Title: Fluorescent antibody staining. 3. Preparation of fluorescein-isothiocyanate-labeled antibodies.
Abstract: Summary 1.A rapid method of preparing bright and specific staining fluorescent antibodies is described. 2.The method is discussed emphasizing a ) the need for chromatographically homogeneous antibody globulin, b ) the importance of pH in the coupling reaction and c ) the advantages of fractionating the fluorescent antibody reaction mixture on DEAE-cellulose.
Publication Year: 1965
Publication Date: 1965-08-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 265
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