Title: Analysis of the development of Japanese B encephalitis (JBE) virus
Abstract: The fine structure of intracytoplasmic inclusion bodies seen in neurons and microglial cells from the thalamus of mice infected with JBE virus was studied in thin sections with the electron microscope: in many cases cells were examined in sets of sections cut in series. In the neuron fixed with the double fixation procedure, a roughly oval-shaped inclusion in the juxtanuclear region of the cytoplasm was delineated by a single-layered membrane or unit triple-layered membrane and contained spherical dense particles approximately 230 in diameter. In some infected microglial cells, the cytoplasm was almost entirely occupied by numerous inclusion bodies, the nucleus being replaced eccentrically by the bodies. The structures within the inclusion bodies were highly variable and they sometimes resembled, but were never identical with, cell organelles in the cytoplasm. It was assumed that the inclusion bodies seen in the microglial cells are products of phagocytosis since the cells are the counterparts of the macrophages of other parts of the body. It was possible to obtain information as to the presence of viral particles in the inclusion bodies by examining serial sections through neurons and microglial cells in the electron microscope. The particles, 210–250 in diameter, commonly seen within the inclusion bodies cannot be fully formed virus since mature virus particles are 370–380 in diameter.
Publication Year: 1965
Publication Date: 1965-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 12
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