Title: [38] Immobilization of plant protoplasts by entrapment
Abstract: Plant protoplasts are used in various studies on plant-cell biochemistry and physiology. Immobilized protoplasts can also be employed to study biochemical and cellular processes. There are no standard methods for the isolation and culture of plant protoplasts. Each type of tissue and cell strain poses special problems that must be overcome by empirically adjusting the conditions used during isolation and culture. This chapter describes the preparation and immobilization of mesophyll protoplasts from Catharanthus roseus (periwinkle) and from the suspension cultured cells of Daucus carota (carrot) as representative examples. Protoplasts prepared from cultured plant cells are more stable than mesophyll protoplasts. The most extensively used method to prepare plant protoplasts is an enzymatic digestion of the cell wall under hypertonic conditions. The chapter presents the hydroxylation capacity of various preparations of D. carota protoplasts and the activity of free and carrageenan- entrapped protoplasts upon repetitive usage. The immobilized protoplasts are fully active and stabilized.
Publication Year: 1987
Publication Date: 1987-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 2
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