Title: CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF FLAVONOID-RICH EXTRACTS FROM LEAVES OF AMPELOPSIS GROSSEDENTATA
Abstract: Journal of Food BiochemistryVolume 33, Issue 6 p. 808-820 CHARACTERIZATION AND ANTIOXIDANT ACTIVITY OF FLAVONOID-RICH EXTRACTS FROM LEAVES OF AMPELOPSIS GROSSEDENTATA JIANHUA GAO, JIANHUA GAO College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorBENGUO LIU, Corresponding Author BENGUO LIU School of Food ScienceHenan Institute of Science and TechnologyHualan Street, Xinxiang 453003, China TEL: 86-373-3040674; FAX: 86-373-3040709; EMAIL: [email protected]Search for more papers by this authorZHENGXIANG NING, ZHENGXIANG NING College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorRUIXIANG ZHAO, RUIXIANG ZHAO School of Food ScienceHenan Institute of Science and TechnologyHualan Street, Xinxiang 453003, ChinaSearch for more papers by this authorAIYUAN ZHANG, AIYUAN ZHANG College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorQIONG WU, QIONG WU College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this author JIANHUA GAO, JIANHUA GAO College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorBENGUO LIU, Corresponding Author BENGUO LIU School of Food ScienceHenan Institute of Science and TechnologyHualan Street, Xinxiang 453003, China TEL: 86-373-3040674; FAX: 86-373-3040709; EMAIL: [email protected]Search for more papers by this authorZHENGXIANG NING, ZHENGXIANG NING College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorRUIXIANG ZHAO, RUIXIANG ZHAO School of Food ScienceHenan Institute of Science and TechnologyHualan Street, Xinxiang 453003, ChinaSearch for more papers by this authorAIYUAN ZHANG, AIYUAN ZHANG College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this authorQIONG WU, QIONG WU College of Light Industry and Food ScienceSouth China University of TechnologyGuangzhou, ChinaSearch for more papers by this author First published: 08 December 2009 https://doi.org/10.1111/j.1745-4514.2009.00253.xCitations: 58Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract ABSTRACT Leaves of Ampelopsis grossedentata have been consumed in southern China as health tea and herbal medicine. In this study, two kinds of flavonoid-rich extracts from leaves of A. grossedentata were prepared by traditional solvent extraction (product A) and recrystallization (product B). By analysis of ultraviolet-visible (UV) spectrometry, infrared (IR) spectrometry, electrospray ionization mass spectrometry (ESI-MS), nuclear magnetic resonance (NMR) spectrometry and high-performance liquid chromatography (HPLC)-ESI/MS, the major flavonoid in two products was identified as (+)-dihydromyricetin (DMY). Also, by HPLC, the contents of DMY in leaves, product A and product B were measured to be 32.96 ± 1.18%, 77.15 ± 1.98% and 95.12 ± 2.86%, respectively. Various antioxidant tests (1,1-diphenyl-2-picrylhydrazyl radical scavenging, reducing power, antioxidant activity in linoleic acid system) showed that the antioxidant activity of the flavonoid-rich extracts could be comparable with that of tertiary butylhydroquinone. The flavonoid-rich extracts from A. grossedentata leaves have a promise to be a new natural antioxidant with potential application in food industry. PRACTICAL APPLICATION Leaves of Ampelopsis grossedentata are consumed in southern China as health tea (Tengcha) and herbal medicine. In this study, the flavonoid-rich extracts from leaves of A. grossedentata, which were rich in (+)-dihydromyricetin, were prepared by traditional solvent extraction and recrystallization. Also, the antioxidant activity of the extracts can be comparable with that of tertiary butylhydroquinone. The results obtained in this study suggest that leaves of A. grossedentata may have potential as a new source of natural antioxidant and health food with great commercial interest in the food and phytopharmaceutical market. REFERENCES BURDA, S. and OLESZEK, W. 2001. Antioxidant and antiradical activities of flavonoids. J. Agric. Food Chem. 49, 2774– 2779. CANDAN, F., UNLU, M., TEPE, B., DAFERERA, D., POLISSIOU, M., SÖKMEN, A. and AKPULAR, H.A. 2003. Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). J. Ethnopharmacol. 87, 215– 220. DU, Q., CAI, W., XIA, M. and ITO, Y. 2002. Purification of (+)-dihydromyricetin from leaves extract of Ampelopsis grossedentata using high-speed countercurrent chromatograph with scale-up triple columns. J. Chromatogr. A 973, 217– 220. HOLLMAN, P.C.H., HERTOG, M.G.L. and KATAN, M.B. 1996. Analysis and health effects of flavonoids. Food Chem. 57, 43– 46. LI, Y., TAN, Z., LI, T., XIAO, B. and DAI, Q. 2006. Effects of Ampelopsis grossedentata on hyperlipidemia and myocardial enzymes in rats. Acta Nutrimenta Sinica 28, 506– 509. ŠKERGET, M., KOTNIK, P., HADOLIN, M., HRAŠ, A.R., SIMONIČ, M. and KNEZ, Ž. 2005. Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food Chem. 89, 191– 198. STRIVASTAVA, A., HARISH, S.R. and SHIVANANDAPPA, T. 2006. Antioxidant activity of the roots of Decalepis hamiltonii (Wight & Arn.). Lebensm.-Wiss. Technol.-Food Sci. Technol. 39, 1059– 1065. SUN, T. and HO, C. 2005. Antioxidant activities of buckwheat extracts. Food Chem. 90, 743– 749. VALENTÃO, P., FERNANDES, E., CARVALHO, F., ANDRADE, P.B., SEABRA, R.M. and BASTOS, M.L. 2003. Hydroxyl radical and hypochlorous acid scavenging activity of small centaury (Centaurium erythraea) infusion. A comparative study with green tea (Camellia sinensis). Phytomedicine 10, 517– 522. WATANABE, M., OHSHITA, Y. and TSUSHIDA, T. 1997. Antioxidant compounds from buckwheat (Fagopyrum esculentum Möench) hulls. J. Agric. Food Chem. 45, 1039– 1044. ZHANG, Y., NING, Z., YANG, S. and WU, H. 2003. Antioxidant properties and mechanism of action of dihydromyricetin from Ampelopsis grossedentata. Acta Pharmaceutica Sinica 38, 241– 244. ZOU, Y., LU, Y. and WEI, D. 2004. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. J. Agric. Food Chem. 52, 5032– 5039. Citing Literature Volume33, Issue6December 2009Pages 808-820 ReferencesRelatedInformation