Publication Information

{'id': 'https://openalex.org/W1559514166', 'doi': 'https://doi.org/10.1074/jbc.273.34.22091', 'title': 'The α1(VIII) and α2(VIII) Chains of Type VIII Collagen Can Form Stable Homotrimeric Molecules', 'display_name': 'The α1(VIII) and α2(VIII) Chains of Type VIII Collagen Can Form Stable Homotrimeric Molecules', 'publication_year': 1998, 'publication_date': '1998-08-01', 'ids': {'openalex': 'https://openalex.org/W1559514166', 'doi': 'https://doi.org/10.1074/jbc.273.34.22091', 'mag': '1559514166', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9705353'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.273.34.22091', 'pdf_url': 'http://www.jbc.org/article/S0021925818488886/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925818488886/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5072307332', 'display_name': 'Chris Illidge', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I2800776015', 'display_name': 'Wellcome Centre for Cell-Matrix Research', 'ror': 'https://ror.org/0094tm228', 'country_code': 'GB', 'type': 'facility', 'lineage': ['https://openalex.org/I2800776015', 'https://openalex.org/I28407311', 'https://openalex.org/I87048295']}, {'id': 'https://openalex.org/I28407311', 'display_name': 'University of Manchester', 'ror': 'https://ror.org/027m9bs27', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I28407311']}], 'countries': ['GB'], 'is_corresponding': False, 'raw_author_name': 'Chris Illidge', 'raw_affiliation_strings': ['University of Manchester, Wellcome Trust Center for Cell/Matrix Research, Manchester M13 9PT, United Kingdom'], 'affiliations': [{'raw_affiliation_string': 'University of Manchester, Wellcome Trust Center for Cell/Matrix Research, Manchester M13 9PT, United Kingdom', 'institution_ids': ['https://openalex.org/I2800776015', 'https://openalex.org/I28407311']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5071497079', 'display_name': 'Cay M. 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4336, 4363, 4366, 4391, 4407, 4418, 4422, 4436, 4559, 4611], 'composition': [19, 137, 163, 281, 595, 953, 3387, 3550, 3557, 3574], 'of': [20, 56, 68, 79, 108, 111, 127, 164, 200, 212, 223, 252, 255, 271, 293, 298, 307, 478, 484, 596, 625, 640, 734, 857, 862, 945, 994, 1004, 1007, 1032, 1074, 1281, 1325, 1347, 1354, 1360, 1422, 1434, 1462, 1467, 1472, 1526, 1544, 1548, 1558, 1569, 1576, 1583, 1631, 1660, 1674, 1750, 1814, 1867, 1884, 1901, 1937, 1952, 1958, 1972, 1977, 1983, 2018, 2033, 2080, 2122, 2125, 2134, 2141, 2155, 2185, 2195, 2234, 2245, 2282, 2343, 2391, 2424, 2452, 2490, 2540, 2677, 2687, 2724, 2735, 2751, 2769, 2785, 2886, 2914, 2947, 2952, 2982, 2996, 3045, 3070, 3110, 3123, 3147, 3162, 3164, 3176, 3187, 3211, 3219, 3230, 3249, 3257, 3306, 3360, 3388, 3488, 3504, 3572, 3580, 3623, 3778, 3784, 3811, 3821, 3883, 3923, 3930, 3937, 3945, 3955, 3961, 3999, 4013, 4038, 4061, 4063, 4103, 4206, 4212, 4224, 4249, 4269, 4274, 4323, 4330, 4365, 4438, 4572, 4589], 'type': [21, 112, 165, 256, 597, 626, 727, 1130, 2160, 2833, 3338, 3378, 3439, 3480, 3511, 3543, 3660, 4439], 'far': [25, 169, 601], 'from': [26, 170, 602, 1043, 1059, 1070, 1093, 1113, 1139, 1150, 1162, 1173, 1182, 1194, 1205, 1286, 1366, 1390, 1402, 1555, 1645, 2083, 2144, 2198, 2237, 2284, 2427, 3952], 'resolved.': [27, 171, 603], 'To': [28, 172], 'address': [29, 173, 3569], 'this': [30, 128, 174, 272, 946, 998, 2010, 3814, 3968, 4216, 4309], 'question,': [31, 175], 'we': [32, 176, 1000], 'expressed': [34, 178, 4452], 'full-length': [35, 179, 1005, 1279, 1436, 1812, 2431, 3600, 3678, 3897], 'α2(VIII)': [38, 102, 182, 246, 490, 617, 1029, 1377, 1531, 1584, 1638, 2434, 2498, 2511, 2782, 3015, 3062, 3135, 3191, 3234, 3294, 3603, 3885, 3898, 3905, 3938, 4337], 'in': [40, 42, 138, 184, 186, 282, 722, 739, 813, 821, 825, 894, 957, 971, 1011, 1013, 1105, 1214, 1245, 1426, 1737, 1743, 1765, 1808, 1817, 1877, 1927, 1990, 2030, 2061, 2076, 2242, 2319, 2436, 2438, 2459, 2537, 2722, 2757, 2761, 2880, 2884, 3035, 3073, 3105, 3173, 3237, 3375, 3555, 3588, 3590, 3608, 3843, 3979, 4010, 4110, 4176, 4233, 4305, 4354, 4362, 4372, 4406, 4417, 4421, 4433, 4453], 'an': [41, 185, 1012, 1566, 2437, 3589, 3884, 4246], 'vitro': [43, 187, 972, 1106, 2439, 3238, 3591, 3609], 'translation': [44, 54, 188, 198, 1102, 2084, 2145, 2199, 2238, 2440, 2733, 2818, 3016, 3071, 3185, 3592, 4235, 4612], 'system': [45, 189, 1015, 2441, 4236], 'supplemented': [46, 190, 1016, 1226, 2442, 4237], 'with': [47, 191, 1017, 1227, 1250, 1299, 1442, 1499, 1533, 1601, 1705, 1719, 1839, 1896, 2043, 2088, 2152, 2203, 2224, 2324, 2443, 2806, 3020, 3091, 3261, 3384, 3546, 4238, 4457, 4581], 'semipermeabilized': [48, 192, 1019, 2445, 2678, 4207], 'cells.': [49, 193], 'Both': [50, 194, 2508, 2779, 3901], 'gave': [52, 196, 2449], 'product': [55, 67, 199, 211, 306, 1575, 3072, 3305, 3487], '∼80': [57, 201, 1344, 2453], 'kDa': [58, 202, 2454, 2845, 2916, 3308], 'that': [59, 72, 100, 121, 131, 203, 216, 244, 265, 275, 300, 482, 726, 740, 890, 965, 1025, 1730, 2108, 2562, 2917, 2975, 3130, 3209, 3337, 3374, 3501, 3599, 3611, 3641, 3676, 3890, 3965, 3989, 4231, 4299, 4315, 4321, 4342, 4400, 4410, 4449, 4573, 4579], 'could': [60, 75, 204, 219, 3057, 3790, 3939], 'be': [61, 124, 205, 268, 379, 748, 1799, 2720, 2746, 2823, 3003, 3058, 3328, 3485, 3605, 3791, 3893, 3910, 3940, 4173, 4568, 4586], 'shown': [62, 206, 377, 632, 2682, 2821, 3168, 3675, 3792, 3878, 3908, 4071], 'to': [63, 115, 140, 207, 259, 284, 378, 488, 621, 635, 807, 896, 1302, 1319, 1342, 1416, 1496, 1542, 1564, 1594, 1628, 1652, 1740, 1759, 1798, 1845, 1919, 2015, 2272, 2279, 2286, 2304, 2485, 2503, 2514, 2551, 2672, 2683, 2719, 2748, 2772, 2810, 2822, 2828, 2847, 2986, 3048, 3096, 3154, 3254, 3327, 3392, 3394, 3438, 3484, 3538, 3568, 3583, 3630, 3635, 3644, 3654, 3659, 3793, 3846, 3909, 3917, 3942, 4065, 4172, 4398, 4428, 4561], 'produce': [64, 208, 3194, 3542], 'chymotrypsin/trypsin-resistant': [66, 210, 3935], '∼60': [69, 213, 3307], 'kDa,': [70, 214], 'indicating': [71, 215, 3042, 3208], 'both': [73, 109, 217, 253, 613, 820, 1026, 1803, 1850, 2428, 3011, 3036, 3046, 3131, 3250, 3677, 3822, 4344], 'form': [76, 116, 220, 260, 969, 2515, 3049, 3395, 3584, 3636, 4303, 4464, 4562], 'homotrimers.': [77, 221, 1035], 'Hydroxylation': [78, 222], 'proline': [80, 224, 3165, 4000, 4064, 4324, 4415], 'residues': [81, 225, 3166, 4109], 'was': [82, 96, 103, 226, 240, 247, 301, 990, 1160, 1180, 1203, 1283, 1296, 1340, 1364, 1384, 1414, 1586, 1685, 1696, 1823, 1915, 2013, 2110, 2117, 2180, 2222, 2462, 2740, 2861, 2874, 2905, 3025, 3088, 3214, 3239, 3277, 3319, 4277, 4349], 'prerequisite': [84, 228], 'for': [85, 92, 101, 229, 236, 245, 1654, 1871, 1987, 2003, 2068, 2113, 2149, 2266, 2363, 2369, 2386, 2464, 2555, 2572, 2944, 3115, 3119, 3141, 3216, 3282, 3293, 3344, 3840, 3873, 3896, 4335, 4343, 4468, 4606], 'stable': [86, 117, 230, 261, 1034, 2533, 2862, 2948, 3222, 3585, 3616, 3643, 3686, 3991, 4316, 4564], 'trimer': [87, 231], 'formation.': [88, 232, 3144], 'The': [89, 106, 233, 250, 1049, 1098, 1156, 1165, 1507, 1830, 2178, 2479, 2675, 2817, 2859, 3145, 3228, 3300, 3369, 3594, 3619, 3803, 3928, 4036, 4059, 4204, 4328, 4575], 'melting': [90, 234], 'temperature': [91, 235, 3245], 'homotrimer': [95, 239], '45': [97, 241, 3280, 4275], '°C,': [98, 242, 2288, 2878, 3648, 4226], 'whereas': [99, 243, 3449], '42': [104, 248, 3291, 4331], '°C.': [105, 249, 2008, 2547, 3227, 3656], 'ability': [107, 251, 3044, 3579, 4560], 'triple': [118, 262, 610, 2949, 3051, 3142, 3223, 3586, 3911, 3981, 3992, 4040], 'helices': [119, 263, 2981, 3224, 3457, 3587], 'suggests': [120, 264, 3373], 'there': [122, 266, 3355, 3891, 4357, 4370, 4411], 'may': [123, 133, 267, 277, 3892, 3974, 4463, 4585], 'different': [125, 141, 269, 285, 4582, 4595], 'forms': [126, 270], 'cells': [132, 276, 314, 753, 1211, 1242, 2000, 2056, 2082, 2143, 2197, 2236, 2679, 2803, 3541, 4208], 'modulate': [134, 278], 'response': [139, 283, 895], 'biological': [142, 286], 'conditions.': [143, 287, 1454, 2674], 'Collagen': [288], 'member': [292], 'class': [297], 'collagens': [299, 3823], 'initially': [302], 'detected': [303], 'as': [304, 1254, 2091, 2162, 2208, 2308, 2940, 3167, 3537, 3837, 4002], 'bovine': [308, 1815, 2406, 3323, 4218], 'aortic': [309], 'rabbit': [311, 1945], 'corneal': [312], 'endothelial': [313, 752], '(1Benya': [315], 'P.D.': [316, 347, 497, 678, 3461], 'Padilla': [317], 'S.R.': [318], 'J.': [319, 361, 399, 423, 442, 504, 528, 561, 581, 685, 709, 760, 779, 905, 1261, 1269, 1781, 2098, 2169, 2586, 2614, 2641, 2648, 2695, 2703, 2960, 3406, 3426, 3468, 3698, 3726, 3753, 3760, 4126, 4151, 4193, 4257, 4480, 4525], 'Biol.': [320, 362, 505, 529, 563, 686, 710, 762, 1782, 2587, 2615, 2649, 3408, 3469, 3699, 3727, 3761, 4127, 4152, 4195], 'Chem.': [321, 363, 506, 530, 687, 711, 1783, 2588, 2616, 2650, 3470, 3700, 3728, 3762, 4128, 4153], '1986;': [322, 547], '261:': [323, 4197], '4160-4169Abstract': [324], 'Full': [325, 367, 510, 534, 691, 715, 1787, 2592, 2594, 2620, 2622, 2654, 2656, 3474, 3704, 3706, 3732, 3734, 3766, 3768, 4132, 4134, 4157, 4159], 'Text': [326, 368, 511, 535, 692, 716, 1788, 2593, 2595, 2621, 2623, 2655, 2657, 3475, 3705, 3707, 3733, 3735, 3767, 3769, 4133, 4135, 4158, 4160], 'PDF': [327, 369, 512, 536, 693, 717, 1789, 2596, 2624, 2658, 3476, 3708, 3736, 3770, 4136, 4161], 'PubMed': [328, 341, 370, 403, 447, 513, 537, 550, 567, 587, 694, 718, 766, 785, 843, 882, 909, 937, 982, 1273, 1790, 2102, 2173, 2216, 2351, 2597, 2625, 2659, 2707, 2964, 3412, 3432, 3477, 3528, 3709, 3737, 3771, 4031, 4090, 4137, 4162, 4199, 4261, 4291, 4484, 4512, 4531, 4553], 'Google': [329, 344, 353, 371, 406, 429, 450, 465, 514, 538, 553, 570, 590, 695, 719, 769, 788, 846, 885, 912, 940, 985, 1276, 1791, 2105, 2176, 2219, 2354, 2600, 2628, 2662, 2710, 2967, 3415, 3435, 3478, 3531, 3712, 3740, 3774, 3867, 4034, 4093, 4140, 4165, 4202, 4264, 4294, 4487, 4515, 4532, 4556], 'Scholar,': [330, 345, 354, 407, 430, 451, 515, 539, 554, 571, 696, 847, 867, 913, 2601, 2629, 3416, 3713, 3741, 4488, 4516, 4533], '2Sage': [331], 'H.': [332, 356, 415, 453, 556, 558, 755, 757, 849, 927, 3401, 3403, 4502], 'Pritzl': [333], 'P.': [334, 336, 360, 459, 543, 4518, 4522], 'Bornstein': [335, 359, 458, 542], 'Biochemistry.': [337, 546], '1980;': [338, 350], '19:': [339, 3863], '5747-5755Crossref': [340], 'Scopus': [342, 404, 448, 551, 568, 588, 767, 786, 844, 883, 910, 938, 983, 1274, 2103, 2174, 2217, 2352, 2598, 2626, 2660, 2708, 2965, 3413, 3433, 3529, 3710, 3738, 3772, 3865, 4032, 4091, 4138, 4163, 4200, 4262, 4292, 4485, 4513, 4554], '(91)': [343], '3Benya': [346], 'Renal': [348], 'Physiol.': [349], '3:': [351], '30-35PubMed': [352], '4Sage': [355], 'Trueb': [357], 'B.': [358, 409, 1404, 4125, 4150], '1983;': [364], '258:': [365], '13391-13401Abstract': [366], 'Scholar)': [372, 2968, 4295], 'has': [374, 607, 631, 961, 2680, 2936, 3341, 3352, 3673, 4068, 4099, 4296], 'subsequently': [375], 'produced': [380, 3236, 3302, 3786, 4593], 'by': [381, 750, 1386, 1826, 1917, 2059, 2119, 2182, 2290, 2313, 2396, 2825, 3169, 3241, 3270, 3575, 3668, 3787, 3914, 4044, 4168, 4594], 'smooth': [382], 'muscle': [383], 'cells,': [384, 386], 'mast': [385], 'certain': [388], 'tumor': [389], 'cell': [390, 1021, 1054, 2447, 3505, 4469, 4596], 'lines': [391], '(5Macbeath': [392, 898, 4473], 'J.R.E.': [393, 899, 4474], 'Kielty': [394, 900, 4475], 'C.M.': [395, 901, 4476, 4541], 'Shuttleworth': [396, 902, 4477], 'C.A.': [397, 903, 4478], 'Biochem.': [398, 836, 904, 975, 1268, 2097, 2168, 2702, 2959, 3859, 4256, 4284, 4479], '1996;': [400, 906, 979, 2617, 3729, 4196, 4288, 4481, 4550], '319:': [401, 907, 4482], '993-998Crossref': [402, 908, 4483], '(46)': [405, 911, 4486], '6Ruger': [408], 'Dunbar': [410], 'R.': [411, 417, 541, 575, 773, 831, 869, 1257, 2691, 3420, 3515, 4192], 'Hasan': [412], 'Q.': [413], 'Sawada': [414], 'Kittleberger': [416], 'Greenhill': [418, 834, 874, 3520], 'N.': [419, 495, 676, 1828, 3459], 'Neale': [420], 'T.J.': [421], 'Int.': [422], 'Exp.': [424], 'Pathol.': [425], '1994;': [426], '75:': [427], '397-404PubMed': [428], '7Paulus': [431], 'W.': [432, 4117], 'Sage': [433, 544, 926, 4501], 'E.H.': [434, 545], 'Liszka': [435], 'U.': [436, 2643, 3755], 'Iruela-Arispe': [437, 850], 'M.L.': [438, 851], 'Jellinger': [439], 'K.': [440, 560, 573, 579, 759, 771, 777, 2637, 2647, 3405, 3418, 3424, 3749, 3759, 4020, 4075, 4077, 4148, 4182], 'Br.': [441], 'Cancer.': [443], '1991;': [444, 531, 712, 1784, 3862], '63:': [445], '367-371Crossref': [446], '(28)': [449], '8Sage': [452], 'Balian': [454], 'G.': [455], 'Vogel': [456], 'A.M.': [457, 2607, 3719], 'Lab.': [460], 'Invest.': [461], '1984;': [462], '50:': [463], '219-231PubMed': [464], 'Scholar).': [466, 720, 789, 886, 941, 1277, 1792, 2177, 2220, 2355, 2663, 3436, 3532, 3775, 3868, 4035, 4094, 4166, 4203, 4265, 4557], 'distinct': [468], 'identified,': [472], 'α2(VIII).': [475, 1810, 2738], 'Pepsin': [476], 'digestion': [477, 2109, 2811, 2935, 3789], "Descemet's": [479, 723, 3376], 'membrane': [480, 724], 'suggested': [481, 964], 'heterotrimers': [483], 'two': [485, 1424, 1731, 3446, 3804], 'one': [489, 1338, 4587], 'are': [492, 619, 1030, 1806, 2570, 2984, 3685, 4318, 4358, 4371, 4384, 4404, 4412], 'formed': [493, 3996, 4319], '(9Yamaguchi': [494, 675, 3458], 'Benya': [496, 677, 3460], 'van': [498, 679, 3462], 'der': [499, 680, 2645, 3463, 3757], 'Rest': [500, 681, 3464], 'M.': [501, 682, 3465, 4520], 'Ninomiya': [502, 524, 683, 705, 1777, 3466], 'Y.': [503, 517, 525, 684, 698, 706, 1770, 1778, 3467, 4079, 4081], '1989;': [507, 688, 840, 3471], '264:': [508, 689, 3472], '16022-16029Abstract': [509, 690, 3473], '10Muragaki': [516, 697], 'Jacenko': [518, 699, 1771], 'O.': [519, 700, 1772], 'Apte': [520, 701, 1773], 'S.': [521, 702, 1265, 1774, 2609, 2631, 2699, 3721, 3743, 4073, 4524, 4537], 'Mattei': [522, 703, 1775], 'M.G.': [523, 704, 1776], 'Olsen': [526, 707, 1779], 'B.R.': [527, 708, 1780], '266:': [532, 713, 1785], '7721-7727Abstract': [533, 714, 1786], '11Kapoor': [540], '25:': [548], '3930-3937Crossref': [549], '(58)': [552], '12Sawada': [555], 'Konomi': [557, 756, 3402], 'Hirosawa': [559, 758, 3404], 'Cell': [562, 761, 1075, 1077, 3407, 4526], '1990;': [564, 584, 763, 782, 879, 3409, 3429, 3525], '110:': [565, 764, 3410], '219-227Crossref': [566, 765, 3411], '(152)': [569, 768, 3414], '13Mann': [572, 3417], 'Jander': [574, 772, 3419], 'Korsching': [576, 774, 3421], 'E.': [577, 775, 931, 2639, 3422, 3751, 4506], 'Kuhn': [578, 776, 3423], 'Rauterberg': [580, 778, 3425], 'FEBS': [582, 780, 3427], 'Lett.': [583, 781, 3428], '273:': [585, 783, 2652, 3430, 3764], '168-172Crossref': [586, 784, 3431], '(34)': [589, 787, 3434], 'Scholar),': [591, 986, 2106, 3479], 'although': [592, 987, 2907, 3649], 'helix,': [611, 3149], 'similar': [620, 744, 2550, 2902, 3139, 3452], 'α1(X)': [623, 1816, 3324, 3679, 4219], 'X': [627, 1857, 2954, 3440, 3481, 3661, 3826, 4112], 'Sequence': [629], 'analysis': [630, 2312, 3122], 'each': [633, 1418, 1463, 1646, 3074], 'contain': [636, 1343], 'collagenous': [638, 2824], 'domain': [639, 1322, 1746, 1768, 2569, 3008, 3783, 3835], '454': [641], 'amino': [642, 1804, 1962, 3950], 'acids,': [643], 'N-terminal': [646], 'non-triple': [647, 671], 'helical': [648, 672, 2950, 3007, 3265, 3912, 3957], 'region': [649, 673], '(NC2),': [650], '1The': [651], 'abbreviations': [652], 'used': [653, 1415, 1563, 2075], 'are:': [654], 'NC2': [655, 1321, 1745, 2506, 2995], 'NC1non-collagenous': [657], 'domains': [658, 791, 2992, 3113], '2': [659, 792, 1237, 1481, 1725, 2051, 2123, 2132, 2294, 2300, 2477], '1,': [661, 794, 2475, 2521], 'respectivelyPCRpolymerase': [662], 'reactionPAGEpolyacrylamide': [664], 'gel': [665, 800], 'electrophoresis.': [666, 801], 'longer': [669], 'C-terminal': [670], '(NC1)': [674], 'Studies': [721], 'suggest': [725, 4578], 'major': [732, 2450, 2469, 3304, 4570], 'component': [733], 'hexagonal': [736, 3397], 'lattice': [737, 745, 3398], 'seen': [738, 2841, 3059, 3278], 'tissue,': [741], 'structure': [746, 3351, 3399, 3561], 'can': [747, 968, 2745, 3507, 3604, 4302], 'elaborated': [749], 'cultured': [751], '(12Sawada': [754, 3400], 'Scholar,13Mann': [770], 'non-collagenous': [790, 2991, 3112], 'respectively': [795], 'polymerase': [796, 1887, 1893], 'reaction': [798, 1647, 1879, 2179], 'polyacrylamide': [799], 'thought': [806, 2561], 'play': [808], 'key': [810], 'structural': [811], 'role': [812], 'vasculature,': [815], 'where': [816, 2558, 4461], 'it': [817, 891, 2559, 2908, 3496, 4445, 4450, 4462], 'found': [819, 1341, 3326], 'subendothelium': [823], 'elastin-rich': [827], 'tunica': [828], 'media': [829], '(14Kittleberger': [830], 'Davis': [832, 870, 3516], 'P.F.': [833, 871, 3517], 'N.S.': [835, 875, 3521], 'Biophys.': [837, 976, 4085, 4285], 'Res.': [838, 878, 933, 977, 3524, 4286, 4508, 4549], 'Commun.': [839, 978, 4287], '159:': [841], '414-419Crossref': [842], '(26)': [845], '15Sage': [848], 'Structure,': [852], 'Molecular': [853], 'Biology': [854], 'Pathology': [856], 'Collagen.': [858], 'New': [859, 864], 'York': [860], 'Academy': [861], 'Sciences,': [863], 'York1990:': [865], '17-31Google': [866], '16Kittleberger': [868], 'Flynn': [872, 3518], 'D.W.': [873, 3519], 'Connect.': [876, 3522], 'Tissue': [877, 3523], '24:': [880, 3526], '303-318Crossref': [881, 3527], '(47)': [884, 3530], 'Recent': [887], 'evidence': [888, 989, 2784, 4298], 'indicates': [889], 'up-regulated': [893], 'injury': [897], '17Sibinga': [914, 4489], 'N.E.S.': [915, 4490], 'Foster': [916, 4491], 'L.C.': [917, 4492], 'Hsieh': [918, 4493], 'C.H.': [919, 4494], 'Perrella': [920, 4495], 'M.A.': [921, 4496, 4547], 'Lee': [922, 928, 4497, 4503], 'W.S.': [923, 4498], 'Endege': [924, 4499], 'W.O.': [925, 4500], 'M.E.': [929, 3856, 4504], 'Haber': [930, 4505], 'Circ.': [932, 4507, 4548], '1997;': [934, 4129, 4154, 4509], '80:': [935, 4510], '532-540Crossref': [936, 4511], '(78)': [939, 4514], 'Despite': [942, 3334], 'importance': [944, 4060, 4102], 'molecule,': [947], 'little': [948], 'known': [950, 1721, 3343], 'about': [951, 992, 3362], 'or': [954, 1890, 1980, 2325, 2335, 2366, 2471, 2920, 2978, 3190, 4409], 'supramolecular': [955, 3367, 3560], 'organization': [956], 'vasculature.': [959], 'It': [960, 3672], 'recently': [962], 'homotrimers': [970, 3001, 3235, 4304], '(18Rosenblum': [973, 4282], 'N.D': [974, 4283], '227:': [980, 2349, 4289], '205-210Crossref': [981, 4290], '(13)': [984, 4293], 'no': [988, 2468, 4003, 4385], 'presented': [991, 4297], 'stability': [993, 2574, 3146, 3229, 4037, 4067], 'helix.': [996, 1327, 3052, 3982], 'In': [997, 2897, 3776, 4095, 4215, 4266], 'report,': [999], 'describe': [1001], 'expression': [1003], 'constructs': [1006, 2435], 'vitrotranslation': [1014], 'fibroblast': [1020, 2446], 'line,': [1022], 'show': [1024, 3598, 3988], 'capable': [1031], 'forming': [1033], 'Unless': [1036], 'otherwise': [1037], 'stated,': [1038], 'all': [1039, 1052, 3540, 3960, 4402], 'chemicals': [1040], 'were': [1041, 1057, 1068, 1091, 1111, 1137, 1148, 1171, 1192, 1212, 1243, 1330, 1439, 1494, 1562, 1648, 1734, 1795, 1837, 1861, 1940, 2001, 2026, 2039, 2057, 2074, 2086, 2147, 2201, 2240, 2270, 2297, 2316, 2357, 2373, 2394, 2532, 2543, 2669, 2729, 2791, 2804, 2812, 2820, 2840, 3018, 3252, 3268, 3622, 3628, 3642, 3907, 4006], 'obtained': [1042, 1058, 1069, 1092, 1112, 1149, 1172, 1181, 1193, 1204, 4007], 'BDH/Merck': [1044], '(Poole,': [1045, 1141], 'Dorset,': [1046, 1142], 'United': [1047], 'Kingdom).': [1048], 'medium': [1050, 1218], 'other': [1053, 3894], 'culture': [1055], 'reagents': [1056], 'Life': [1060], 'Technologies,': [1061], 'Ltd.': [1062, 1185], '(Paisley,': [1063], 'Renfrewshire,': [1064], 'Scotland).': [1065], 'HT-1080': [1066, 1210, 1241, 1999, 4240], 'fibroblasts': [1067], 'European': [1072], 'Collection': [1073], 'Cultures': [1076], 'Bank.': [1078], 'Restriction': [1079], 'enzymes,': [1080], 'nuclease': [1081], 'S7,': [1082], 'RNasin,': [1083, 1902, 1978], 'Expand': [1086, 1444], 'High': [1087, 1200, 1264, 1445, 2698], 'Fidelity': [1088, 1446], 'PCR': [1089, 1389, 1413, 1447, 1643, 1897, 2762], 'kit': [1090, 1159, 1448, 1489, 1912], 'Boehringer': [1094], '(Lewes,': [1095], 'Sussex,': [1096, 1154], 'UK).': [1097, 1116, 1143, 1155, 1177, 1199, 1209], 'Flexi®': [1099], 'reticulocyte': [1100, 1946], 'lysate': [1101, 1947], 'kit,': [1103, 1108], 'transcription': [1107, 1911], 'nucleotides': [1110], 'Promega': [1114], '(Southampton,': [1115], 'Protein': [1117], 'A-Sepharose': [1118], 'beads,': [1119], 'phenylmethylsulfonyl': [1120], 'fluoride,': [1121], 'N′-ethylmaleimide,': [1122], 'chymotrypsin,': [1123], 'proteinase': [1124, 2206], 'K,': [1125], 'trypsin,': [1126], 'high': [1127, 2156, 2516, 2665, 2829, 3809, 4050], 'purity': [1128, 1201, 2157, 2830], 'collagenase': [1129, 2159, 2832, 3788], 'III,': [1131], 'soybean': [1132, 2126], 'trypsin': [1133, 2127], 'inhibitor,': [1134], 'α,α′-dipyridyl': [1136, 2034], 'purchased': [1138, 1161], 'Sigma': [1140], 'RNA': [1144, 1886, 1892, 1914, 1985, 2425, 2461], 'purification': [1145], 'spin': [1146, 1923], 'columns': [1147], 'QIAGEN': [1151], 'Inc.': [1152], '(Crawley,': [1153], 'TA': [1157, 1690], 'Cloning®': [1158, 1691], 'Invitrogen': [1163], '(Leek,': [1164], 'Netherlands).': [1166], 'Prestained': [1167], 'molecular': [1168, 2389, 2488, 2517, 2666, 2912, 3638, 4565], 'mass': [1169, 2489, 2518, 2667, 2913, 3639], 'markers': [1170], 'R&D': [1174], 'Systems': [1175], '(Oxon,': [1176], 'Tran35S': [1178], 'label': [1179, 2752, 3107], 'ICN': [1183], 'Biomedicals': [1184], '(Thame,': [1186], 'Oxon,': [1187], 'UK),': [1188], '14C-methylated': [1190], 'proteins': [1191, 2399], 'Amersham': [1195], 'Pharmacia': [1196], 'Biotech': [1197], '(Buckinghamshire,': [1198], 'digitonin': [1202, 1251], 'Calbiochem': [1206], '(Beeston,': [1207], 'Nottingham,': [1208], 'maintained': [1213, 1711], "Dulbecco's": [1215], 'modified': [1216], "Eagle's": [1217], '(containing': [1219], 'sodium': [1220, 1968], 'pyruvate': [1221], '(0.11': [1222], 'g/liter)': [1223], 'pyridoxine)': [1225], '10%': [1228, 2336], '(v/v)': [1229], 'fetal': [1230], 'calf': [1231], 'serum,': [1232], '100': [1233, 2361, 2546, 2877, 3655], 'μg/ml': [1234, 1967, 2205], 'streptomycin/penicillin,': [1235], 'mml-glutamine.': [1238], 'Trypsinized': [1239], 'confluent': [1240], 'washed': [1244], 'phosphate-buffered': [1246], 'saline': [1247], 'permeabilized': [1249], '(40': [1252], 'μg/μl)': [1253], 'described': [1255, 2092, 2163, 2209, 2309, 3596], '(19Wilson': [1256, 2690], 'Allen': [1258, 2692], 'A.J.': [1259, 2693], 'Oliver': [1260, 2694], 'Brookman': [1262, 2696], 'J.L.': [1263, 2697], 'Bulleid': [1266, 2095, 2166, 2700, 2957, 4254], 'N.J.': [1267, 2096, 2167, 2211, 2701, 2958, 4255], '1995;': [1270, 2589, 2704, 3701], '307:': [1271, 2705], '679-687Crossref': [1272, 2706], '(133)': [1275, 2709], 'A': [1278, 1681, 1811, 3178, 3315, 4279], 'clone': [1280, 1339, 1378, 1394, 1813], 'previously': [1284, 2937], 'isolated': [1285, 2058, 2081, 2142, 2196, 2235, 2802, 4239], 'λgt11': [1288, 1333, 1367], 'human': [1289, 1809, 4355], 'aorta': [1290], 'cDNA': [1291], 'library.': [1292], 'Briefly,': [1293], 'primary': [1294], 'screening': [1295, 1311], 'carried': [1297, 1440, 1862, 1941, 2028, 2111, 3171, 3181, 3320], 'out': [1298, 1441, 1863, 1942, 2029, 2112, 3172, 3182, 3321], 'probes': [1300], 'designed': [1301, 1317, 1495], 'NC1': [1304, 1727, 1767, 2568, 2850, 2895, 2932, 2993, 3670, 3683, 3782, 3834, 3887], 'domain.': [1305, 2507, 3671], 'Positive': [1306], 'clones': [1307, 1831, 1870], 'underwent': [1308], 'secondary': [1310], 'using': [1312, 1332, 1449, 1588, 1943], '496-base': [1314], 'pair': [1315, 1677, 1683], 'probe': [1316], 'according': [1318], 'part': [1324], 'Subsequent': [1328, 1658], 'positives': [1329], 'sequenced': [1331], 'forward': [1334, 1508, 1591, 1664], 'reverse': [1336, 1520, 1598, 1625, 1670], 'primers;': [1337], 'base': [1345, 1358, 1571, 1732], 'pairs': [1346, 1359, 1579], '3′-untranslated': [1348], 'region,': [1349], 'entire': [1351, 1381], 'coding': [1352, 1382], 'sequence': [1353, 1383, 1497, 1585, 1620, 1728], 'α1(VIII),': [1355], '∼200': [1357], '5′-untranslated': [1361], 'region.': [1362], 'This': [1363, 2548, 2739, 3087, 3100, 3533], 'excised': [1365], 'subcloned': [1369], 'into': [1370, 1689, 3615, 3795], 'EcoRI-cut': [1371], 'pBluescript(+)': [1372], 'designated': [1374, 1697], 'pBlu-HaI8.': [1375], 'An': [1376, 1573], 'containing': [1379, 1395, 1881, 1931, 1955, 2190], 'generated': [1385, 1587], 'overlap': [1387, 1678], 'extension': [1388, 1679], 'mouse': [1392, 1722, 4392, 4419], 'genomic': [1393], 'exons': [1396], '3': [1397, 1492, 1528, 1597, 1609, 1663, 1724, 1906, 2364, 2523, 2798], '4,': [1399, 3199], 'pNF-m129-21': [1400, 1501], '(gift': [1401], 'Prof.': [1403], 'Olsen,': [1405], 'Boston,': [1406], 'MA).': [1407], 'First': [1408], 'round': [1409, 1429], 'proofreading': [1410], 'hot': [1411], 'start': [1412, 1515, 3954], 'generate': [1417, 1565], 'exon': [1419, 1527, 1549, 1559, 1581, 1596, 1608, 1618, 1632, 1662, 1668, 1703, 1723], 'separately.': [1420], 'Combination': [1421], 'products': [1425, 1644, 2393, 2451, 2470, 2519, 2668, 2734, 2771, 2819, 3017, 3186, 3248], 'second': [1428, 3932], 'allowed': [1430, 1651, 1672, 4209], 'fusion': [1431, 1704], 'elongation': [1433], 'product.': [1437, 1680], 'Reactions': [1438], "manufacturer's": [1451], 'recommended': [1452], 'cycling': [1453, 2275], 'Reaction': [1455, 1949], 'mixtures': [1456, 1950, 2038, 2085, 2090, 2146, 2200, 2239], '(50': [1457, 2129], 'μl)': [1458, 1954], 'comprised': [1459], '30': [1460, 2007, 2416, 3226], 'pmol': [1461], 'primer,': [1464], '∼10': [1465], 'ng': [1466], 'template': [1468], 'DNA,': [1469], '2.6': [1470], 'units': [1471, 1883, 1900, 1936, 1976], 'enzyme': [1473], 'mixture': [1474, 1880, 3919], '(thermostable': [1475], 'Taq': [1476], 'Pwo': [1478], 'DNA': [1479], 'polymerases),': [1480], 'mm': [1482, 1904, 1907, 2020, 2048, 2192, 2251, 2254], 'MgCl2,': [1483], '200': [1484], 'nm': [1485, 1993], 'dNTPs': [1486], '1×': [1488, 1910], 'buffer.': [1490, 1913, 2139, 2232], 'Exon': [1491], 'primers': [1493], 'supplied': [1498], 'clone.': [1502], '2N.': [1503], 'Fukai,': [1504], 'personal': [1505], 'communication.': [1506], 'primer': [1509, 1521, 1592, 1599, 1626, 1665, 1671], '(5′-CGAATTCGCCATGCAGGGGGCTCTG)': [1510], 'included': [1511], 'initial': [1513], 'ATG': [1514], 'site': [1516, 3978], '(boldface),': [1517], '(5′-CGGCAGCAGCGGTAGAGGCATTTCCAAGTACTGGCCTTTGC)': [1522], 'represented': [1523], 'chimera': [1525], '4': [1530, 1550, 1582, 1619, 1633, 1669, 1996, 2150, 2891, 3201], 'sequence,': [1532, 4393], 'first': [1535, 1603, 4312], '23': [1536], 'bases': [1537, 1605], 'based': [1538, 1606, 1614, 2971], 'on': [1539, 1607, 1615, 1864, 2023, 2264, 2332, 2972, 3159, 3183, 3322, 3819, 3828, 4390, 4608], 'positions': [1540, 4115], '51': [1541], '29': [1543], 'published': [1546, 1617], '5′-end': [1547], '(italics)': [1551, 1610], 'rest': [1554], '3′-end': [1557, 1630], '3.': [1560], 'These': [1561, 1793, 2664, 3632], 'expected': [1567, 1574], 'fragment': [1568, 1684, 3197, 3936], '215': [1570], 'pairs.': [1572], '1.9': [1577], 'kilobase': [1578], 'representing': [1580], 'chimeric': [1590], 'complementary': [1593], '(5′-CCGAGAGGGCAAAGGCCAGTACTTGGAAATGCCTCTACCGCTGC),': [1600], '26': [1604], 'remainder': [1613], '(positions': [1621, 1634], '29–46),': [1622], '(5′-GGCTCCGAATTATGTGGGGCAGAGC)': [1627], '1923–1938)': [1635], 'including': [1636], 'stop': [1639], 'codon': [1640], '(boldface).': [1641], 'Purified': [1642], 'combined': [1649], 'self-prime': [1653], 'six': [1655], 'thermal': [1656, 2274], 'cycles.': [1657], 'addition': [1659, 2121, 2184], 'amplification': [1673], '2.1-kilobase': [1676, 1682], 'purified': [1686, 1916], 'cloned': [1688], 'vector': [1692, 1819, 1841], 'pCR': [1693, 1698, 1833], '2.1': [1694], '2.1-MFa28.': [1699], 'Sequencing': [1700], 'showed': [1701, 1729, 2783, 3129], 'successful': [1702], 'correct': [1707, 3625], 'reading': [1708], 'frame': [1709], 'being': [1710], 'across': [1712], 'boundary': [1714], '(data': [1715, 3331], 'not': [1716, 1796, 2670, 2717, 3193, 3332, 4401, 4443], 'shown).': [1717, 3333], 'Comparison': [1718], 'changes': [1733, 1794], 'present,': [1735], 'resulting': [1736, 2879], 'methionine': [1739, 3106], 'leucine': [1741], 'change': [1742, 1761], '(position': [1747], '66)': [1748], 'downstream': [1749], 'predicted': [1752, 2487], 'signal': [1753], 'peptide': [1754], 'cleavage': [1755, 3977], 'serine': [1758], 'threonine': [1760, 4170], 'at': [1762, 1874, 2006, 2064, 2359, 2534, 2545, 2843, 2856, 2863, 2876, 2919, 3225, 3279, 3290, 3577, 3646, 4388], 'position': [1763], '239': [1764], '(10Muragaki': [1769], 'considered': [1797], 'problem': [1801], 'because': [1802], 'acids': [1805, 1963, 3951], 'present': [1807, 2756, 2792, 4361, 4389, 4576], 'pGEM-3Z': [1820], '(designated': [1821], 'pGEM-BX)': [1822], 'kindly': [1824], 'donated': [1825], 'Dr.': [1827, 4600], 'Bulleid.': [1829], 'pBlu-HaI8,': [1832], '2.1-MFa28,': [1834], 'pGEM-BX': [1836, 1895], 'linearized': [1838, 1869], 'unique': [1840], 'restriction': [1842], 'sites': [1843], '3′': [1844], 'inserts:': [1847], 'HindIII': [1848], '(for': [1849, 1888, 1894], 'clones)': [1853], 'SalI': [1855], '(type': [1856], 'clone).': [1859], 'Transcriptions': [1860], '10': [1865, 1903, 2243, 2253, 2267, 2370], 'μg': [1866], '3–4': [1872], 'h': [1873, 2005, 2151], '37': [1875], '°C': [1876, 2292, 2362, 2368, 2536, 2865, 3281, 3292, 3330, 4276, 4332], '>100': [1882], 'T3': [1885], 'pBlu-HaI8)': [1889], 'T7': [1891], '2.1-MFa28),': [1898], '∼70': [1899], 'dithiothreitol,': [1905], 'NTPs,': [1908], 'binding': [1918], 'commercially': [1921], 'available': [1922, 3371, 4386], 'column': [1924], 'eluted': [1926], 'diethyl': [1928], 'pyrocarbonate-treated': [1929], 'water': [1930, 2466], '1': [1932, 2153, 2225, 2291], 'mmdithiothreitol': [1933], '∼35': [1935], 'RNasin.': [1938], 'Translations': [1939, 2025], 'system.': [1948, 3593, 4613], '(volume': [1951], '51.5': [1953], '35': [1956], 'μl': [1957, 1982, 2124, 2244], 'lysate,': [1959], '20': [1960, 2047], 'μm': [1961], '(minus': [1964], 'methionine),': [1965], '50': [1966, 1992, 2246, 2287], 'ascorbate,': [1969], '25': [1970, 2019], 'μCi': [1971], '[35S]': [1973], 'methionine,': [1974], '∼140': [1975], '2.5': [1979], '5': [1981, 2069, 2114, 2191, 2527, 3078, 3285, 3296], 'transcribed': [1984], '(substituted': [1986], 'sterile': [1988, 2465], 'H2O': [1989], 'controls),': [1991], 'KCl,': [1994], '×': [1997, 2066], '105permeabilized': [1998], 'translated': [2002, 2392, 3607, 3620], '1.5': [2004], 'After': [2009], 'time,': [2011], 'N-ethylmaleimide': [2012], 'added': [2014, 2271], 'concentration': [2017], 'stored': [2022], 'ice.': [2024], 'also': [2027, 3089], 'presence': [2032, 2539, 3175, 3929, 3944, 4012], '(89': [2035], 'μg/ml).': [2036], 'Translation': [2037, 2423, 3247], 'diluted': [2040], 'five': [2041], 'times': [2042], '0.1': [2044], 'mpotassium': [2045], 'acetate,': [2046, 2053], 'HEPES,': [2049], 'mmmagnesium': [2052], 'pH': [2054, 2248, 2256], '7.2;': [2055], 'centrifugation': [2060], 'microcentrifuge': [2063], '14,000': [2065], 'g': [2067], 'min;': [2070], 'appropriate': [2072, 4107], 'aliquots': [2073], 'subsequent': [2077], 'investigations.': [2078], 'Aliquots': [2079, 2140, 2194, 2233], 'treated': [2087, 2805], 'chymotrypsin/trypsin': [2089, 2306, 3179, 3262, 3918], '(20Middleton': [2093, 2164, 2955, 4252], 'R.B.': [2094, 2165, 2956, 4253], '1993;': [2099, 2170, 2961, 4258, 4528], '296:': [2100, 2171, 2962, 4259], '511-517Crossref': [2101, 2172, 2963, 4260], '(21)': [2104, 2175, 2966, 4263], 'except': [2107], 'min.': [2115, 2268, 2295, 2371], 'Digestion': [2116, 2221], 'stopped': [2118, 2223], 'inhibitor': [2128, 4018], 'mg/ml)': [2130], 'volumes': [2133], 'boiling': [2135], '2×': [2136, 2186, 2229, 2320], 'SDS-PAGE': [2137, 2187, 2230, 2321], 'loading': [2138, 2188, 2231, 2322], 'digested': [2148, 2202, 3019, 3127, 3260], 'unit': [2154], 'bacterial': [2158, 2831], 'III': [2161, 2834], 'terminated': [2181], 'buffer': [2189], 'EDTA.': [2193], '250': [2204], 'K': [2207, 2765], '(21Bulleid': [2210], 'Nature.': [2212, 2347], '1988;': [2213], '335:': [2214], '649-651Crossref': [2215], '(261)': [2218], 'mphenylmethylsulfonyl': [2226], 'fluoride': [2227], 'resuspended': [2241], 'mmTris,': [2247], '7.5,': [2249], '150': [2250], 'NaCl,': [2252], 'EDTA,': [2255], '8.0,': [2257], '1%': [2259], 'Triton': [2260, 2807], 'X-100': [2261, 2808], 'left': [2263], 'ice': [2265], 'Samples': [2269, 2296, 2315, 2356], 'block': [2276], 'subjected': [2278, 2303, 3253], 'range': [2281], 'temperatures': [2283, 3258], '36': [2285], 'increasing': [2289], 'every': [2293, 2299], 'removed': [2298], 'min': [2301, 2365], 'digest': [2307, 3180], 'above': [2310], 'before': [2311], 'SDS-PAGE.': [2314], 'taken': [2317], 'up': [2318, 4610], 'buffer,': [2323], 'without': [2326], '0.7': [2327], 'm': [2328], 'β-mercaptoethanol,': [2329], 'analyzed': [2331], 'either': [2333, 2360, 2725, 3188], '8': [2334], '(digested': [2337], 'samples)': [2338], 'gels': [2339], 'following': [2340], 'method': [2342], 'Laemmli': [2344], '(22Laemmli': [2345], 'U.K.': [2346], '1970;': [2348], '680-685Crossref': [2350], '(207231)': [2353], 'denatured': [2358], '60': [2367, 2535, 2864, 3647], 'Gels': [2372], 'fixed,': [2374], 'vacuum-dried': [2375], 'onto': [2376], 'filter': [2377], 'paper,': [2378], 'placed': [2380], 'against': [2381], 'Kodak': [2382], 'X-Omat': [2383], 'AR': [2384], 'film': [2385], 'analysis.': [2387], 'Relative': [2388], 'masses': [2390], 'determined': [2395, 4043], 'reference': [2397], 'to14C-methylated': [2398], '(myosin,': [2400], '220': [2401], 'kDa;': [2402, 2405, 2410, 2413, 2417], 'phosphorylaseb,': [2403], '97.4': [2404], 'serum': [2407], 'albumin,': [2408], '66': [2409], 'ovalbumin,': [2411], '46': [2412], 'carbonic': [2414], 'anhydrase,': [2415], 'lysozyme,': [2419], '14.3': [2420], 'kDa)': [2421], '(Calbiochem).': [2422], 'derived': [2426], 'recombinant': [2430], 'line': [2448], '(Fig.': [2455, 2474, 2520, 2795, 2835, 2866, 2925, 3076, 3198, 3284, 3295, 3799], '1).': [2456], 'Control': [2457], 'reactions': [2458], 'which': [2460, 2531, 3390, 4227, 4348], 'substituted': [2463], 'yielded': [2467], 'background': [2472], 'bands': [2473, 2482, 2790, 2839, 3066, 3128], 'lanes': [2476, 2522, 2526, 2797, 2868, 3028, 3082, 3200, 3204], 'and8).': [2478], '80-kDa': [2481, 2789], 'corresponded': [2483], 'approximately': [2484], 'single': [2491], 'monomeric': [2492, 2814], '(73': [2494], 'kDa).': [2495], 'As': [2496], 'expected,': [2497], 'ran': [2499, 2918], 'slightly': [2500, 3311, 3933], 'faster': [2501], 'due': [2502, 3941], 'shorter': [2505], 'associated': [2513, 4456], '9and': [2525], '11,': [2529, 2893], 'respectively),': [2530], 'SDS,': [2541], 'susceptible': [2544, 2671, 3629], 'results': [2553, 3987, 4396, 4577], 'observed': [2554, 2730, 3090], 'X,': [2557], 'strong': [2563, 3875], 'noncovalent': [2564, 3876], 'interactions': [2565, 3877], 'within': [2566, 3813, 3832], 'responsible': [2571, 3872], 'oligomer': [2573], 'under': [2575, 3687], 'denaturing': [2576, 3688], 'conditions': [2577, 3689], '(23Chan': [2578, 3690], 'D.': [2579, 2603, 3691, 3715, 4601], 'Cole': [2580, 3692], 'W.G.': [2581, 3693], 'Rogers': [2582, 3694], 'J.G.': [2583, 3695], 'Bateman': [2584, 2612, 3696, 3724], 'J.F.': [2585, 2613, 3697, 3725], '270:': [2590, 3702], '4558-4562Abstract': [2591, 3703], '(69)': [2599, 3711], '24Chan': [2602, 3714], 'Weng': [2604, 3716], 'Y.M.': [2605, 3717], 'Hocking': [2606, 3718], 'Golub': [2608, 3720], 'McQuillan': [2610, 3722], 'D.J.': [2611, 3723, 4022, 4083], '271:': [2618, 3730], '13566-13572Abstract': [2619, 3731], '(45)': [2627, 3739], '25Frischholz': [2630, 3742], 'Beier': [2632, 3744], 'F.': [2633, 3745, 4190], 'Girkontaite': [2634, 3746], 'I.': [2635, 3747], 'Wagner': [2636, 3748], 'Poschl': [2638, 3750], 'Turnay': [2640, 3752], 'Mayer': [2642, 3754], 'von': [2644, 3756], 'Mark': [2646, 3758], '1998;': [2651, 3763], '4547-4555Abstract': [2653, 3765], '(55)': [2661, 3773], 'reducing': [2673], 'use': [2676, 4205], 'allow': [2684], 'formation': [2686, 2946, 3218], 'disulfide': [2688, 2714], 'bonds': [2689, 2715, 4046], 'Scholar);': [2711], 'however,': [2712], 'interchain': [2713], 'do': [2716], 'appear': [2718, 4397], 'involved': [2721], 'oligomerization': [2723], 'chain.': [2726], 'Differing': [2727], 'intensities': [2728, 3267], 'between': [2731, 3060, 3444, 4047], 'reproducible': [2742], 'finding': [2743, 4352], 'attributed': [2747], 'differing': [2749, 3054], 'amounts': [2750, 3069], 'incorporation': [2753], '(22': [2754], 'methionines': [2755], '7': [2760, 3205], '2.1-MFa28).': [2763], 'Proteinase': [2764], 'digests': [2766], 'confirmed': [2767], 'translocation': [2768], 'protein': [2770, 3364], 'cellular': [2774], 'rough': [2775], 'endoplasmic': [2776, 4250], 'reticulum': [2777, 4251], '(Fig.2).': [2778], 'compartmental': [2786], 'protection.': [2787], 'Intact': [2788], 'after': [2793, 4008], 'treatment': [2794], '2,': [2796], 'and9).': [2799], 'Only': [2800, 2989], 'when': [2801, 3652], 'prior': [2809], 'degraded.': [2816], 'their': [2826, 3915], 'susceptibility': [2827], '3).': [2836], 'collagenase-resistant': [2838, 2903, 3682, 3886], 'migrating': [2842], '∼22': [2844], '(assumed': [2846], 'represent': [2848, 3966], 'domain),': [2851], 'diffuse': [2854], 'band': [2855, 2889, 2904, 3024, 3055, 3266], '∼50': [2857], 'kDa.': [2858], 'latter': [2860, 3301], '3,': [2867, 3800], '3and': [2869], '10,': [2870, 3030], 'NC1x': [2871, 3801], 'arrow),': [2872], 'eliminated': [2875], 'corresponding': [2882], 'increase': [2883], 'intensity': [2885, 3056], '22-kDa': [2888], '(lanes': [2890], 'α1': [2894], 'arrow).': [2896, 2933, 3802], 'case': [2899, 4268], 'observed,': [2906], 'had': [2909, 4221], 'lower': [2911], '∼18': [2915], 'near': [2921], 'dye': [2923], 'front': [2924], '3,lanes': [2926], '6,': [2927], '7,': [2928], '13,': [2929], 'and14,': [2930], 'α2': [2931], 'Chymotrypsin/trypsin': [2934], 'demonstrated': [2939], 'reliable': [2942], 'assay': [2943], 'molecules': [2951, 3913, 4580], 'premise': [2974], 'incompletely': [2976], 'folded': [2977, 2980, 2998], 'incorrectly': [2979], 'sensitive': [2985], 'proteolytic': [2987, 3155], 'cleavage.': [2988], 'correctly': [2997, 3220], 'will': [3002, 4567], 'degraded,': [3004], 'leaving': [3005], 'intact.': [3009], 'When': [3010], 'chymotrypsin/trypsin,': [3021], '60-kDa': [3023, 3065, 3196, 3925], 'evident': [3026], '(Fig.4,': [3027], '3,6,': [3029], '14,': [3032, 3085], '60kDa': [3033], 'arrow)': [3034], 'reduced': [3038], 'nonreduced': [3040], 'samples,': [3041, 3094], 'Considerable': [3053], '80-': [3063], 'despite': [3067], 'equal': [3068], 'lane': [3075, 3287, 3298], '4,lanes': [3077], '6': [3080], '13': [3083], 'respectively).': [3086], 'albeit': [3095], 'lesser': [3098], 'extent.': [3099], 'principally': [3101], 'reflects': [3102], 'reduction': [3104], 'upon': [3108], 'removal': [3109], '(45%': [3114], '87.5%': [3118], 'α2(VIII)).': [3120], 'Phosphoimage': [3121], 'undigested': [3125], 'exhibited': [3137], 'efficiency': [3140], 'helix': [3143, 3993, 4041, 4066, 4408], 'therefore': [3151], 'its': [3152, 3366], 'resistance': [3153, 3916], 'attack,': [3156], 'dependent': [3158], 'degree': [3161, 3810], 'hydroxylation': [3163, 3210, 3998, 4062, 4322], 'translations': [3170], 'α,α′-dipyridyl.': [3177], 'did': [3192], 'and12': [3202], '15,respectively),': [3207], 'procollagen': [3212], 'necessary': [3215], 'aligned': [3221], 'assessed': [3240], 'measuring': [3242], 'denaturation': [3244, 3645], '(Tm).': [3246], 'variety': [3256, 3503], '(Fig.5).': [3263], 'Resistant': [3264], 'quantified': [3269], 'phosphoimage': [3271], 'analysis,': [3272], '50%': [3275], 'loss': [3276], 'A,': [3286], '6)': [3288], 'B,': [3297], '9).': [3299], 'smaller': [3312, 3934], 'minor': [3313], 'fragment.': [3314, 3927], 'control': [3316], 'Tm': [3317, 4223, 4273, 4329], 'determination': [3318], '44': [3329, 4225], 'fact': [3336], 'over': [3345], '16': [3346], 'years': [3347], 'gene': [3350, 3453], 'delineated,': [3354], 'still': [3357], 'dearth': [3359], 'information': [3361, 3372], 'structure.': [3368], 'best': [3370], 'membrane,': [3377], 'heterotrimer': [3383], '(α1(VIII))2(α2(VIII)),': [3389], 'appears': [3391, 3483, 4171], 'aggregate': [3393, 3794, 3888], 'Similarity': [3437], 'supports': [3442], 'comparisons': [3443], 'these': [3445, 3553, 3612, 4395, 4430], 'molecules,': [3447], 'they': [3450, 3650], 'structures': [3454], 'hypertrophic': [3490], 'chondrocyte': [3491], 'epiphysial': [3493], 'cartilage,': [3494], 'becoming': [3498, 4447], 'increasingly': [3499], 'clear': [3500, 4448], 'types': [3506, 3806], 'synthesize': [3508], 'secrete': [3510], '(16Kittleberger': [3514], 'raises': [3534], 'question': [3536, 3571], 'whether': [3539], 'same': [3548], 'how': [3552], 'variations': [3554], 'affect': [3558], 'function.': [3563, 4574], 'We': [3564, 4598], 'trying': [3567], 'looking': [3576], 'α-chains': [3582], 'experiments': [3595], 'clearly': [3597, 4070], 'successfully': [3606], 'associate': [3614], 'homotrimeric': [3617], 'molecules.': [3618], 'size': [3626], 'collagenase.': [3631], 'appeared': [3634], 'higher': [3637, 4229], 'aggregates': [3640, 3684], 'disappeared': [3651], 'heated': [3653], 'By': [3657], 'analogy': [3658], 'collagen,': [3662], 'association': [3664], 'presumably': [3666], 'mediated': [3667], 'oligomers': [3680], 'support': [3777], 'this,': [3779], 'presumed': [3781], '50-kDa': [3797], 'complex': [3798], 'share': [3807], 'homology': [3812, 3870], 'domain,': [3815], 'current': [3817], 'thinking': [3818], 'folding': [3820], 'centers': [3827, 3839], 'common': [3829], 'hydrophobic': [3830], 'groupings': [3831], 'acting': [3836], 'nucleation': [3838], 'zipper-like': [3841], 'trimerization': [3842], 'carboxyl-': [3845], 'amino-terminal': [3847], 'direction': [3848], '(26Brass': [3849], 'A.': [3850, 4121, 4146], 'Kadler': [3851], 'K.E.': [3852], 'Thomas': [3853], 'J.T.': [3854], 'Grant': [3855], 'Boot-Handford': [3857], 'R.P.': [3858], 'Soc.': [3860], 'Trans.': [3861], '365Crossref': [3864], '(8)': [3866], 'Such': [3869], 'maybe': [3871], 'here.': [3879], 'However,': [3880], 'absence': [3882], 'implies': [3889], 'requirements': [3895], 'oligomerization.': [3900], 'appearance': [3922], 'proteinase-resistant': [3926, 4004], 'Gly-X-Y': [3947, 4367], 'imperfection': [3948, 3972], '41': [3949, 4244], 'domain;': [3958], 'unlike': [3959], 'imperfections': [3964], 'Gly-X-Gly,': [3967], 'Gly-X-X-X-X': [3971], 'constitute': [3975], 'Unlike': [3983], 'previous': [3984, 4280], 'reports,': [3985], 'our': [3986], 'only': [3995, 4175], 'if': [3997], 'occurs,': [4001], 'fragments': [4005], 'incubation': [4009], 'α,α′-dipyridyl,': [4014], 'prolyl': [4016, 4213], 'hydroxylase': [4017], '(27Juva': [4019], 'Prockop': [4021, 4082], 'Cooper': [4023], 'G.W.': [4024], 'Lash': [4025], 'J.W.': [4026], 'Science.': [4027], '1966;': [4028], '152:': [4029], '92-94Crossref': [4030], '(98)': [4033], 'hydrogen': [4045], 'chains,': [4048], 'imino': [4051], 'acid': [4052], 'content,': [4053], 'possibly': [4055], 'side': [4056], 'interactions.': [4058], '(28Sakakibara': [4072], 'Inouye': [4074], 'Shudo': [4076], 'Kishida': [4078], 'Kobayashi': [4080], 'Biochim.': [4084], 'Acta.': [4086], '1973;': [4087], '303:': [4088], '198-202Crossref': [4089], '(271)': [4092], 'addition,': [4096], 'recent': [4097], 'work': [4098, 4425], 'highlighted': [4100], 'Gly-Pro-Arg': [4104, 4379], 'triplets': [4105, 4380], 'charged': [4108], 'Y': [4114], '(29Yang': [4116], 'Chan': [4118], 'V.C.': [4119, 4142], 'Kirkpatrick': [4120, 4145], 'Ramshaw': [4122, 4143], 'J.A.M.': [4123, 4144], 'Brodsky': [4124, 4149], '272:': [4130, 4155], '28837-28840Abstract': [4131], '(97)': [4139], 'Scholar,30Chan': [4141], 'Beck': [4147], '31441-31446Abstract': [4156], '(80)': [4164], 'Stabilization': [4167], 'glycosylated': [4169], 'significant': [4174], 'vent': [4177], 'worm': [4178], 'cuticle': [4179], '(31Mann': [4181], 'Mechling': [4183], 'D.E.': [4184], 'Bachinger': [4185], 'H.P.': [4186], 'Eckerskorn': [4187], 'C.': [4188, 4545], 'Gaill': [4189], 'Timpl': [4191], 'Mol.': [4194], '255-266Crossref': [4198], '(60)': [4201], 'maximum': [4210], 'modification': [4211], 'residues.': [4214], 'system,': [4217], 'homopolymers': [4220, 4338], 'than': [4230, 4341, 4369, 4420], 'reported': [4232, 4333], 'microsomes': [4241], '(Tm': [4242], '=': [4243], '°C),': [4245], 'alternative': [4247], 'source': [4248], 'homopolymers,': [4271], 'found.': [4278], 'report': [4281], 'cell-free': [4307], 'system;': [4308], 'conclusive': [4313], 'proof': [4314], 'trimers': [4317], 'prerequisite.': [4327], 'here': [4334], 'less': [4340, 4413], 'α1(X),': [4347], 'surprising': [4351], 'since': [4353], 'more': [4359, 4378], 'prolines': [4360, 4403], 'Yposition': [4364], 'triplet': [4368], '(76': [4374], 'versus': [4375], '73)': [4376], '(11versus': [4381], '6).': [4382], 'There': [4383], 'data': [4387], 'indicate': [4399], 'hydroxylated': [4405], 'potential': [4414], 'substrates': [4416], 'human.': [4423], 'Further': [4424], 'required': [4427], 'clarify': [4429], 'apparent': [4431], 'differences': [4432], 'stability.': [4434], 'Although': [4435], 'function': [4437], 'known,': [4444], 'many': [4454], 'situations': [4455], 'inflammation': [4458], 'repair,': [4460], 'provisional': [4466, 4591], 'matrix': [4467], 'attachment': [4470], 'migration': [4472], '32Rooney': [4517], 'Wang': [4519], 'Kumar': [4521, 4523], 'Sci.': [4527], '105:': [4529], '213-218Crossref': [4530], '33Bendeck': [4534], 'M.P.': [4535], 'Regenass': [4536], 'Tom': [4538], 'W.D.': [4539], 'Giachelli': [4540], 'Schartz': [4542], 'S.M.': [4543], 'Hart': [4544], 'Reidy': [4546], '79:': [4551], '524-531Crossref': [4552], '(57)': [4555], 'Clearly,': [4558], 'strong,': [4563], 'assemblies': [4566, 4584], 'determinant': [4571], 'way': [4588], 'modulating': [4590], 'matrices': [4592], 'populations.': [4597], 'thank': [4599], 'John': [4602], 'fellow': [4604], 'workers': [4605], 'advice': [4607], 'setting': [4609]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1559514166', 'counts_by_year': [{'year': 2024, 'cited_by_count': 2}, {'year': 2022, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-15T19:30:23.844682', 'created_date': '2016-06-24'}