Title: Qualitative and quantitative analysis of the proteins in human amniotic fluid
Abstract: Abstract The proteins were examined in specimens of human amniotic fluid obtained from 18 women in labor at term. The samples were analyzed by means of disc electrophoresis, double gel diffusion with the polyacrylamide columns and specific antisera, radial immunodiffusion, and the micro-Kjeldahl method for measuring nitrogen. The proteins produced an average of 21 bands during disc electrophoresis which were identified as prealbumins, albumin, alpha-1 globulins, alpha-2 globulins including ceruloplasmin, transferrin, immunoglobulins (Ig) A and G. In addition to identifying these proteins in all the samples of amniotic fluid, alpha-2 haptoglobin and alpha-2 macroglobulin were observed in some specimens. Amniotic fluid was shown to contain 205 mg. of protein per 100 ml. of which albumin formed 60 per cent, ceruloplasmin 1.5, transferrin 11.6, IgA 0.1, and IgG 11.0.
Publication Year: 1970
Publication Date: 1970-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 33
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