Title: Patch‐Clamp Recordings from Isolated Cardiac Myocytes
Abstract: The patch-clamp recording technique is a powerful experimental approach to investigate the functioning and the regulation of myocardial ion channels at the macroscopic and microscopic levels. This chapter describes the history and basic theory of the voltage-clamp technique to introduce patch-clamp recordings. The patch-clamp configurations developed and used for whole-cell and single-channel recordings are introduced, and the techniques and protocols typically used to achieve high-resistance "gigaohm" cardiac myocyte recording pipette seal formation and establish the various patch-clamp configurations for voltage-clamp and current-clamp recordings are described. The basic equipment, hardware, and software requirements for controlling voltage-clamp and current-clamp paradigms, data acquisition, and analyses, as well as the design of voltage- and current-clamp protocols are briefly summarized. Representative macroscopic whole-cell voltage-clamp recordings from isolated mammalian (adult C57Bl/6 mouse) ventricular myocytes are presented and analyzed for illustration purposes. Technical and experimental problems encountered with the acquisition and analyses of whole-cell patch clamp data, the limitations of the methodologies available, and the approaches typically used to correct and/or resolve these problems, are also discussed.
Publication Year: 2013
Publication Date: 2013-12-20
Language: en
Type: other
Indexed In: ['crossref']
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Cited By Count: 1
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