Title: 6.12 Detection, Purification, and Synthesis of n-acylhomoserine Lactone Quorum Sensing Signal Molecules
Abstract: This chapter describes the most relevant practical approaches for the identification and characterization of the N-acylhomoserine lactones (AHL) family of quorum sensing signal molecules. The first indication of AHL-mediated gene regulation in a Gram-negative bacterial pathogen is the appearance of a specific phenotype in a cell density dependent fashion. This may be further evidenced by an earlier manifestation of that phenotype, when the organism is grown in the presence of some of its own filtered spent culture supernatant as a potential source of AHLs or indeed other chemically distinct signal molecules. The development of sensitive bioassays for the detection of AHLs has greatly facilitated the screening of micro-organisms for new AHL molecules. N-Acyl-L-homoserine lactones can be prepared by a carbodiimide-mediated acylation of L-homoserine lactone hydrochloride. A typical procedure is explained in the chapter.
Publication Year: 1998
Publication Date: 1998-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 51
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