Title: Use of heparinase to eliminate heparin inhibition in routine coagulation assays.
Abstract: Abstract A preparation of partially purified heparinase from Flavobacterium heparinum has been used to normalize the prothrombin times and partial thromboplastin times of plasma samples known to contain heparin. The data suggest that this effect is due entirely to the destruction of heparin, since the heparinase is free of coagulants. Furthermore, it was shown that the heparinase produces no change in the prothrombin time of factor VII-deficient plasma, nor in the partial thromboplastin times of factor IX-deficient or factor VIII-deficient plasma. The usefulness of heparinase in distinguishing between intrinsic coagulopathy and heparin contamination of plasma has been demonstrated in a series of patients. Heparinase is thus shown to be a useful reagent in the diagnostic coagulation laboratory, permitting accurate determination of prothrombin times and partial thromboplastin times on patients receiving heparin.
Publication Year: 1972
Publication Date: 1972-06-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 25
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