Title: DETECTION OF BACILLUS CEREUS CONTAINING VOITOXIN IN FOOD BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD
Abstract: Journal of Food SafetyVolume 31, Issue 3 p. 313-319 DETECTION OF BACILLUS CEREUS CONTAINING VOITOXIN IN FOOD BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION METHOD ZHIGUO LIU, ZHIGUO LIU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this authorGUOLIANG FANG, GUOLIANG FANG School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorQI LI, Corresponding Author QI LI School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China TEL: 86-27-83956736; FAX: 86-27-83956940; EMAIL: [email protected] for more papers by this authorGUOPING ZHOU, GUOPING ZHOU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorRUI LI, RUI LI School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorYUNJIE FU, YUNJIE FU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorHE LI, HE LI Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this authorSHEN QU, SHEN QU Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this author ZHIGUO LIU, ZHIGUO LIU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this authorGUOLIANG FANG, GUOLIANG FANG School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorQI LI, Corresponding Author QI LI School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. China TEL: 86-27-83956736; FAX: 86-27-83956940; EMAIL: [email protected] for more papers by this authorGUOPING ZHOU, GUOPING ZHOU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorRUI LI, RUI LI School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorYUNJIE FU, YUNJIE FU School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, P. R. ChinaSearch for more papers by this authorHE LI, HE LI Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this authorSHEN QU, SHEN QU Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. ChinaSearch for more papers by this author First published: 04 July 2011 https://doi.org/10.1111/j.1745-4565.2011.00302.xCitations: 7Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract ABSTRACT Bacillus cereus strains containing voitoxin cereulide are pathogenic bacteria. The cereulide is produced by B. cereus strains containing plasmid pCER270. To establish a specific loop-mediated isothermal amplification (LAMP) method detecting pathogenic B. cereus, LAMP primer sets were designed based on 16SrDNA and pCER270 plasmid cesA sequence. The LAMP reaction was carried out under optimal conditions with 2 mM of Mg2+ at 65C for 1 h. Specificity of LAMP primers were validated by assaying 19 B. cereus strains with and without pCER270 plasmid and 42 non-B. cereus species. Detection limits of the LAMP assay were 1 cfu/mL of B. cereus without pCER270 plasmid and 11 cfu/mL of pathogenic B. cereus with pCER270 plasmid. Furthermore, liquid milk samples were detected by the LAMP method. The results showed that LAMP method is a reliable method used for rapid detection of B. cereus and its pathogenic strains of milk and other food stuff. PRACTICAL APPLICATIONS Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method. In this study LAMP method was specific and sensitive for detection of B. cereus which grown in liquid medium or on solid medium, or from liquid milk samples as well. In addition, the pathogenic strains expressed voitoxin cereulide could be readily identified after amplification using specific LAMP primer sets. The whole procedure is simple and rapid, the reaction carried out in tens of minutes at a constant temperature. LAMP could be routinely used for B. cereus especially pathogenic strains in food. Citing Literature Volume31, Issue3August 2011Pages 313-319 RelatedInformation
Publication Year: 2011
Publication Date: 2011-07-04
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 10
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