Title: IN VITRO EVALUATION OF NEMATICIDAL ACTION OF NEEM AND BT FORMULATIONS ON MELOIDOGYNE INCOGNITA
Abstract: Background & Objectives: Meloidogyne incognita is a one of the major pathogens of tomato in India and cause severe
crop damage. M. incognita can be managed effectively by chemical treatments but many of the nematicides are expensive,
pose human and environmental risk. Management of M. incognita with biological control agents has been receiving
growing consideration. Therefore, in the present study neem and Bt were used to control M. incognita through egg hatching
experiment in laboratory conditions.
Methods: Nematicidal activities of neem and Bacillus thuringiensis have been investigated against root-knot nematode (M.
incognita) in laboratory conditions. For this purpose, the effects of aqueous extract of the neem leaf and seed alone, Bt alone
(whole cell suspension) and combination with each other on the hatch of eggs of Meloidogyne incognita were evaluation for
different time intervals (1 h to 72 h).
Results: The NSE inhibit the egg hatching at first hour was 50% which increased to 100% at 72 h. NLE was also effective
and egg hatch inhibition was 94.3% and 96.8% at 48h and 72h respectively. Egg hatch inhibition in standard concentrations
(100% w/v) of neem, increased with increase in incubation time from 1 h to 72 h. Effect of whole cell suspension (WCS)
(1.1X 10
9
CFU/ml) + neem seed extract on egg hatching after 12 h exposure was the most effective treatment and caused
significantly least number of egg hatching (0.2%) as compared to the water control. The present study indicated that WCS
formulation of Bacillus thuringiensis strain MTCC CODE 1953 was effective and caused significantly 100% egg hatch
inhibition after 72 h exposure as compared to the water control.
Publication Year: 2015
Publication Date: 2015-12-15
Language: en
Type: article
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Cited By Count: 1
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