Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1491122936', 'doi': 'https://doi.org/10.1002/0471739499.oth1', 'title': 'Methods and Protocols', 'display_name': 'Methods and Protocols', 'publication_year': 2005, 'publication_date': '2005-10-07', 'ids': {'openalex': 'https://openalex.org/W1491122936', 'doi': 'https://doi.org/10.1002/0471739499.oth1', 'mag': '1491122936', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/16335722'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1002/0471739499.oth1', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/0471739499.oth1', 'source': {'id': 'https://openalex.org/S2764845328', 'display_name': 'Methods of biochemical analysis', 'issn_l': '0076-6941', 'issn': ['0076-6941', '1934-4325'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'book series'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'review', 'type_crossref': 'other', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'bronze', 'oa_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/0471739499.oth1', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5108540856', 'display_name': 'Steven R. Kain', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I138285227', 'display_name': 'Agilent Technologies (United States)', 'ror': 'https://ror.org/02tryst02', 'country_code': 'US', 'type': 'company', 'lineage': ['https://openalex.org/I138285227']}], 'countries': ['US'], 'is_corresponding': True, 'raw_author_name': 'Steven R. Kain', 'raw_affiliation_strings': ['Agilent Technologies, Inc. 3500 Deer Creek Road Palo Alto, CA 94304, USA'], 'affiliations': [{'raw_affiliation_string': 'Agilent Technologies, Inc. 3500 Deer Creek Road Palo Alto, CA 94304, USA', 'institution_ids': ['https://openalex.org/I138285227']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': ['https://openalex.org/A5108540856'], 'corresponding_institution_ids': ['https://openalex.org/I138285227'], 'apc_list': None, 'apc_paid': None, 'fwci': 1.173, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 251, 'citation_normalized_percentile': {'value': 0.754569, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': None, 'issue': None, 'first_page': '407', 'last_page': '421'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T13127', 'display_name': 'Biotin and Related Studies', 'score': 0.8413, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T13127', 'display_name': 'Biotin and Related Studies', 'score': 0.8413, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10932', 'display_name': 'Microbial Metabolic Engineering and Bioproduction', 'score': 0.7813, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [], 'concepts': [{'id': 'https://openalex.org/C2778805511', 'wikidata': 'https://www.wikidata.org/wiki/Q1713', 'display_name': 'Citation', 'level': 2, 'score': 0.5121243}, {'id': 'https://openalex.org/C161191863', 'wikidata': 'https://www.wikidata.org/wiki/Q199655', 'display_name': 'Library science', 'level': 1, 'score': 0.5026784}, {'id': 'https://openalex.org/C166957645', 'wikidata': 'https://www.wikidata.org/wiki/Q23498', 'display_name': 'Archaeology', 'level': 1, 'score': 0.3472978}, {'id': 'https://openalex.org/C127413603', 'wikidata': 'https://www.wikidata.org/wiki/Q11023', 'display_name': 'Engineering', 'level': 0, 'score': 0.3425183}, {'id': 'https://openalex.org/C205649164', 'wikidata': 'https://www.wikidata.org/wiki/Q1071', 'display_name': 'Geography', 'level': 0, 'score': 0.32189554}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.19619271}], 'mesh': [{'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D010944', 'descriptor_name': 'Plants', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004331', 'descriptor_name': 'Drosophila melanogaster', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004331', 'descriptor_name': 'Drosophila melanogaster', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D049452', 'descriptor_name': 'Green Fluorescent Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006367', 'descriptor_name': 'HeLa Cells', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010944', 'descriptor_name': 'Plants', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D030821', 'descriptor_name': 'Plants, Genetically Modified', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D011994', 'descriptor_name': 'Recombinant Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013053', 'descriptor_name': 'Spectrophotometry', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': False}, {'descriptor_ui': 'D013053', 'descriptor_name': 'Spectrophotometry', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1002/0471739499.oth1', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/0471739499.oth1', 'source': {'id': 'https://openalex.org/S2764845328', 'display_name': 'Methods of biochemical analysis', 'issn_l': '0076-6941', 'issn': ['0076-6941', '1934-4325'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'book series'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/16335722', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1002/0471739499.oth1', 'pdf_url': 'https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/0471739499.oth1', 'source': {'id': 'https://openalex.org/S2764845328', 'display_name': 'Methods of biochemical analysis', 'issn_l': '0076-6941', 'issn': ['0076-6941', '1934-4325'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'book series'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 27, 'referenced_works': ['https://openalex.org/W1789456031', 'https://openalex.org/W1974288263', 'https://openalex.org/W1980073547', 'https://openalex.org/W1989760629', 'https://openalex.org/W1994021407', 'https://openalex.org/W2001496079', 'https://openalex.org/W2014293907', 'https://openalex.org/W2026896701', 'https://openalex.org/W2030927251', 'https://openalex.org/W2032579724', 'https://openalex.org/W2035843420', 'https://openalex.org/W2036742051', 'https://openalex.org/W2049793619', 'https://openalex.org/W2050848851', 'https://openalex.org/W2058254034', 'https://openalex.org/W2061104342', 'https://openalex.org/W2077835601', 'https://openalex.org/W2087823077', 'https://openalex.org/W2098998842', 'https://openalex.org/W2099152453', 'https://openalex.org/W2108407770', 'https://openalex.org/W2119612945', 'https://openalex.org/W2129494790', 'https://openalex.org/W2144259254', 'https://openalex.org/W2152442542', 'https://openalex.org/W2429768156', 'https://openalex.org/W2498454769'], 'related_works': ['https://openalex.org/W4317642157', 'https://openalex.org/W2899084033', 'https://openalex.org/W2748952813', 'https://openalex.org/W2363440148', 'https://openalex.org/W2352401039', 'https://openalex.org/W2317510431', 'https://openalex.org/W2181265275', 'https://openalex.org/W1990811139', 'https://openalex.org/W1974749013', 'https://openalex.org/W134931362'], 'abstract_inverted_index': {'Free': [0], 'Access': [1], 'Methods': [2], 'and': [3, 117], 'Protocols': [4], 'Steven': [5, 8, 29, 32, 66], 'R.': [6, 9, 30, 33, 64, 67], 'Kain,': [7, 31, 65], 'Kain': [10, 34, 68], 'Agilent': [11, 35, 69], 'Technologies,': [12, 36, 70], 'Inc.': [13, 37, 71], '3500': [14, 38, 72], 'Deer': [15, 39, 73], 'Creek': [16, 40, 74], 'Road': [17, 41, 75], 'Palo': [18, 42, 76], 'Alto,': [19, 43, 77], 'CA': [20, 44, 78], '94304,': [21, 45, 79], 'USASearch': [22, 46, 80], 'for': [23, 47, 58, 81], 'more': [24, 48, 59, 82], 'papers': [25, 49, 60, 83], 'by': [26, 50, 61, 84], 'this': [27, 51, 62, 85], 'author': [28, 52, 86], 'Book': [53], 'Editor(s):Martin': [54], 'Chalfie,': [55], 'Martin': [56], 'ChalfieSearch': [57], 'authorSteven': [63], 'First': [87], 'published:': [88], '07': [89], 'October': [90], '2005': [91], 'https://doi.org/10.1002/0471739499.oth1Citations:': [92], '6Book': [93], 'Series:Methods': [94], 'of': [95], 'Biochemical': [96], 'Analysis': [97], 'AboutPDFPDF': [98], 'ToolsRequest': [99], 'permissionExport': [100], 'citationAdd': [101], 'to': [102], 'favoritesTrack': [103], 'citation': [104], 'ShareShareShare': [105], 'a': [106], 'linkShare': [107], 'onFacebookTwitterLinked': [108], 'InRedditWechat': [109], 'Citing': [110], 'Literature': [111], 'Green': [112], 'Fluorescent': [113], 'Protein:': [114], 'Properties,': [115], 'Applications,': [116], 'Protocols,': [118], 'Volume': [119], '47,': [120], 'Second': [121], 'Edition': [122], 'RelatedInformation': [123]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1491122936', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 17}, {'year': 2020, 'cited_by_count': 18}, {'year': 2019, 'cited_by_count': 12}, {'year': 2018, 'cited_by_count': 7}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 7}, {'year': 2015, 'cited_by_count': 18}, {'year': 2014, 'cited_by_count': 13}, {'year': 2013, 'cited_by_count': 24}, {'year': 2012, 'cited_by_count': 25}], 'updated_date': '2024-12-09T03:03:24.373534', 'created_date': '2016-06-24'}