https://openalex.org/keywords/recombinant-proteins', 'display_name': 'Recombinant Proteins', 'score': 0.504273}, {'id': 'https://openalex.org/keywords/myc-tag', 'display_name': 'Myc-tag', 'score': 0.5009382}, {'id': 'https://openalex.org/keywords/protein-purification', 'display_name': 'Protein purification', 'score': 0.47308165}], 'concepts': [{'id': 'https://openalex.org/C2780676925', 'wikidata': 'https://www.wikidata.org/wiki/Q24741276', 'display_name': 'Maltose-binding protein', 'level': 5, 'score': 0.94465435}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.71643156}, {'id': 'https://openalex.org/C179247698', 'wikidata': 'https://www.wikidata.org/wiki/Q382799', 'display_name': 'Affinity chromatography', 'level': 3, 'score': 0.69085205}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.6820466}, {'id': 'https://openalex.org/C2777833472', 'wikidata': 'https://www.wikidata.org/wiki/Q3560918', 'display_name': 'Tobacco etch virus', 'level': 5, 'score': 0.66574764}, {'id': 'https://openalex.org/C123894998', 'wikidata': 'https://www.wikidata.org/wiki/Q425158', 'display_name': 'Fusion protein', 'level': 4, 'score': 0.58263457}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.58132696}, {'id': 'https://openalex.org/C30417494', 'wikidata': 'https://www.wikidata.org/wiki/Q3382226', 'display_name': 'FLAG-tag', 'level': 5, 'score': 0.56700003}, {'id': 'https://openalex.org/C156904656', 'wikidata': 'https://www.wikidata.org/wiki/Q645590', 'display_name': 'Protein tag', 'level': 5, 'score': 0.55491805}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.53396875}, {'id': 'https://openalex.org/C136071829', 'wikidata': 'https://www.wikidata.org/wiki/Q6946829', 'display_name': 'Myc-tag', 'level': 5, 'score': 0.5009382}, {'id': 'https://openalex.org/C8985478', 'wikidata': 'https://www.wikidata.org/wiki/Q903485', 'display_name': 'Protein purification', 'level': 2, 'score': 0.47308165}, {'id': 'https://openalex.org/C2776714187', 'wikidata': 'https://www.wikidata.org/wiki/Q212410', 'display_name': 'Protease', 'level': 3, 'score': 0.42982668}, {'id': 'https://openalex.org/C43617362', 'wikidata': 'https://www.wikidata.org/wiki/Q170050', 'display_name': 'Chromatography', 'level': 1, 'score': 0.3823169}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.17627728}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.15302598}, {'id': 'https://openalex.org/C109110057', 'wikidata': 'https://www.wikidata.org/wiki/Q1495434', 'display_name': 'Plant virus', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C2522874641', 'wikidata': 'https://www.wikidata.org/wiki/Q808', 'display_name': 'Virus', 'level': 2, 'score': 0.0}, {'id': 'https://openalex.org/C2776625791', 'wikidata': 'https://www.wikidata.org/wiki/Q3400142', 'display_name': 'Potyvirus', 'level': 4, 'score': 0.0}, {'id': 'https://openalex.org/C159047783', 'wikidata': 'https://www.wikidata.org/wiki/Q7215', 'display_name': 'Virology', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D006639', 'descriptor_name': 'Histidine', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D009842', 'descriptor_name': 'Oligopeptides', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002846', 'descriptor_name': 'Chromatography, Affinity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D006639', 'descriptor_name': 'Histidine', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D006639', 'descriptor_name': 'Histidine', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D058619', 'descriptor_name': 'Maltose-Binding Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009842', 'descriptor_name': 'Oligopeptides', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D009842', 'descriptor_name': 'Oligopeptides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1007/978-1-59745-209-0_1', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4210172139', 'display_name': 'Methods in molecular biology', 'issn_l': '1064-3745', 'issn': ['1064-3745', '1940-6029'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310319900', 'host_organization_name': 'Springer Science+Business Media', 'host_organization_lineage': ['https://openalex.org/P4310319965', 'https://openalex.org/P4310319900'], 'host_organization_lineage_names': ['Springer Nature', 'Springer Science+Business Media'], 'type': 'book series'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/17272834', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.85, 'display_name': 'Good health and well-being', 'id': 'https://metadata.un.org/sdg/3'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 72, 'referenced_works': ['https://openalex.org/W1480821243', 'https://openalex.org/W1498439983', 'https://openalex.org/W1530482967', 'https://openalex.org/W1531234455', 'https://openalex.org/W1547455336', 'https://openalex.org/W1597533137', 'https://openalex.org/W1640152225', 'https://openalex.org/W1688901160', 'https://openalex.org/W1881326155', 'https://openalex.org/W1922584555', 'https://openalex.org/W1964194110', 'https://openalex.org/W1967214752', 'https://openalex.org/W1967798978', 'https://openalex.org/W1980242161', 'https://openalex.org/W1983104210', 'https://openalex.org/W1988836624', 'https://openalex.org/W1989032771', 'https://openalex.org/W1993689607', 'https://openalex.org/W1993926172', 'https://openalex.org/W2000064612', 'https://openalex.org/W2004114526', 'https://openalex.org/W2016336609', 'https://openalex.org/W2022252180', 'https://openalex.org/W2029842408', 'https://openalex.org/W2030860360', 'https://openalex.org/W2031603364', 'https://openalex.org/W2035444171', 'https://openalex.org/W2037356132', 'https://openalex.org/W2038251099', 'https://openalex.org/W2042840404', 'https://openalex.org/W2047188621', 'https://openalex.org/W2048753590', 'https://openalex.org/W2057145553', 'https://openalex.org/W2071804506', 'https://openalex.org/W2072737163', 'https://openalex.org/W2075120021', 'https://openalex.org/W2078080788', 'https://openalex.org/W2079967105', 'https://openalex.org/W2082707643', 'https://openalex.org/W2084597963', 'https://openalex.org/W2084641790', 'https://openalex.org/W2085272777', 'https://openalex.org/W2085345576', 'https://openalex.org/W2085791091', 'https://openalex.org/W2087456101', 'https://openalex.org/W2088850938', 'https://openalex.org/W2093109164', 'https://openalex.org/W2093540751', 'https://openalex.org/W2096071764', 'https://openalex.org/W2096575861', 'https://openalex.org/W2097152121', 'https://openalex.org/W2105778990', 'https://openalex.org/W2118485393', 'https://openalex.org/W2132017330', 'https://openalex.org/W2136285267', 'https://openalex.org/W2139305314', 'https://openalex.org/W2140139924', 'https://openalex.org/W2145559272', 'https://openalex.org/W2154098162', 'https://openalex.org/W2160898059', 'https://openalex.org/W2161933361', 'https://openalex.org/W2163076661', 'https://openalex.org/W2167038708', 'https://openalex.org/W2169580261', 'https://openalex.org/W2804007336', 'https://openalex.org/W30959876', 'https://openalex.org/W3140762575', 'https://openalex.org/W32169690', 'https://openalex.org/W4237070259', 'https://openalex.org/W4285719527', 'https://openalex.org/W71280932', 'https://openalex.org/W77934935'], 'related_works': ['https://openalex.org/W771021118', 'https://openalex.org/W30959876', 'https://openalex.org/W2937981232', 'https://openalex.org/W2758243789', 'https://openalex.org/W2620923907', 'https://openalex.org/W2339087750', 'https://openalex.org/W2188507493', 'https://openalex.org/W2114522205', 'https://openalex.org/W187774752', 'https://openalex.org/W1586297894'], 'abstract_inverted_index': {'A': [0], 'generic': [1], 'protocol': [2], 'that': [3, 110], 'utilizes': [4], 'a': [5, 92, 150], 'dual': [6], 'hexahistidine-maltose-binding': [7], 'protein': [8, 37, 46, 103, 148], '(His6-MBP)': [9], 'affinity': [10, 53, 152], 'tag': [11], 'has': [12], 'been': [13], 'developed': [14], 'for': [15], 'the': [16, 28, 32, 35, 39, 73, 76, 80, 83, 87, 100, 105, 113, 117, 125, 131, 143], 'production': [17], 'of': [18, 34, 86, 95, 128, 133], 'recombinant': [19], 'proteins': [20, 109], 'in': [21, 64, 104], 'Escherichia': [22], 'coli.': [23], 'The': [24, 44], 'MBP': [25], 'moiety': [26], 'improves': [27], 'yield': [29], 'and': [30, 61], 'enhances': [31], 'solubility': [33], 'passenger': [36, 77, 102], 'while': [38], 'His-tag': [40], 'facilitates': [41], 'its': [42], 'purification.': [43], 'fusion': [45], '(His6-MBP-passenger)': [47], 'is': [48, 142], 'purified': [49], 'by': [50, 91], 'immobilized': [51], 'metal': [52], 'chromatography': [54], '(IMAC)': [55], 'on': [56], 'nickel-nitrilotriacetic': [57], 'acid': [58], '(Ni-NTA)': [59], 'resin': [60, 115], 'then': [62], 'cleaved': [63], 'vitro': [65], 'with': [66, 149], 'His6-tobacco': [67], 'etch': [68], 'virus': [69], 'protease': [70], 'to': [71, 112, 145], 'separate': [72], 'His6-MBP': [74], 'from': [75], 'protein.': [78], 'In': [79], 'final': [81], 'step,': [82], 'unwanted': [84], 'byproducts': [85], 'digest': [88], 'are': [89], 'absorbed': [90], 'second': [93, 126], 'round': [94, 127], 'IMAC,': [96], 'leaving': [97], 'nothing': [98], 'but': [99], 'pure': [101], 'flow-through': [106], 'fraction.': [107], 'Endogenous': [108], 'bind': [111], 'Ni-NTA': [114], 'during': [116, 124], 'first': [118], 'IMAC': [119, 136], 'step': [120], 'also': [121], 'do': [122], 'so': [123], 'IMAC.': [129], 'Hence,': [130], 'application': [132], 'two': [134], 'successive': [135], 'steps,': [137], 'rather': [138], 'than': [139], 'just': [140], 'one,': [141], 'key': [144], 'obtaining': [146], 'crystallization-grade': [147], 'single': [151], 'technique.': [153]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W187774752', 'counts_by_year': [{'year': 2021, 'cited_by_count': 4}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 7}, {'year': 2014, 'cited_by_count': 1}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 6}], 'updated_date': '2024-09-14T03:16:33.962668', 'created_date': '2016-06-24'}