Title: Determination of Protein Contacts by Chemical Cross‐Linking With EDC and Mass Spectrometry
Abstract:This unit describes a method for analyzing protein complexes by chemically cross-linking closely positioned amino acids. The cross-linked products are isolated by SDS-PAGE and the bands of interest ar...This unit describes a method for analyzing protein complexes by chemically cross-linking closely positioned amino acids. The cross-linked products are isolated by SDS-PAGE and the bands of interest are excised. Proteins in the excised gel piece are digested with trypsin and the resulting peptides recovered. Chemically bonded peptides are distinct from the multitude of unmodified single peptides by the presence of two carboxy-terminal ends. The protocol also describes incorporation of (18)O at the carboxy-terminal ends and purification and preparation of the peptide mixture for mass spectroscopy.Read More
Publication Year: 2007
Publication Date: 2007-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 6
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot