Title: Real-time enzymatic degradation of human dentin collagen fibrils exposed to exogenous collagenase: an AFM study in situ
Abstract: Journal of MicroscopyVolume 241, Issue 2 p. 162-170 Real-time enzymatic degradation of human dentin collagen fibrils exposed to exogenous collagenase: an AFM study in situ X. ZHENG, X. ZHENG Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorH. PAN, H. PAN Department of Chemistry, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorZ. WANG, Z. WANG Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorH. CHEN, H. CHEN Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this author X. ZHENG, X. ZHENG Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorH. PAN, H. PAN Department of Chemistry, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorZ. WANG, Z. WANG Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this authorH. CHEN, H. CHEN Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, People's Republic of ChinaSearch for more papers by this author First published: 11 January 2011 https://doi.org/10.1111/j.1365-2818.2010.03412.xCitations: 12 Prof. Hui Chen, Department of Conservative Dentistry and Periodontic, Affiliated Hospital of Stomatology, Medical College, Zhejiang University, 395# Yan An Road, Hangzhou, Zhejiang 310006, People's Republic of China. Tel: +86-57187217437; fax: +86-57187217433; e-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Summary Objective: This study was carried out to observe the enzymatic degradation of human dentin collagen fibrils exposed to exogenous collagenase in situ using atomic force microscopy, to understand the characteristics of the enzymatic degradation of collagen fibrils on dentin specimens. Methods: Polished dentin specimens from caries-free third molars were etched with citric acid, and then treated with an aqueous solution of 6.5% NaOCl for 120 s. The specimen was then put into a fluid cell and treated with a mixed solution of collagenase I (MMP-1) and collagenase II (MMP-8) for 9 h. AFM with contact mode was performed in situ to monitor the enzymatic degradation process of the dentin collagen fibrils. The distinctly topographic changes of the dentin surface were recorded continuously during different stages of the enzymatic degradation process. Results: The mixed solution of exogenous collagenase I and collagenase II could degrade dentin organic matrix (mainly collagen) efficiently, and the structures of dentin substrate were clearly exposed. Conclusion: It is possible to carry out real-time observations on the enzymatic biodegradation process of human dentin collagen fibrils on dentin specimens with atomic force microscopy in situ. 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Publication Year: 2011
Publication Date: 2011-01-11
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 19
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