Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1821564040', 'doi': 'https://doi.org/10.1271/bbb.63.1107', 'title': 'Cloning and Sequencing of the β-Fructofuranosidase gene from<i>Bacillus</i>sp. V230', 'display_name': 'Cloning and Sequencing of the β-Fructofuranosidase gene from<i>Bacillus</i>sp. V230', 'publication_year': 1999, 'publication_date': '1999-01-01', 'ids': {'openalex': 'https://openalex.org/W1821564040', 'doi': 'https://doi.org/10.1271/bbb.63.1107', 'mag': '1821564040', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/10427700'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1271/bbb.63.1107', 'pdf_url': 'https://academic.oup.com/bbb/article-pdf/63/6/1107/35019068/bbb1107.pdf', 'source': {'id': 'https://openalex.org/S94095188', 'display_name': 'Bioscience Biotechnology and Biochemistry', 'issn_l': '0916-8451', 'issn': ['0916-8451', '1347-6947'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://academic.oup.com/bbb/article-pdf/63/6/1107/35019068/bbb1107.pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5103372479', 'display_name': 'Masashi Kurimoto', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104993', 'display_name': 'Hayashibara (Japan)', 'ror': 'https://ror.org/01rvpy991', 'country_code': 'JP', 'type': 'company', 'lineage': ['https://openalex.org/I4210104993']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Masashi KURIMOTO', 'raw_affiliation_strings': ['Hayashibara Biochemical Laboratories, Inc.'], 'affiliations': [{'raw_affiliation_string': 'Hayashibara Biochemical Laboratories, Inc.', 'institution_ids': ['https://openalex.org/I4210104993']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5017161768', 'display_name': 'Keiji Tsusaki', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104993', 'display_name': 'Hayashibara (Japan)', 'ror': 'https://ror.org/01rvpy991', 'country_code': 'JP', 'type': 'company', 'lineage': ['https://openalex.org/I4210104993']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Keiji TSUSAKI', 'raw_affiliation_strings': ['Hayashibara Biochemical Laboratories, Inc.'], 'affiliations': [{'raw_affiliation_string': 'Hayashibara Biochemical Laboratories, Inc.', 'institution_ids': ['https://openalex.org/I4210104993']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5012570704', 'display_name': 'Michio Kubota', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104993', 'display_name': 'Hayashibara (Japan)', 'ror': 'https://ror.org/01rvpy991', 'country_code': 'JP', 'type': 'company', 'lineage': ['https://openalex.org/I4210104993']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Michio KUBOTA', 'raw_affiliation_strings': ['Hayashibara Biochemical Laboratories, Inc.'], 'affiliations': [{'raw_affiliation_string': 'Hayashibara Biochemical Laboratories, Inc.', 'institution_ids': ['https://openalex.org/I4210104993']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5113610048', 'display_name': 'Shigeharu Fukuda', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104993', 'display_name': 'Hayashibara (Japan)', 'ror': 'https://ror.org/01rvpy991', 'country_code': 'JP', 'type': 'company', 'lineage': ['https://openalex.org/I4210104993']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Shigeharu FUKUDA', 'raw_affiliation_strings': ['Hayashibara Biochemical Laboratories, Inc.'], 'affiliations': [{'raw_affiliation_string': 'Hayashibara Biochemical Laboratories, Inc.', 'institution_ids': ['https://openalex.org/I4210104993']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5069212057', 'display_name': 'Yoshio Tsujisaka', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210104993', 'display_name': 'Hayashibara (Japan)', 'ror': 'https://ror.org/01rvpy991', 'country_code': 'JP', 'type': 'company', 'lineage': ['https://openalex.org/I4210104993']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Yoshio TSUJISAKA', 'raw_affiliation_strings': ['Hayashibara Biochemical Laboratories, Inc.'], 'affiliations': [{'raw_affiliation_string': 'Hayashibara Biochemical Laboratories, Inc.', 'institution_ids': ['https://openalex.org/I4210104993']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 4705, 'currency': 'USD', 'value_usd': 4705, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.835, 'has_fulltext': False, 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.500487, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 70, 'max': 72}, 'biblio': {'volume': '63', 'issue': '6', 'first_page': '1107', 'last_page': '1111'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12489', 'display_name': 'Microbial Metabolites in Food Biotechnology', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2916', 'display_name': 'Nutrition and Dietetics'}, 'field': {'id': 'https://openalex.org/fields/29', 'display_name': 'Nursing'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12489', 'display_name': 'Microbial Metabolites in Food Biotechnology', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/2916', 'display_name': 'Nutrition and Dietetics'}, 'field': {'id': 'https://openalex.org/fields/29', 'display_name': 'Nursing'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T10015', 'display_name': 'Genomics and Phylogenetic Studies', 'score': 0.9811, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10141', 'display_name': 'Probiotics and Fermented Foods', 'score': 0.9726, 'subfield': {'id': 'https://openalex.org/subfields/1106', 'display_name': 'Food Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/zymomonas-mobilis', 'display_name': 'Zymomonas mobilis', 'score': 0.86448765}, {'id': 'https://openalex.org/keywords/levansucrase', 'display_name': 'Levansucrase', 'score': 0.555032}, {'id': 'https://openalex.org/keywords/cloning', 'display_name': 'Cloning (programming)', 'score': 0.5455087}], 'concepts': [{'id': 'https://openalex.org/C2780604136', 'wikidata': 'https://www.wikidata.org/wiki/Q245744', 'display_name': 'Zymomonas mobilis', 'level': 4, 'score': 0.86448765}, {'id': 'https://openalex.org/C2777272437', 'wikidata': 'https://www.wikidata.org/wiki/Q131238', 'display_name': 'Bacillus subtilis', 'level': 3, 'score': 0.8097251}, {'id': 'https://openalex.org/C84148353', 'wikidata': 'https://www.wikidata.org/wiki/Q863908', 'display_name': 'Nucleic acid sequence', 'level': 3, 'score': 0.58340967}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.5802731}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.57289594}, {'id': 'https://openalex.org/C2780349071', 'wikidata': 'https://www.wikidata.org/wiki/Q30836', 'display_name': 'Levansucrase', 'level': 4, 'score': 0.555032}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.5489132}, {'id': 'https://openalex.org/C121050878', 'wikidata': 'https://www.wikidata.org/wiki/Q5135020', 'display_name': 'Cloning (programming)', 'level': 2, 'score': 0.5455087}, {'id': 'https://openalex.org/C167625842', 'wikidata': 'https://www.wikidata.org/wiki/Q899763', 'display_name': 'Peptide sequence', 'level': 3, 'score': 0.54358643}, {'id': 'https://openalex.org/C195286587', 'wikidata': 'https://www.wikidata.org/wiki/Q424689', 'display_name': 'Gene product', 'level': 4, 'score': 0.49297}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.49294972}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.29569405}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.27568924}, {'id': 'https://openalex.org/C100544194', 'wikidata': 'https://www.wikidata.org/wiki/Q41760', 'display_name': 'Fermentation', 'level': 2, 'score': 0.1386061}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.09060836}, {'id': 'https://openalex.org/C199360897', 'wikidata': 'https://www.wikidata.org/wiki/Q9143', 'display_name': 'Programming language', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C65183824', 'wikidata': 'https://www.wikidata.org/wiki/Q194361', 'display_name': 'Ethanol fuel', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': True}, {'descriptor_ui': 'D005798', 'descriptor_name': 'Genes, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': True}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D000595', 'descriptor_name': 'Amino Acid Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001407', 'descriptor_name': 'Bacillus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001483', 'descriptor_name': 'Base Sequence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003001', 'descriptor_name': 'Cloning, Molecular', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D005798', 'descriptor_name': 'Genes, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008969', 'descriptor_name': 'Molecular Sequence Data', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014176', 'descriptor_name': 'Protein Biosynthesis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D043324', 'descriptor_name': 'beta-Fructofuranosidase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1271/bbb.63.1107', 'pdf_url': 'https://academic.oup.com/bbb/article-pdf/63/6/1107/35019068/bbb1107.pdf', 'source': {'id': 'https://openalex.org/S94095188', 'display_name': 'Bioscience Biotechnology and Biochemistry', 'issn_l': '0916-8451', 'issn': ['0916-8451', '1347-6947'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/10427700', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1271/bbb.63.1107', 'pdf_url': 'https://academic.oup.com/bbb/article-pdf/63/6/1107/35019068/bbb1107.pdf', 'source': {'id': 'https://openalex.org/S94095188', 'display_name': 'Bioscience Biotechnology and Biochemistry', 'issn_l': '0916-8451', 'issn': ['0916-8451', '1347-6947'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 17, 'referenced_works': ['https://openalex.org/W1528043711', 'https://openalex.org/W1560702188', 'https://openalex.org/W1574721008', 'https://openalex.org/W1577070240', 'https://openalex.org/W1880966476', 'https://openalex.org/W1969859852', 'https://openalex.org/W1984981120', 'https://openalex.org/W1989182013', 'https://openalex.org/W1999199911', 'https://openalex.org/W2001426244', 'https://openalex.org/W2001843496', 'https://openalex.org/W2040514209', 'https://openalex.org/W2047245447', 'https://openalex.org/W2071424950', 'https://openalex.org/W2080719502', 'https://openalex.org/W2317255565', 'https://openalex.org/W2327344315'], 'related_works': ['https://openalex.org/W4239290677', 'https://openalex.org/W3025567356', 'https://openalex.org/W2215732294', 'https://openalex.org/W2097969289', 'https://openalex.org/W2092831407', 'https://openalex.org/W2067827360', 'https://openalex.org/W2048391950', 'https://openalex.org/W2004942337', 'https://openalex.org/W1935379884', 'https://openalex.org/W1213535963'], 'abstract_inverted_index': {'The': [0, 21, 46], 'β-fructofuranosidase': [1, 75], 'gene': [2], '(bff)': [3], 'from': [4], 'Bacillus': [5, 59], 'sp.': [6], 'V230': [7], 'has': [8, 18, 53], 'been': [9, 19], 'cloned': [10], 'in': [11], 'Escherichia': [12], 'coli': [13], 'and': [14, 38, 69, 74], 'its': [15], 'nucleotide': [16], 'sequence': [17, 29, 48], 'analyzed.': [20], 'product': [22, 52], 'of': [23, 26, 30, 42, 49, 57], 'bff': [24, 51], 'consists': [25], 'a': [27], 'signal': [28], '32': [31], 'amino': [32], 'acid': [33], '(a.a.)': [34], 'residues': [35, 41], 'for': [36], 'secretion': [37], '455': [39], 'a.a.': [40, 47], 'the': [43, 50, 58, 64, 70], 'extracellular': [44], 'β-fructofuranosidase.': [45], 'similarities': [54], 'with': [55], 'those': [56], 'subtilis': [60], 'levanscrase': [61, 73], '(63.7%': [62], 'identity),': [63], 'Streptococcus': [65], 'mutans': [66], 'fructosyltransferase': [67], '(33.7%),': [68], 'Zymomonas': [71], 'mobilis': [72], '(15%).': [76]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1821564040', 'counts_by_year': [{'year': 2017, 'cited_by_count': 1}], 'updated_date': '2024-12-17T03:20:21.622761', 'created_date': '2016-06-24'}