Title: Vitrification of cultured and non-cultured expanded and hatched porcine blastocysts.
Abstract: The aim of this experiment was to examine the survival of porcine embryos following exposure or vitrification in EFS solution. The work was carried out on cultured and non-cultured expanded and hatched blastocysts. The viability of treated embryos was assessed by their ability to develop in in vitro culture. The results showed a higher viability of cultured expanded blastocyst (35.1 percent) vitrified in EFS medium compared to those non-cultured (12.5 percent). The proportion of cultured hatched blastocyst vitrified in this medium was 13.2 percent, while none of the non-cultured hatched blastocyst survived vitrification. It is concluded that: 1. The EFS solution was more toxic to hatched blastocyst than expanded blastocyst 2. The expanded blastocyst was a more suitable developmental stage for vitrification than hatched blastocyst. Moreover, we demonstrated that the survival rate of vitrified cultured pig blastocyst was higher compared to non-cultured.
Publication Year: 2003
Publication Date: 2003-01-11
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 6
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