Abstract: Fluorescent proteins (FPs) are a class of proteins that exhibit fluorescence as a consequence of fluorophore formation by an intramolecular reaction of the amino acid side chains contained within these proteins. The FPs can be modified to access specific cellular locations and specific physiological processes, and to act as specific ligands. The chapter discusses the evolutionary aspects and commercial availability of fluorescent proteins. A prerequisite to flow analysis is the ability to express sufficient quantities of FPs within the cell type of interest for the cells to be detectable using standard flow instrumentation. Flow analysis of (1) FPs–expressing cells, (2) fluorescence resonance energy transfer (FRET), and (3) FPs–accumulating organelles are the methods existing for transgenic expression in eukaryotic organisms. The emergence of novel flow-based assays for different cellular functions and components will continue, and improved sensitivity of end-point measurements permit lowering the total numbers of cells or subcellular organelles required to be purified by flow sorting. Further integration of these techniques is anticipated with established and emerging high-throughput methods, including genomics, proteomics, and metabolomics.
Publication Year: 2004
Publication Date: 2004-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 7
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