Abstract: A major advance in DNA methylation analysis was the development of a method for sodium bisulfite modification of DNA to convert unmethylated cytosines to uracil, leaving methylated cytosines unchanged. High performance liquid chromatography is a classical method to quantify global DNA methylation and is highly quantitative and reproducible, but this can also be measured by flow cytometry and immunohistochemistry. Gene-specific methylation analysis methods can be characterized as either candidate gene or genome-wide approaches. The former cam be divided into non-microarray and microarray analyses. A widely used possibility is called methylated DNA immunoprecipitation, in which DNA is immunoprecipitated using an anti-5-methylcytosine antibody, then hybridized to a microarray. Bisulfite-pyrosequencing relies on bisulfite conversion and PCR amplification; pyrosequencing is a primer extension method for the analysis of short to medium-length DNA sequences. Whole-genome bisulfite sequencing has been achieved on the Illumina Genome Analyzer platform.
Publication Year: 2016
Publication Date: 2016-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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