Title: A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley.
Abstract: Research Article1 September 1990free access A 125 bp promoter fragment is sufficient for strong elicitor-mediated gene activation in parsley. U. van de Löcht U. van de Löcht Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author I. Meier I. Meier Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author K. Hahlbrock K. Hahlbrock Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author I. E. Somssich I. E. Somssich Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author U. van de Löcht U. van de Löcht Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author I. Meier I. Meier Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author K. Hahlbrock K. Hahlbrock Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author I. E. Somssich I. E. Somssich Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. Search for more papers by this author Author Information U. Löcht1, I. Meier1, K. Hahlbrock1 and I. E. Somssich1 1Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, Köln, FRG. The EMBO Journal (1990)9:2945-2950https://doi.org/10.1002/j.1460-2075.1990.tb07486.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info We describe the nucleotide sequence and some structural characteristics of a single copy gene encoding pathogenesis-related protein 2 (PR2) in parsley (Petroselinum crispum). Transcriptional activation of this gene in cultured parsley cells treated with fungal elicitor leads to a rapid, large and transient accumulation of PR2 mRNA. The deduced PR2 protein belongs to a novel class of evolutionarily conserved polypeptides which are closely related to disease resistance in plants. Functional analysis of a series of truncated PR2 promoter fusions with the beta-glucuronidase reporter gene, using parsley protoplasts for transient expression studies, identified a 5′ upstream element between positions −168 and −52 necessary for strong elicitor responsiveness. This small promoter fragment is active in conjunction with its own TATA box region as well as with the corresponding region from a heterologous promoter. The PR2 regulatory region exhibits no sequence similarity to any other elicitor-responsive promoter known to date. Previous ArticleNext Article Volume 9Issue 91 September 1990In this issue RelatedDetailsLoading ...