Title: Enzymic synthesis of RNA with reovirus RNA as template
Abstract: Reovirus RNA is a very effective template in RNA synthesis catalyzed by RNA polymerase from Escherichia coli B. With all four triphosphates present in the reaction mixture, there is synthesis not only of an RNA product with a base composition similar to that of the primer RNA, but also of polyadenylate and polyuridylate. Pancreatic ribonuclease rendered acid-soluble 50% of the [14C]CMP-labeled, reovirus RNA-primed product obtained after 4 minutes of synthesis. Reovirus RNA-primed incorporation of cytidine monophosphate into acid-insoluble product was inhibited 70% by 2 μg/ml. of actinomycin D. Reovirus RNA caused only a minimal depression in the absorption maximum of actinomycin D at 440 mμ, without a shift of the maximum to longer wavelengths.
Publication Year: 1964
Publication Date: 1964-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 55
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