Title: Chapter IX Assay of Enzymes Representative of Metabolic Pathways
Abstract: This chapter discusses the assay of enzymes representative of metabolic pathways. There has been continued interest in assessing the kind and relative contribution of the various pathways of carbohydrate metabolism in microorganisms. These metabolic pathways were established mainly during the 1950s and few changes have been made since that time except for embellishments, such as the steps in the modification of a variety of carbohydrates and polyols for entry into the central pathways. The necessity of preserving enzyme activity during preparation of the extract is a major problem. Another problem is the fact that such extracts are very crude and present the maximum possibility of side reactions, which can obscure the enzyme activity under test. The probability of a major role of an enzyme is strengthened when its presence is influenced by physiological conditions. The most convincing evidence involves showing the induction of an enzyme in response to the relevant growth substrate, or the predicted change in physiological behaviour of a culture due to loss of an enzyme by mutation. The chapter concludes with a discussion of glucose and gluconate dehydrogenases.
Publication Year: 1971
Publication Date: 1971-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 22
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