Title: Transient rescue of cl virus from the persistently infected cell line Cl-E-8 by cocultivation
Abstract: The cell line Cl-E-8 showing expression of Sendai-694 viral antigens after the original isolation was reexamined after approximately 160 subcultures. In the virus fraction of cell supernatants 694 virus particles (termed 694 cl) could be demonstrated; no infectivity, however, was monitored. We were also unable to activate the viral infectivity of 694 cl virus by trypsin treatment. The analysis of viral RNA revealed that the virus contains a high-molecular-weight (50 S) single-stranded RNA. After cocultivation of Cl-E-8 cells with several standard cell lines the production of an infectious 6/94 virus, termed 694 co, was detected. The infectivity titer in the supernatants was very low, about three orders of magnitude lower than in cultures infected with the egg-grown 694 virus (694 ST). Surprisingly, the production of infectious 694 cl virus invariably ceased several subcultures after cocultivation even in the presence of foreign cells. However, the infectivity could be repeatedly reinduced by adding fresh foreign cells.
Publication Year: 1983
Publication Date: 1983-03-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 12
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