Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1584716923', 'doi': 'https://doi.org/10.1128/aem.50.3.562-567.1985', 'title': 'Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis', 'display_name': 'Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis', 'publication_year': 1985, 'publication_date': '1985-09-01', 'ids': {'openalex': 'https://openalex.org/W1584716923', 'doi': 'https://doi.org/10.1128/aem.50.3.562-567.1985', 'mag': '1584716923', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/3935048', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/238669'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/aem.50.3.562-567.1985', 'pdf_url': 'https://aem.asm.org/content/aem/50/3/562.full.pdf', 'source': {'id': 'https://openalex.org/S158228368', 'display_name': 'Applied and Environmental Microbiology', 'issn_l': '0099-2240', 'issn': ['0099-2240', '1070-6291', '1098-5336'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://aem.asm.org/content/aem/50/3/562.full.pdf', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5083352373', 'display_name': 'W. Michael Dunne', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'W M Dunne', 'raw_affiliation_strings': [], 'affiliations': []}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5050225789', 'display_name': 'Francis L. A. Buckmire', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': False, 'raw_author_name': 'F L Buckmire', 'raw_affiliation_strings': [], 'affiliations': []}], 'countries_distinct_count': 0, 'institutions_distinct_count': 0, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 5.361, 'has_fulltext': False, 'cited_by_count': 51, 'citation_normalized_percentile': {'value': 0.890134, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 93, 'max': 94}, 'biblio': {'volume': '50', 'issue': '3', 'first_page': '562', 'last_page': '567'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10869', 'display_name': 'Structure and Function of Plant Cell Walls', 'score': 0.9981, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10869', 'display_name': 'Structure and Function of Plant Cell Walls', 'score': 0.9981, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10593', 'display_name': 'Bacterial Biofilms and Quorum Sensing Mechanisms', 'score': 0.9966, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10076', 'display_name': 'Mechanisms of Plant Immune Response', 'score': 0.9948, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/alginic-acid', 'display_name': 'Alginic acid', 'score': 0.7175346}, {'id': 'https://openalex.org/keywords/strain', 'display_name': 'Strain (injury)', 'score': 0.71591866}, {'id': 'https://openalex.org/keywords/pseudomonadales', 'display_name': 'Pseudomonadales', 'score': 0.49267012}, {'id': 'https://openalex.org/keywords/root-exudates', 'display_name': 'Root Exudates', 'score': 0.49147}], 'concepts': [{'id': 'https://openalex.org/C2776938444', 'wikidata': 'https://www.wikidata.org/wiki/Q178194', 'display_name': 'Cystic fibrosis', 'level': 2, 'score': 0.82182634}, {'id': 'https://openalex.org/C2777637488', 'wikidata': 'https://www.wikidata.org/wiki/Q31856', 'display_name': 'Pseudomonas aeruginosa', 'level': 3, 'score': 0.79921305}, {'id': 'https://openalex.org/C57409179', 'wikidata': 'https://www.wikidata.org/wiki/Q1218293', 'display_name': 'Lysis', 'level': 2, 'score': 0.7251077}, {'id': 'https://openalex.org/C89423630', 'wikidata': 'https://www.wikidata.org/wiki/Q7193', 'display_name': 'Microbiology', 'level': 1, 'score': 0.720476}, {'id': 'https://openalex.org/C2778537129', 'wikidata': 'https://www.wikidata.org/wiki/Q422092', 'display_name': 'Alginic acid', 'level': 2, 'score': 0.7175346}, {'id': 'https://openalex.org/C2778022156', 'wikidata': 'https://www.wikidata.org/wiki/Q576145', 'display_name': 'Strain (injury)', 'level': 2, 'score': 0.71591866}, {'id': 'https://openalex.org/C201663137', 'wikidata': 'https://www.wikidata.org/wiki/Q21198990', 'display_name': 'Periplasmic space', 'level': 4, 'score': 0.6148514}, {'id': 'https://openalex.org/C2776738503', 'wikidata': 'https://www.wikidata.org/wiki/Q138058', 'display_name': 'Pseudomonadaceae', 'level': 4, 'score': 0.5643367}, {'id': 'https://openalex.org/C2781463711', 'wikidata': 'https://www.wikidata.org/wiki/Q131143', 'display_name': 'Pseudomonas', 'level': 3, 'score': 0.5570656}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.49629527}, {'id': 'https://openalex.org/C2781433018', 'wikidata': 'https://www.wikidata.org/wiki/Q133966', 'display_name': 'Pseudomonadales', 'level': 4, 'score': 0.49267012}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.4398982}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.43332776}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.36491174}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.35450923}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C105702510', 'wikidata': 'https://www.wikidata.org/wiki/Q514', 'display_name': 'Anatomy', 'level': 1, 'score': 0.0}, {'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.0}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D003550', 'descriptor_name': 'Cystic Fibrosis', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': True}, {'descriptor_ui': 'D011550', 'descriptor_name': 'Pseudomonas aeruginosa', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': True}, {'descriptor_ui': 'D000464', 'descriptor_name': 'Alginates', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D000464', 'descriptor_name': 'Alginates', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003550', 'descriptor_name': 'Cystic Fibrosis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004492', 'descriptor_name': 'Edetic Acid', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D004492', 'descriptor_name': 'Edetic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005954', 'descriptor_name': 'Glucosephosphate Dehydrogenase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D005954', 'descriptor_name': 'Glucosephosphate Dehydrogenase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020723', 'descriptor_name': 'Glucuronic Acid', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D006026', 'descriptor_name': 'Glycoside Hydrolases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006603', 'descriptor_name': 'Hexuronic Acids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007700', 'descriptor_name': 'Kinetics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008274', 'descriptor_name': 'Magnesium', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D008274', 'descriptor_name': 'Magnesium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008970', 'descriptor_name': 'Molecular Weight', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011189', 'descriptor_name': 'Potassium Chloride', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D011189', 'descriptor_name': 'Potassium Chloride', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011552', 'descriptor_name': 'Pseudomonas Infections', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': False}, {'descriptor_ui': 'D011552', 'descriptor_name': 'Pseudomonas Infections', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011550', 'descriptor_name': 'Pseudomonas aeruginosa', 'qualifier_ui': 'Q000502', 'qualifier_name': 'physiology', 'is_major_topic': False}, {'descriptor_ui': 'D011550', 'descriptor_name': 'Pseudomonas aeruginosa', 'qualifier_ui': 'Q000302', 'qualifier_name': 'isolation & purification', 'is_major_topic': False}, {'descriptor_ui': 'D011550', 'descriptor_name': 'Pseudomonas aeruginosa', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012965', 'descriptor_name': 'Sodium Chloride', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D012965', 'descriptor_name': 'Sodium Chloride', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013379', 'descriptor_name': 'Substrate Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 4, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/aem.50.3.562-567.1985', 'pdf_url': 'https://aem.asm.org/content/aem/50/3/562.full.pdf', 'source': {'id': 'https://openalex.org/S158228368', 'display_name': 'Applied and Environmental Microbiology', 'issn_l': '0099-2240', 'issn': ['0099-2240', '1070-6291', '1098-5336'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc238669', 'pdf_url': 'https://europepmc.org/articles/pmc238669?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC238669', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/3935048', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1128/aem.50.3.562-567.1985', 'pdf_url': 'https://aem.asm.org/content/aem/50/3/562.full.pdf', 'source': {'id': 'https://openalex.org/S158228368', 'display_name': 'Applied and Environmental Microbiology', 'issn_l': '0099-2240', 'issn': ['0099-2240', '1070-6291', '1098-5336'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320263', 'host_organization_name': 'American Society for Microbiology', 'host_organization_lineage': ['https://openalex.org/P4310320263'], 'host_organization_lineage_names': ['American Society for Microbiology'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 36, 'referenced_works': ['https://openalex.org/W1495619660', 'https://openalex.org/W1498245652', 'https://openalex.org/W1518694802', 'https://openalex.org/W1529339723', 'https://openalex.org/W1577036138', 'https://openalex.org/W1599664687', 'https://openalex.org/W1605964161', 'https://openalex.org/W1625660792', 'https://openalex.org/W1757304708', 'https://openalex.org/W177718912', 'https://openalex.org/W1861600806', 'https://openalex.org/W1897469112', 'https://openalex.org/W1899297217', 'https://openalex.org/W1975138044', 'https://openalex.org/W1996908059', 'https://openalex.org/W2013154013', 'https://openalex.org/W2019263194', 'https://openalex.org/W2024690154', 'https://openalex.org/W2033614693', 'https://openalex.org/W2048059523', 'https://openalex.org/W2049877229', 'https://openalex.org/W2073521181', 'https://openalex.org/W2078510075', 'https://openalex.org/W2081306834', 'https://openalex.org/W2082341351', 'https://openalex.org/W2085167620', 'https://openalex.org/W2143054625', 'https://openalex.org/W2163182389', 'https://openalex.org/W2221213310', 'https://openalex.org/W2320203601', 'https://openalex.org/W2418907962', 'https://openalex.org/W2940907974', 'https://openalex.org/W2972853984', 'https://openalex.org/W4247241355', 'https://openalex.org/W4295845553', 'https://openalex.org/W89510312'], 'related_works': ['https://openalex.org/W4234581120', 'https://openalex.org/W4232722969', 'https://openalex.org/W2518443735', 'https://openalex.org/W2434622820', 'https://openalex.org/W2241183597', 'https://openalex.org/W2160082880', 'https://openalex.org/W2056426580', 'https://openalex.org/W2054179773', 'https://openalex.org/W1999535863', 'https://openalex.org/W1977357647'], 'abstract_inverted_index': {'An': [0], 'exopolysaccharide': [1, 29], 'depolymerase': [2, 20, 83, 121, 135], 'was': [3], 'isolated': [4], 'from': [5, 30, 110], 'a': [6, 71], 'mucoid': [7, 105], 'strain': [8, 33], 'of': [9, 12, 18, 67, 73, 90, 93, 103, 125, 129], 'Pseudomonas': [10], 'aeruginosa': [11, 107], 'cystic': [13, 113], 'fibrosis': [14, 114], 'origin.': [15], 'Purified': [16], 'preparations': [17], 'the': [19, 25, 31, 46, 56, 65, 74, 88, 126, 130], 'showed': [21, 115], 'maximum': [22], 'activity': [23, 36, 122], 'against': [24, 37], 'unacetylated': [26], 'polymannuronic': [27], 'acid': [28], 'same': [32], 'and': [34, 60], 'little': [35], 'commercially': [38], 'prepared': [39], 'alginic': [40], 'acid.': [41], 'The': [42, 82], 'evidence': [43], 'suggests': [44], 'that': [45, 116], 'enzyme': [47], 'is': [48, 61, 84], 'either': [49], 'periplasmic': [50], 'in': [51, 64], 'location': [52], 'or': [53], 'associated': [54], 'with': [55, 112], 'outer': [57], 'cell': [58, 68], 'membrane': [59], 'released': [62, 86], 'extracellularly,': [63], 'absence': [66], 'lysis,': [69], 'after': [70, 87], 'reduction': [72], 'culture': [75], 'magnesium': [76], '(Mg2+)': [77], 'concentration': [78], 'below': [79], '3.0': [80, 98], 'mM.': [81], 'also': [85], 'addition': [89], 'sublethal': [91], 'concentrations': [92], 'EDTA': [94], 'to': [95], 'cultures': [96], 'containing': [97], 'mM': [99], 'Mg2+.': [100], 'A': [101], 'survey': [102], 'additional': [104], 'P.': [106], 'isolates': [108], 'recovered': [109], 'patients': [111], 'nearly': [117], '60%': [118], 'demonstrated': [119], 'similar': [120], 'while': [123], 'none': [124], 'nonmucoid': [127], 'revertants': [128], 'parent': [131], 'strains': [132], 'produced': [133], 'detectable': [134], 'activity.': [136]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1584716923', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 1}], 'updated_date': '2024-08-15T11:48:19.844665', 'created_date': '2016-06-24'}