Title: Nitric oxide interactions with red blood cell hemoglobin: Effect of oxygenation and hematocrit
Abstract: Nitric oxide (NO) bioavailability is implicated in determining fate of many physiological and pathophysiological processes. In vasculature, nitric oxide homeostasis is maintained by its production by endothelial nitric oxide synthase and consumption by red blood cells (RBC) and leads to smooth muscle cells vasorelaxation. The reaction between NO and RBCs is under intense investigation. We studied the quantitative effects of oxygenation and hematocrit on NO – RBC interactions. For this purpose, we designed a novel stirred-bioreactor with a headspace to maintain controlled NO gas concentration. Gaseous NO was reacted with oxygenated and deoxygenated RBC suspension for a period of 10 min and samples were taken every 2 min. To study the effect of hematocrit on NO – RBC interactions, three hematocrit suspensions (5%, 20% and 45%) were reacted with NO for 10 min. Nitrite and total nitrogen species (total NOx) contents of all samples were measured by chemiluminescence assay and hemoglobin derivatives were measured using spectrophotometric analysis. Oxygenated NO–RBC interactions resulted in higher nitrite and total NOx concentrations than that of deoxygenated NO-RBC interactions. Nitrite concentrations decreased with hematocrit and total NOx concentrations increased with hematocrit. These results will enhance the present understanding of hematocrit and oxygenation effects on NO–RBC interactions. Supported by: American Heart Association National SDG #0530050N and Arkansas Biosciences Institute (ABI).
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Indexed In: ['crossref']
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