Title: Molecular Characterization of Microbial Communities in a JP-5 Fuel Contaminated Soil
Abstract: In this study, lipid biomarker characterization of the bacterial and eukaryotic communities was combined with PCR-DGGE analysis of the eubacterial community to evaluate correlation between JP-4 fuel concentration and community structure shifts. Vadose, capillary fringe and saturated-soils were taken from cores within, up- and down-gradient of the contaminant plume. Significant differences in biomass and proportion of Gram negative bacteria were found inside and outside the plume. Sequence analysis of DGGE bands from within the spill site suggested dominance by a limited number of phylogenetically diverse bacteria. Used in tandem with pollutant quantification, these molecular techniques should facilitate significant improvements over current assessment procedures for determination of remediation end points.
Publication Year: 1999
Publication Date: 1999-04-19
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot