Publication Information

{'id': 'https://openalex.org/W1581139356', 'doi': None, 'title': 'Electron Cryo-Tomography of Vitreous Cryo-Sections: Towards Imaging Biological Nanomachines in their Cellular Environment', 'display_name': 'Electron Cryo-Tomography of Vitreous Cryo-Sections: Towards Imaging Biological Nanomachines in their Cellular Environment', 'publication_year': 2011, 'publication_date': '2011-04-20', 'ids': {'openalex': 'https://openalex.org/W1581139356', 'mag': '1581139356'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'http://repository.tudelft.nl/assets/uuid:720a11b2-8d67-4463-b7cf-be465441bb2e/Pierson_Thesis_Final_repository.pdf', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': [], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5112472000', 'display_name': 'J. Pierson', 'orcid': None}, 'institutions': [], 'countries': [], 'is_corresponding': True, 'raw_author_name': 'J. Pierson', 'raw_affiliation_strings': [], 'affiliations': []}], 'institution_assertions': [], 'countries_distinct_count': 0, 'institutions_distinct_count': 0, 'corresponding_author_ids': ['https://openalex.org/A5112472000'], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': False, 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 65}, 'biblio': {'volume': None, 'issue': None, 'first_page': None, 'last_page': None}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10857', 'display_name': 'Advanced Electron Microscopy Techniques and Applications', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1315', 'display_name': 'Structural Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10857', 'display_name': 'Advanced Electron Microscopy Techniques and Applications', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1315', 'display_name': 'Structural Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10303', 'display_name': 'Photosynthetic Processes and Mechanisms', 'score': 0.9932, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12039', 'display_name': 'Electron and X-Ray Spectroscopy Techniques', 'score': 0.9796, 'subfield': {'id': 'https://openalex.org/subfields/2508', 'display_name': 'Surfaces, Coatings and Films'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/cryo-electron-tomography', 'display_name': 'Cryo-electron tomography', 'score': 0.85464}, {'id': 'https://openalex.org/keywords/cryo-electron-microscopy', 'display_name': 'Cryo-Electron Microscopy', 'score': 0.72762537}, {'id': 'https://openalex.org/keywords/microtome', 'display_name': 'Microtome', 'score': 0.62237126}, {'id': 'https://openalex.org/keywords/biological-specimen', 'display_name': 'Biological specimen', 'score': 0.5038938}, {'id': 'https://openalex.org/keywords/optical-sectioning', 'display_name': 'Optical sectioning', 'score': 0.4360265}], 'concepts': [{'id': 'https://openalex.org/C2777993257', 'wikidata': 'https://www.wikidata.org/wiki/Q904576', 'display_name': 'Cryo-electron tomography', 'level': 3, 'score': 0.85464}, {'id': 'https://openalex.org/C20702342', 'wikidata': 'https://www.wikidata.org/wiki/Q5190506', 'display_name': 'Cryo-electron microscopy', 'level': 2, 'score': 0.72762537}, {'id': 'https://openalex.org/C75806775', 'wikidata': 'https://www.wikidata.org/wiki/Q5358194', 'display_name': 'Electron tomography', 'level': 4, 'score': 0.6687051}, {'id': 'https://openalex.org/C680638', 'wikidata': 'https://www.wikidata.org/wiki/Q898419', 'display_name': 'Microtome', 'level': 2, 'score': 0.62237126}, {'id': 'https://openalex.org/C64389972', 'wikidata': 'https://www.wikidata.org/wiki/Q2075980', 'display_name': 'Biological specimen', 'level': 2, 'score': 0.5038938}, {'id': 'https://openalex.org/C163716698', 'wikidata': 'https://www.wikidata.org/wiki/Q841267', 'display_name': 'Tomography', 'level': 2, 'score': 0.47425404}, {'id': 'https://openalex.org/C171250308', 'wikidata': 'https://www.wikidata.org/wiki/Q11468', 'display_name': 'Nanotechnology', 'level': 1, 'score': 0.45427972}, {'id': 'https://openalex.org/C138268822', 'wikidata': 'https://www.wikidata.org/wiki/Q1051925', 'display_name': 'Resolution (logic)', 'level': 2, 'score': 0.45148143}, {'id': 'https://openalex.org/C20446124', 'wikidata': 'https://www.wikidata.org/wiki/Q7098895', 'display_name': 'Optical sectioning', 'level': 3, 'score': 0.4360265}, {'id': 'https://openalex.org/C93877712', 'wikidata': 'https://www.wikidata.org/wiki/Q132560', 'display_name': 'Electron microscope', 'level': 2, 'score': 0.42554834}, {'id': 'https://openalex.org/C192562407', 'wikidata': 'https://www.wikidata.org/wiki/Q228736', 'display_name': 'Materials science', 'level': 0, 'score': 0.3900544}, {'id': 'https://openalex.org/C136229726', 'wikidata': 'https://www.wikidata.org/wiki/Q327092', 'display_name': 'Biomedical engineering', 'level': 1, 'score': 0.3863989}, {'id': 'https://openalex.org/C147080431', 'wikidata': 'https://www.wikidata.org/wiki/Q1074953', 'display_name': 'Microscopy', 'level': 2, 'score': 0.33914244}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.31372607}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.29039216}, {'id': 'https://openalex.org/C146088050', 'wikidata': 'https://www.wikidata.org/wiki/Q744818', 'display_name': 'Transmission electron microscopy', 'level': 2, 'score': 0.21332487}, {'id': 'https://openalex.org/C120665830', 'wikidata': 'https://www.wikidata.org/wiki/Q14620', 'display_name': 'Optics', 'level': 1, 'score': 0.19298306}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.16668269}, {'id': 'https://openalex.org/C121332964', 'wikidata': 'https://www.wikidata.org/wiki/Q413', 'display_name': 'Physics', 'level': 0, 'score': 0.13335589}, {'id': 'https://openalex.org/C154945302', 'wikidata': 'https://www.wikidata.org/wiki/Q11660', 'display_name': 'Artificial intelligence', 'level': 1, 'score': 0.12795004}, {'id': 'https://openalex.org/C193016168', 'wikidata': 'https://www.wikidata.org/wiki/Q874835', 'display_name': 'Scanning transmission electron microscopy', 'level': 3, 'score': 0.1257025}, {'id': 'https://openalex.org/C127413603', 'wikidata': 'https://www.wikidata.org/wiki/Q11023', 'display_name': 'Engineering', 'level': 0, 'score': 0.08560261}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'http://repository.tudelft.nl/assets/uuid:720a11b2-8d67-4463-b7cf-be465441bb2e/Pierson_Thesis_Final_repository.pdf', 'pdf_url': None, 'source': None, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 17, 'referenced_works': ['https://openalex.org/W1633531403', 'https://openalex.org/W1978485708', 'https://openalex.org/W1982729399', 'https://openalex.org/W1993074961', 'https://openalex.org/W2028605946', 'https://openalex.org/W2036765062', 'https://openalex.org/W2088317150', 'https://openalex.org/W2098795972', 'https://openalex.org/W2103999808', 'https://openalex.org/W2106393979', 'https://openalex.org/W2117756276', 'https://openalex.org/W2121449107', 'https://openalex.org/W2122390465', 'https://openalex.org/W2125214575', 'https://openalex.org/W2143888505', 'https://openalex.org/W2169882798', 'https://openalex.org/W64861853'], 'related_works': ['https://openalex.org/W92111409', 'https://openalex.org/W3114541747', 'https://openalex.org/W3005702700', 'https://openalex.org/W3005351590', 'https://openalex.org/W2990465796', 'https://openalex.org/W2965314492', 'https://openalex.org/W2924863631', 'https://openalex.org/W2921973809', 'https://openalex.org/W2905997661', 'https://openalex.org/W2889454102', 'https://openalex.org/W2794960104', 'https://openalex.org/W2776294243', 'https://openalex.org/W2766732551', 'https://openalex.org/W2725604878', 'https://openalex.org/W2722741161', 'https://openalex.org/W2606754372', 'https://openalex.org/W2605810930', 'https://openalex.org/W2216533205', 'https://openalex.org/W2186391941', 'https://openalex.org/W1966176589'], 'abstract_inverted_index': {'The': [0, 68, 540], 'field': [1], 'of': [2, 18, 56, 86, 98, 108, 123, 138, 149, 170, 194, 201, 232, 257, 266, 274, 295, 303, 317, 324, 327, 348, 369, 380, 388, 458, 486, 496, 543, 584, 611, 628, 652, 658, 687, 696], 'biological': [3], 'electron': [4, 50, 174, 325], 'microcopy': [5], '(EM)': [6], 'has': [7], 'evolved': [8], 'into': [9, 564, 614], 'a': [10, 42, 62, 82, 92, 177, 191, 280, 310, 315, 332, 376, 420, 433, 490, 568, 662, 685], 'reliable': [11, 336], 'imaging': [12, 159], 'technique': [13, 70, 79, 137, 169, 323, 387, 495, 657], 'for': [14, 155, 173, 196, 269, 338, 502, 506], 'examining': [15], 'the': [16, 53, 66, 76, 121, 136, 168, 198, 213, 217, 220, 230, 245, 251, 255, 258, 264, 267, 275, 287, 293, 301, 304, 318, 322, 344, 363, 371, 386, 417, 427, 445, 454, 465, 484, 494, 522, 526, 549, 556, 565, 585, 589, 592, 603, 609, 615, 622, 625, 629, 635, 644, 650, 653, 656, 671, 675, 681, 694, 697], 'ultrastructure': [17, 302], 'cells': [19, 87, 112, 124, 146, 476, 508, 532], 'and': [20, 60, 88, 147, 158, 205, 224, 248, 335, 477, 509, 607, 647, 666], 'their': [21, 89, 478, 612], 'constituents': [22, 90, 479], 'at': [23, 453, 621], 'molecular,': [24], 'and,': [25], 'in': [26, 91, 185, 229, 309, 405, 419, 489, 515, 558, 700], 'some': [27], 'cases,': [28], 'atomic1,': [29], '2': [30], 'resolution.': [31], 'Conventional': [32], 'EM': [33, 58, 187, 222, 288], 'techniques': [34, 524], 'are': [35, 125, 152, 357, 480, 553], 'being': [36], 'overshadowed': [37], 'by': [38, 106, 299, 366, 397, 432, 444, 470, 483, 555, 674], 'cryo-techniques,': [39], 'which': [40], 'ensure': [41], 'more': [43, 333], 'native': [44], 'cellular': [45, 312, 341], 'preparation': [46], 'method.': [47], 'In': [48, 211, 235, 423, 517, 575, 591], 'addition,': [49, 212], 'tomography': [51, 73], 'eliminates': [52], 'projection': [54], 'problem': [55], 'single': [57], 'images': [59], 'adds': [61], 'third': [63], 'dimension': [64], 'to': [65, 80, 128, 143, 183, 208, 244, 262, 282, 286, 359, 529, 547, 560, 692, 702], 'analysis.': [67], 'cryo-preparation': [69], 'combined': [71], 'with': [72, 226, 537, 588, 643], 'is': [74, 176, 330, 391, 632], 'currently': [75], 'most': [77], 'suitable': [78], 'enable': [81], 'direct': [83], 'three-dimensional': [84], 'observation': [85], 'close-to-native': [93], 'environment.': [94, 313], 'Although': [95, 385], 'several': [96], 'aspects': [97], 'cell': [99, 428, 698], 'biology': [100], 'have': [101, 163, 403, 534, 601], 'profited': [102], 'from': [103, 525], 'information': [104], 'obtained': [105], 'cryo-ET': [107], 'plunge': [109, 132], 'frozen': [110], 'whole': [111], 'preparations': [113], 'or': [114, 117], 'isolated': [115], 'organelles': [116], 'molecular': [118], 'complexes': [119, 353, 463], 'studies,': [120], 'majority': [122, 610], 'too': [126, 153], 'large': [127, 145, 154, 351, 461, 507], 'freeze': [129], 'using': [130], 'conventional': [131, 156], 'freezing': [133, 157], 'techniques.': [134, 160], 'Therefore,': [135, 493], 'vitreous': [139, 171, 202, 276, 328, 389, 412, 497, 659], 'cryo-sectioning': [140, 172, 329, 390, 498, 660], 'was': [141, 181, 409, 452, 546], 'introduced': [142], 'incorporate': [144], 'pieces': [148], 'tissue': [150], 'that': [151, 180, 411, 475, 511, 533, 552, 570, 620, 680, 689], 'As': [161, 314], 'we': [162, 238, 278, 402, 449, 520, 579, 594, 668], 'seen': [164, 404], 'throughout': [165], 'this': [166, 544], 'thesis,': [167], 'cryo-tomography': [175, 326], 'developing': [178], 'method': [179, 281, 337, 665], 'difficult': [182], 'implement': [184], 'many': [186], 'labs.': [188], 'It': [189, 408], 'requires': [190], 'high': [192], 'level': [193], 'dexterity': [195], 'manipulating': [197], 'thin': [199], 'ribbon': [200], 'cryo': [203], 'sections': [204, 225, 285], 'transferring': [206], 'it': [207], 'an': [209, 367], 'EM.': [210], 'contamination': [214, 228, 249], 'levels': [215], 'within': [216, 250, 383, 649], 'microtome': [218], 'coat': [219], 'equipment,': [221], 'grid,': [223], 'water': [227], 'form': [231], 'ice': [233], 'crystals.': [234], 'Chapter': [236, 406, 518], '3': [237], 'addressed': [239], 'these': [240, 296], 'limitations;': [241], 'section': [242], 'attachment': [243], 'support': [246, 289], 'film': [247], 'cryo-ultramicrotome.': [252], 'By': [253], 'altering': [254], 'output': [256], 'electrode,': [259], 'normally': [260], 'used': [261], 'ionize': [263], 'surface': [265, 271], 'diamond': [268], 'better': [270], 'gliding': [272], 'properties': [273], 'cryo-sections,': [277], 'developed': [279], 'electrostatically': [283], 'attach': [284], 'film.': [290], 'We': [291, 473, 618, 638, 677], 'illustrated': [292], 'advantage': [294], 'novel': [297, 319, 523], 'developments': [298], 'exploring': [300, 503], '80S': [305, 355, 466], 'Saccharomyces': [306], 'cerevisiae': [307], 'ribosome': [308], 'close-to-native,': [311], 'result': [316], 'methods': [320], 'developed,': [321], 'becoming': [331], 'widely': [334], 'ultra': [339], 'structural': [340, 550], 'studies.': [342], 'At': [343], 'current': [345, 455], 'resolution': [346, 364, 456], 'limit': [347, 457], '4-5': [349, 459], 'nm,': [350, 460], 'macromolecular': [352, 462, 504], 'like': [354, 464], 'ribosomes': [356, 467], 'easy': [358], 'recognize.': [360], 'However': [361], 'as': [362, 401], 'increases': [365], 'order': [368, 559, 701], 'magnitude,': [370], 'near': [372], 'future': [373], 'should': [374], 'allow': [375], 'full': [377], 'comprehensive': [378], 'description': [379], 'supramolecular': [381], 'architecture': [382], 'cells.': [384], 'gaining': [392], 'importance': [393], 'it’s': [394], 'still': [395], 'plagued': [396], 'section-induced': [398, 471, 487], 'cutting': [399], 'artifacts,': [400], '4.': [407], 'thought': [410], 'cryo-section': [413], 'induced': [414], 'compression': [415, 488], 'affected': [416, 482], 'specimen': [418], 'homogeneous': [421], 'manner.': [422, 492], 'other': [424], 'words,': [425], 'if': [426], 'would': [429, 441], 'be': [430, 442, 513], 'compressed': [431, 443], 'given': [434], 'value': [435], 'then': [436], 'all': [437], 'its': [438, 562, 704], 'intracellular': [439], 'components': [440, 551], 'same': [446], 'value.': [447], 'What': [448], 'observed,': [450], 'however,': [451, 679], 'remained': [468], 'unaltered': [469], 'compression.': [472], 'concluded': [474], 'not': [481], 'forces': [485], 'uniform': [491], 'will': [499], 'remain': [500], 'valuable': [501], 'landscapes': [505], 'tissues': [510], 'cannot': [512, 669], 'imaged': [514], 'toto.': [516], '5': [519], 'applied': [521], 'previous': [527], 'studies': [528], 'Escherichia': [530], 'coli': [531], 'been': [535, 573], 'infected': [536], 'bacteriophage': [538], 'T7.': [539], 'main': [541], 'goal': [542], 'study': [545, 593], 'visualize': [548], 'assembled': [554, 602], 'phage': [557, 682], 'transfer': [561, 605, 703], 'genome': [563, 604, 613], 'host': [566, 616], 'cell,': [567], 'feat': [569], 'had': [571], 'never': [572], 'achieved.': [574], 'our': [576], 'resulting': [577], 'observations': [578], 'found': [580], 'three': [581], 'distinct': [582], 'phenotypes': [583], 'capsid': [586], 'interacting': [587], 'cell.': [590, 617], 'focused': [595], 'on': [596], 'empty': [597], 'capsids': [598], 'because': [599], 'they': [600], 'complex': [606, 686], 'expelled': [608, 673], 'observe': [619], 'infection': [623], 'site,': [624], 'outer': [626, 645], 'membrane': [627, 646, 699], 'bacterial': [630], 'envelope': [631, 651], 'pinched': [633], 'towards': [634], 'inner': [636], 'membrane.': [637], 'also': [639, 648], 'observed': [640], 'proteins': [641, 672, 688], 'associated': [642], 'bacteria.': [654], 'Unfortunately': [655], 'lacks': [661], 'protein': [663], 'localization': [664], 'therefore': [667], 'identify': [670], 'bacteriophage.': [676], 'conclude,': [678], 'must': [683], 'construct': [684], 'work': [690], 'together': [691], 'reduce': [693], 'distance': [695], 'genome.': [705]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1581139356', 'counts_by_year': [], 'updated_date': '2024-12-14T10:34:29.349265', 'created_date': '2016-06-24'}