Title: [13] Translational regulation of chloroplast gene expression in chlamydomonas reinhardtii
Abstract: Translational regulation of chloroplast-encoded mRNAs has been studied extensively in the unicellular green alga Chlamydomonas reinhardtii. A number of nuclear-encoded proteins have been characterized within this organism that required for translation of specific chloroplast-encoded, photosynthesis-related mRNAs. These proteins have been found to act at different levels, including mRNA stability, processing, translation initiation, and elongation. This chapter describes methods used in laboratory to study translational regulation of chloroplast mRNAs that encode for photosystem II (PSII), proteins, with specific emphasis on the D1 protein, encoded by the psbA gene. The methods can be applied to studying translation of other chloroplast-encoded mRNAs within C. reinhardtii, and to some degree within higher plants. The chapter discusses the generation and selection of photosynthetic mutants of C. reinhardtii. One of the simpler methods utilized to generate nuclear mutants of C. reinhardtii is insertion of exogenous DNA into the nuclear genome. Use of a selectable marker gene allows for the growth of transformants on selectable media.
Publication Year: 1998
Publication Date: 1998-01-01
Language: en
Type: book-chapter
Indexed In: ['crossref']
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Cited By Count: 18
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