Title: Studies on vaccinia virus plaque formation and its inhibition by interferon
Abstract: A plaque inhibition assay of interferon using vaccinia virus as challenge has been developed. No adsorption period of either interferon or virus was required; instead, both interferon and virus were added simultaneously to chick embryo cell monolayers in bottles without an agar overlay. After 44–48 hours of incubation at 37° the monolayers were stained with crystal violet and the plaques were counted. The dose-response relationship remained unaffected by variations in the amount of challenge virus between 50 and 19,000 PFU per monolayer. When the percentage plaque inhibition was plotted against the logarithm of the dose, different interferon preparations yielded parallel curves. The dose-response curves were sigmoidal and could be subjected to probit analysis. The method could be adapted to other tissues and other inhibitors.
Publication Year: 1963
Publication Date: 1963-03-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 95
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